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김홍선,최세영 한국고무학회 1994 엘라스토머 및 콤포지트 Vol.29 No.3
The Purpose of this dissertation is to study the preparation technique and physical properties of expanded rubber. The respective samples of NR, NBR and the standard compounds of EPDM rubber were prepared by mixing plasticizer DOP and LCR in various ratio. And their vulcanization characteristics, physical properties and foaming states were studied. Vulcanization characteristics were investigated using cure curve that had been obtained from Oscillating Disk Rheometer study, and the microscopic photographs of foaming states were obtained using electron microscope. The vulcanization characteristics of the samples, DOP 30phr and LCR 30phr, were found to be suitable. The tests, like hardness test and resilience, tensile test on the physical properties of each sample showed that plasticizing efficiencies of DOP and LCR were almost the same. Oil resistance test exhibited DOP was better than LCR and compression set test showed LCR was excellent plasticizes. The foaming states of NR, EPDM compounds were uniform, but those of NBR compounds were not uniform. Consequently vulcanization characteristics, physical properties and foaming states of all the samples satisfied the SAE requirements.
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감귤해충의 종합적방제에 관한 연구(2) 루비붉은좀벌(Anicetus beneficus)도입에 의한 루비깍지벌레 (Ceroplastes rubens)의 방제효과
김홍선,문덕영,박중수,이승찬,장영덕,Kim H.S.,Moon D.Y.,Lee S.C.,Kim H.S.,Lippold P. C.,Kim H.S. 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.2
기생봉인 루비붉은좀벌(Anicetus beneficus)을 1975년 일본에서 도입하여 제주도에 방사시키고 루비깍지 벌레 방제를 위한 생물학적 방법으로서의 가치를 평가한후 다음과 같은 결과를 얻었다. 1. 감귤의 주요해충인 루비깍지벌레는 년 1회 발생하였고 부화시류는 6월하순부터 8월초순이었고 부화최성기는 7월 중순이었다. 2. 루비붉은좀벌은 1975년 방사이래 16개 지역에 분포되었다. 3. 루비붉은좀벌의 기생율증가로 루비깍지벌레의 밀도가 현저하게 감소되었고 평균기생율은 1975년 $1.7\%$ 1976년 $14.\%$ 1977년 $31\%$ 1978연에는 $37\%$였다. An parasite, Anicetus beneficus was introduced from Japan to Jeju-do, Korea in 1975, and evaluated as a biological agent for control of the ruby scale, Ceroplastes rubens MASKELL. The results were as follows: 1. A major pest of citrus, ruby scale had one generation a year and hatched from late June to early August with the peak hatching in the middle of July. 2. A. beneficus was widely distributed in 16 citrus growing areas since released in 1975. 3. The pnupulation of ruby scales was considerably decreased by increasing of the parasites. Mean percent parasitism of A. beneficus was 1.7 in 1975, 14.1 in 1976, 31.0 in 1977 and 37.0 in 1978.
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김홍선,문덕영,장영덕,박중수,Kim H. S.,Moon D. Y.,Llippold P. C.,Chang Y. D.,Park J. S. Korean Society of Applied Entomology 1978 한국식물보호학회지 Vol.17 No.1
감귤해충 종합방제 확립을 위한 기초자료를 얻고저 제주도 서귀포에서 1973-1976년까지 4년간 실시한 결과 다음과 같은 결과를 얻었다. 1. 귤응애는 7철 중순에서 8철 중순 사이에 11회의 큰 peak를 나타냈다. 2. 한편 관행살포구에서는 7,8월뿐 아니라 9-11월에도 높은 밀도를 나타냈다. 3. 귤응애의 천적으로 Oligota yasumatsui K., Anysits baccarum L., Hemerobiid sp. Semidalis albate E., Orius sp., Agistemus terminalis Q., 무당벌레류 3종 포식응애 1종등 10종이 발견되었다. 4. 귤응애의 년도별 발생경향을 보며 무살포구에서는 매년 감소하는 경향이고 관행살포구에서는 계속 증가하는 경향이었다. 5. 살균제로서 석회볼드액과 동수화제 처리구는 귤응애밀도가 높았으나 항생제인 Streptomycin은 낮은밀도였다. Experiments were conducted to study the integrated control of one of the major pests of citrus, the citrus red mite, Panonychus citri (McGregor). Studies were conducted in Seogwipo, Cheju Island, 1973-1976. Results obtained were: 1. The major peak of citrus red mite occurrence was mid-July to mid-August. 2. Standard field populations of citrus red mite were also high in September, October, and November. 3. A total of 10 species of natural enemies of citrus red mite were found. These included; Oligota yasumatusi Kistner, Anystis baccarum L., Hemerobiid sp., Semidalis albate E., Orius sp., Agistemus terminalis Q., 3 species of lady beetles (Coccinellidae), and one unidentified species of predacious mite. 4. Annual occurrence of citrus med mite tended to decrease in unsprayed fields, but increased in fields receiving standard treatments. 5. With fungicides, there was an increase in mite populations associated with use of Bordeaux mixture and copperpowder sprays. Streptomycin, however, did not effect on increase in mite numbers.
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Kinetic Mechanism of Glycolate Oxidase from Spinach
김홍선,최정도,Kim, Hong-Seon,Choi, Jung-Do 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4
시금치 잎으로 분리한 glycolate oxidase의 속도론적 성질을 정류상태 (steady-state condition)에서 연구하였다. 고등식물의 glycolate oxidase는 glycolate 산화반응 이외에도 L-lactate와 glyoxylate 산화반응에도 촉매역활을 수행한다. 본 연구에서는 제 2의 기질로 2,6-dichlorophenol-indophenol (DCPI)와 $O_2$를 이용하여, steady-state 조건에서 glycolate, L-lactate 산화반응에 대한 반응 속도흔적 연구를 수행하였다. 기질의 여러 농도에서 측정한 반응속도의 이중-역수 작도는 모든 조건에서 교차형(intercepting pattern)을 보여 주므로 glycolate oxidase 반응은 ternary complex를 거치는 sequential 메카니즘으로 판단된다. Steady-state에서의 반응 속도론적 상수, $K_s$, $Ko_2$, $K_{DCPI}$, and $K_d$ 등은 이중-역수 작도의 절편과 기울기의 2차 작도로 부터 구하였다. [2-$^2H$]-lactate을 이용하여 중수소 동위원소의 효과를 조사한 결과, $V_H/V_D$는 pH에 따라 변하며, pH 6-9 범위에서 $V_{H}/V_{D}$가 5-3이 얻어졌다. 그러므로 반응속도 결정단계는 pH에 따라 변하며, 산성조건에서 C-H 결합의 분열단계가 주된 반응속도 결정단계로 여겨진다. The kinetic properties of glycolate oxidase from spinach have been studied under steady-state conditions. Glycolate oxidase from higher plants catalyzes the oxidation of glyoxylate and L-lactate in addition to glycolate oxidation. In this work we have carried out the kinetic studies of the oxidation of glycolate and L-lactate by glycolate oxidase with sodium 2,6-dichlorophenol-indophenol (DCPI) and oxygen as an electron-acceptor. Under steady-state conditions with different concentrations of substrate and electron-acceptor, intercepting patterns are observed in double-reciprocal plots. This is suggestive of a ternary complex (sequential) mechanism. Kinetic parameters, $K_s$, $Ko_2$, $K_{DCPI}$, and $K_d$ are obtained from secondary plots of intercepts and slopes of double-reciprocal plots. Deuterium isotope effect ($V_H/V_D$) with [2-$^2H$]-lactate were observed to be pH dependent, and the value of $V_H/V_D$ were varied from 5 to 3 in the pH range between 6.0 and 9.0. This is indicative that rate-limiting step is pH dependent and C-H bond-breaking is major rate-limiting step in acidic condition.
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Right Lower Sleeve Bilobectomy for Lung Cancer with Posteparterial Tracheal Bronchus
김홍선,김진식,조종호,신수민,김홍관,김진국 대한흉부외과학회 2017 Journal of Chest Surgery (J Chest Surg) Vol.50 No.4
A 42-year-old man was diagnosed with cancer of the right lower lung lobe with a posteparterial type of tracheal bronchus, in which the posterior segmental bronchus of the right upper lobe arose from the distal bronchus intermedius. A mass involved the distal bronchus intermedius, requiring a right lower bilobectomy with an additional posterior segmental resection of the right upper lung lobe. Thus, we performed a right lower bilobectomy and sleeve anastomosis of the posterior segmental bronchus of the right upper lobe to the proximal bronchus intermedius, sparing the pulmonary parenchyma of the same lobe.
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