염증성 치은조직에서 Cell Adhesion Molecule의 발현에 관한 연구

박경근,김은철,유형근,신형식,Park, Kyung-Geun,Kim, Eun-Chul,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.3

The change in vascular adhesion molecule expression and number of infiltrating leukocytes were investigated irnmunohistochemically in clinically healthy and inflammed gingiva. Monoclonal antibodies to ICAM-1, VCAM-1 and E-cadherin were used to identify positive vessels and leukocyte within gingival biopsies. 10 healthy gingiva and 30 inflammed gingiva was resected by clinical crown lengthening and modified Widman flap operation, respectively. Leukocyte entry into tissues at sites of inflammation is controlled by the interaction between adhesion molecule and endothelium. Because of rapid and severe destructive periodontal disease that is remarkable leukocyte adhesion deficiency, it is very important to unerdstand the mechanism of host defence against periodontal disease. The purpose of this investigation was the characterization of the presence and distribution of the adhesion molecule(ICAM-1, VCAM-1 and Evcadherin) in inflammatory gingival tissues compared to clinically healthy gingiva. The results were as followed; 1. ICAM-1 was distributed on basal layer, endothelium and mononuclear cells 10 healthy gingiva but inflammed gingiva was observed stronger stain than healthy gingiva. 2. Rare expression was observed in both group but few positive VCAM-1 cells were investigated in inflammatory gingival tissues 3. E-cadherin was expressed in only epithelium and reduced expression was observed in inflammatory gingival tissues. ICAM-1, VCAM-1 showed more expression in inflammatory tissues compared to healthy gingiva. Conversely, E-cadherin revealed a opposite result. These finding demonstrate a characteristic distribution and degree of adhesion molecule in healthy and inflammatory gingival tissues. But it is suggested that more detail study be progressive associated with leukocyte adhesion molecule to determine characterization of periodontal disease.

노인장기요양보험제도 재가급여 이용자의 서비스 만족도 연구

박경근(Park Kyung Keun) 한국복지실천학회 2016 한국복지실천학회지 Vol.7 No.1

This thesis made a study on the satisfaction of Home Visiting Care and Visiting Bath Service users in Namyangju area by studying the 123 of people(male 28 person, female 95 person) using Long-term Care Insurance for the elderly who pay home care service fee. Also, satisfaction with the level of satisfaction by analyzing Long-Term Care Insurance for pensioners receiving services with the development of long-term care insurance for the elderly look at the common characteristics imply and is aimed at. Service satisfaction and welfare benefits, and visit care services and users in home elderly respondents demographic and economic characteristics, and type of service, in order to achieve the purpose of the present study, SPSS statistical characteristics of subjects Satisfaction regression analysis was performed using descriptive statistics, T-test, ANOVA, correlation program. The results of the study are as follows. For the first, the Long-Term Care Insurance user's satisfaction of service contract and all of thing about home visiting care service is divided into 3 subordinate. And suggest each of below factor, question average and standard deviation. The satisfaction of service is average 4.68. As above factor, Satisfaction of home care service is question of 17 of physical activity service(to wash her/him hair, to feed meal, to help her/him bath and so on). Satisfaction of the physical activity service is the most of high percent 4.86. Mentality service(to talk and listen, to counsel and communicate) is 4.69. And last thing that worker keeps user's secret to be exposed during home care service is 4.67. As below factor, Satisfaction of dementia management service is 4.04%. And user's satisfaction of worker's effort. Physical activity service is most of top average as 4.05 and mentality service is average 4.40, personal life service is average 4.46, daily life service is 4.48 on question about home care service. The result shows that home care service is important. Because The result focused on that home care service is more important than out care service like to help go shopping, to help eat out that the demand is small. It is long-term investment to be able to prevent from going out home care service. Second, the difference in home care service user's satisfaction level depending on characteristic of sociology of population shows that the difference between subordinate concepts of each variable is statistically meaningless. But subordinate concepts of each group's difference is meaning. Third, the difference in the satisfaction of home care service users depending on service using pattern did survey 4 question of service user, period, using number, payer. The user's satisfaction is higher when worker services home care service, the using period 12~18months and number of service of 5 days a week. The service fee pay user's son or daughter-in-law. Lastly, analysis of the effect of service user's characteristics on the satisfaction shows that user's characteristic of sociology of population and characteristic of service using pattern affect on the satisfaction of users. In other words, religion, gender, user, period of service use, frequency of service use per week, time of service use per day and total amount of actual costs have an effect on the satisfaction level. As the results, There are some suggestion about policy, practical proposals, and follow-up study. Also, long-term insurance for elderly contribute to not welfare policy for senior but the elderly life. 본 연구는 노인장기요양보험제도가 시행된 2008년 7월 1일부터 경기도 남양주시에서 재가복지 장기요양서비스를 받은 급여 대상자 남자 28명, 여자 95명 전체 123명을 대상으로 직접 면접을 통한 설문을 통해 자료 수집하였으며 제공자가 방문요양과 방문목욕 장기요양서비스를 제공할 때 서비스의 만족도에 대하여 연구 하였다. 또한 장기요양보험제도 이용자가 서비스를 받으면서 만족도의 수준과 만족도를 분석함으로 노인장기요양보험제도의 발전과 일반적인 특성을 살펴보고 함의를 제시하는데 목적이 있다. 본 연구의 목적 달성을 위해 서비스 이용 만족도와 재가복지급여 이용자 및 방문요양서비스를 이용하고 있는 노인을 대상으로 조사대상자의 인구사회학적특성과 서비스 이용형태 특성 대상자의 특성이 만족도에 미치는 영향을 SPSS 통계 프로그램 이용하여 기술 통계 T-test, ANOVA, 상관관계 회귀분석을 진행하였다. 첫째, 장기요양보험제도 대상자의 이용자만족도를 서비스 계약 만족도 및 재가 급여 서비스 만족도, 그리고 전반적 만족도등 3개 3의 하위요인으로 나누고 각 하위 요인별 및 문항별 평균과 표준 편차를 제시 하였고 서비스계약만족도 평균은 4,68으로 나타났다. 하위요인으로서 재가 급여 서비스 만족도는 하위요인을 구성하고 있는 17개문항 중 신체활동지원 서비스가 만족한다. (머리감기기, 식사, 화장실, 목욕도움등)가 4,86로서 가장 높았으며 정서지원 서비스가 만족한다. (말벗, 격려, 위로, 생활상담, 의사소통)가 4,69 높게 나타났으며 제공자는 서비스를 제공 하면서 알게 된 비밀을 잘 지켜서 만족 한다가 4,67 비교적 높게 나타났다. 낮은 점수를 나타낸 문항으로는 치매관리 지원서비스가 만족한다.(행동변화 대처)가 4,04로 나타났고 다음으로 제공자가 이용자가 원하는 서비스를 잘 해주어서 만족 한다가 4,05로 낮게 나타났다. 전반적인 만족도 조사에서는 기관과 계속적으로 관계를 맺으며 서비스를 이용하고자 한다가 4,40로 비교적 보편적으로 나타났다. 또 장기요양 서비스를 받기 전 보다 서비스 받은 후 이용자의 건강과 삶의 질이 향상되어 만족한다가 4,46로 높게 나타났으며 재가급여 서비스에 대해서 전반적으로 만족한다가 4,48로 비교적 높은 편이다. 둘째, 방문요양 서비스이용자의 인구사회학적특성에 따른 만족도 차이를 분석한 결과 각 변인들의 하위집단간의 만족도 차이는 통계적으로 유의미하지 않는 것으로 나타났으나 하위요인별 집단별 차이는 있는 것으로 나타났다. 셋째, 장기요양급여 대상자의 특성이 만족도에 미치는 영향을 분석한 결과 서비스 이용자의 인구 사회학적 특성과 서비스 이용형태 특성이 만족도에 미치는 것으로 나타났다. 즉 종교, 성별 이용자 유형이 주 횟수에 대한 비용부담과 만족도에 대한 영향을 주는 것으로 나타났다. 특히 서비스 이용기간과 횟수는 만족도에 상당한 영향을 주는 것으로 나타났다. 이와 같은 결과를 토대로 정책적 실천적 그리고 후속 연구에 대한 제언이 제시 되었다. 또한 노인장기요양보험제도가 노인들의 삶 뿐 아니라 우리나라 노인복지정책의 효과성을 창출하는데 궁극적으로 크게 기여 할 수 있을 것이다.

평행 광축에서의 위상측정 형상측정법에 관한 연구

정경민(Kyung-min Jeong),박윤창(Yoon-chang Park),박경근(Kyung-keun Park) Korean Society for Precision Engineering 2000 한국정밀공학회지 Vol.17 No.6

회전다면경을 이용한 줄무늬 격자 영사방법에 관한 연구

박윤창(Yoon-chang Park),정경민(Kyung-min Jeong),장석준(Seok-jun Jang),박경근(Kyung-keun Park) Korean Society for Precision Engineering 2001 한국정밀공학회지 Vol.18 No.6

영사식 3차원 형상 측정을 위한 격자무늬 생성장치에 관한 연구

박윤창(Yoon-chang Park),박경근(Kyung-keun Park) 한국생산제조학회 1999 한국공작기계학회 추계학술대회논문집 Vol.1999 No.-

그림자식 모아레를 이용한 형상측정법의 정확도 개선에 관한 연구

박경근,박윤창,정경민 한국공작기계학회 1999 한국공작기계학회 춘계학술대회논문집 Vol.1999 No.-

Generally, When we measure of object 3D surfaces with phase shifting shadow moire method. It is use of optical system consist of light source, grating, and ccd camera. At this time, It is important parameter that vertical distance of grating and camera, grating and light source, and horizontal distance of camera and light source. When use camera consist of complex lens vertical distance of grating and camera is unknown parameter. From this cause equivalent wave length of moire fringe is uncertain. In this study, We exactly obtain a vertical distance of grating and camera so improve on measurement accuracy.

염증성 치은조직에서 부착단백의 발현에 관한 연구

박경근,유형근,신형식 원광대학교 치의학연구소 1996 圓光齒醫學 Vol.6 No.3

The change in vascular adhesion molecule expression and number of infiltrating leukocytes were investigated immunohistochemically in clinically healthy and inflammed gingiva. Monoclonal antibodies to ICAM-1, VCAM-1 and E-cadherin were used to identify positive vessels and leukocyte within gingival biopsies. 10 healthy gingiva and 30 inflammed gingiva was resected by clinical crown lengthening and modified Widman flap operation, respectively. Leukocyte entry into tissues at sites of inflammation is controlled by the interaction between adhesion molecule and endothelium. Because of rapid and severe destructive periodontal disease that is remarkable leukocyte adhesion deficiency, it is very important to understand the mechanism of host defence against periodontal disease. The purpose of this investigation was the characterization of the presence and distri bution of the adhesion molecule( ICAM-1, VCAM-1 and E-cadherin ) in inflammatory gingival tissues compared to clinically healthy gingiva. The results were as followed; 1. ICAM-1 was distributed on basal layer, endothelium and mononuclear cells in healthy gingiva but inflammed gingiva was observed stronger stain than healthy gingiva. 2. Rare expression was observed in both group but few positive VCAM-l cells were investigated in inflammatory gingival tissues 3. E-cadherin was expressed in only epithelium and reduced expression was obse rved in inflammatory gingival tissues. ICAM-1, VCAM-l showed more expression in inflammatory tissues compared to healthy gingiva. Conversely, E-cadherin revealed a opposite result. These finding demonstrate a characteristic distribution and degree of adhesion molecule in healthy and inflammatory gingival tissues. But it is suggested that more detail study be progressive associated with leukocyte adhesion molecule to determine characterization of periodontal disease.

이황화탄소 중독자들의 노출중단 이후의 심박동수 변이

이상윤,조성일,백도명,변창범,김미정,박경근,임상혁,양길승,황창국,전형준 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 2000년도에 이황화탄소 중독증 환자들과 대조군의 심박동수 변이를 비교하여 이황화탄소 중독증 환자들의 심박동수 변이가 대조군에 비해 유의하게 낮다는 연구보고가 있었다. 당시의 연구가 이황화탄소 노출이 중단된 상태에서 이황화탄소 중독자들의 건강 문제를 연구했다는 점에 의의가 있으나 연구 결과를 해석하는데 있어 제한점들이 있었다. 이 연구는 2000년도에 수행하였던 연구의 제한점을 극복하고 이황화탄소 노출이 중단된 이후의 이황화탄소 중독자들의 심혈관계 상태를 심박동수 변이를 이용하여 평가해보고자 하였다. 방법: 이황화탄소 중독증으로 진단받은 환자 122명과 환자군의 연령대와 성별 빈도에 따라 무작위 추출한 112명을 대조군으로 선정하였다. 자기 기입식 설문지와 건강검진을 통해 연령, 성별, 신장, 체중, 흡연, 음주, 규칙적 운동, 질병력, 직업력, 흉부 방사선 촬영, 심전도 측정을 실시하였다. 연구 대상자들은 의자에 앉힌 상태에서 5분 동안 심박동수 변이를 측정하였다. 이 연구에서 측정한 심박동수 변이 지표들은 정상 RR간격의 표준편차(standard deviation of all norma-to-normal intervals, SDNN), 인접한 RR간격들의 차이를 제곱의 합의 평균의 제곱근(square root of the mean of the sum of squares of differences between adjacent normal-to-normal intervals, RMSSD). 저주파영역(low frequency power, LF: 0.04~0.15Hz), 고주파 영역 (high frequency power, HF:0.15~0.4Hz), 전체 주파수 강도(total power, TP), 저주파/고주파 비 (LF/HF ratio)이다. 결과: 단변량 분석에서 이황화탄소 중독자들의 모든 심박동수 변이 지표들이 대조군에 비하여 낮았다. 다중선형회귀분석에서 이황화탄소 중독은 RMSSD를 유의하게 감소시키는 것으로 나타났다.(P<0.05). 결론: 이 연구는 이황화탄소 노출이 중단된 상태에서도 이황화탄소 중독자들에게 심혈관계와 관련된 건강문제가 있을 수 있음을 시사한다. Objectives: A previous study conducted in 2000 measuring the heart rate variability (HRV) of carbon disulfide (CS_(2))-poisoned subjected suggested that their HRV was reduced after exposure cessation. However, the study was limited by the following procedural limitations: (1) only 71 CS_(2)-poisoned subjects participated, (2) no females participated, and (3) the CS_(2)-poisoned subjects were older than the controls. This study was therefore conducted to overcome these limitations of the earlier study. Methods: The study subjects comprised 122 retired workers with CS_(2) poisoning and the same number of age- and sex-matched controls. Information on individual age, sex, height, wight, smoking history, alcohol drinking, regular exercise, medical and occupational history, chest x-ray, and ECG recording of the two groups was collected though a self-administered questionnaire and a medical examination. Standard Deviation of NN intervals (SDNN), Root-Mean-Square of Successive Differences (RMSSD), Total Power (TP), Low Frequency (LF), High Frequency (HF), and LF/HF ratio were measured as HRV indices for 5 minutes in the sitting position. Results: Univariate analysis revealed that all HRV indices of CS_(2)-poisoned subjected were lower than those of the controls. Multiple linear regression analysis showed that CS_(2) poisoning had negative association with all HRV indices and that its association with RMSSD was statistically significant(P<0.05). Conclusions: This study suggests that CS_(2)-poisoned subjects continue to have reduced HRV, even though the exposure has ceased.

수 종의 생약이 치은 섬유모세포에 미치는 영향

유형근,박경근 원광대학교 치의학연구소 1996 圓光齒醫學 Vol.6 No.1

Periodontal regeneration reqiures the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the polypeptide growth factors PDGF, TGF, FGF, and IGF are mediators of these cellular events in wound healing. The purpose of this study is to examine the effects of 6 herbal drugs, radix ginseng, radix glycyrrhizae, radix scutellariae, rhizoma coptidis, flos sophorae, and tuber bletillae, on human gingival fibroblast. Human gingival fibroblasts were primarily cultured from extracted third molar with non-periodontal disease. The powder from extracted herbal drugs were prepared with distilled water. Cells were inoculated into the 24 well plate with 1×10^4ells/well of medium at 37℃, 5% of CO_2, 100% of humidity incubator for 24 hours. After change the medium, cells were treated with each herbal drugs concentrated with 10^-6g/㎖, l0^-6g/㎖ , and 10^-8/㎖ for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MIT assay solution. There was the most significant elevation in rhizoma coptidis and radix glycyrrhizae of 10^-10g/㎖ at 2, 3days(P<0.05). The result of this study demonstrated that rhizoma coptidis and radix glycyrrhizae appears to have beneficial effect on gingival fibroblast than any others.

평판배지법에 의한 4-chlorobenzoate 탈염소화 세균의 검색

채종찬,김치경,민경희,박경근 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1

Pseudomonas sp. DJ-12와 genomic DNA로부터 pBluescript SK(+)를 vector로 사용하여 4CBA의 탈염소화 유전자를 E. coli XL1-Blue에 클로닝하여 E. coli CJ1을 얻었다. 재조합 플라스미드인 pCJ1에는 4CBA의 탈염소화 유전자를 포함하는 약 9.5 kb의 insert DNA가 삽입되어 있었으며, 1개씩의 ApaI, HindIII, KpnI 인식부위와 2개의 AccI 인식부위가 존재하였다. pCJ1으로부터 4CBA의 탈염소화 유전자를 포함하는 약 3.4 kb의 EcoRI-HindIII 절편을 다시 subcloning 하여 E. coli CJ101을 얻었다. 이 4CBA의 탈염소화 기능을 갖는 재조합 균주들은 4CBA가 첨가된 Cl^--free 평판배지에서 균을 배양한 후, 0.1 M AgNO_3 용액을 첨가할 때 균체 집락의 주위에 생성되는 AgCl precipitate의 양으로 간편하게 검색할 수 있었다. 이와 같은 검정방법은 1 mM의 4CBA가 포함된 Cl^--free 평판배지에서 균체를 2일간 배양한 후 0.1 M AgNO_3를 첨가하는 조건으로 표준화하였다. The gene responsible for dechlorination of 4-chlorobenzoate (4CBA) was cloned in E. coli XL1-Blue from Pseudomonas sp. DJ-12. The cloned cell of E. coli CJ1 had the hybrid pBluescript SK(+) plasmid, into which about 9.5 kb genomic DNA fragment of Pseudomonas sp. DJ-12 was inserted. The subclone of pCJ101 was constructed by inserting the 3.4 kb EcoRI-HindIII fragment of pCJ1 into the vector. Those cloned cells could be simply selected by halo formation around the colonies which was the precipitate of AgCl produced by reaction of AgNO_3 and chloride ion liberated by bacterial dechlorination of 4CBA. Such a plate assay method was standardized by the procedure that colonies grown for 2 days on the Cl^--free plate medium containing 1 mM 4CBA were flooded with 0.1 M AgNO_3 solution.