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적응 요인에 따른 보조부화술 (Assited Hatching, AH)의 효과
김지수,강승호,권윤정,손인표,최규완,김수경,전한식,이제규,이승재,박종민,Kim, J.S.,Kang, S.H.,Kwon, Y.J.,Son, I.P.,Choi, K.W.,Kim, S.K.,Chun, H.S.,Lee, J.G.,Lee, S.J.,Park, J.M. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2
Implantation rates remain low following human in vitro fertilization (IVF). Suboptimal culture conditions may limit the ability of embryos to hatch as blastocysts, and artificial opening of the zona pellucida has been proposed as a means to promote subsequent hatching (assisted hatching). In this study, assisted hatching (AH) by zona drilling using acidic Tyrode's solution was performed in 320 patients, due to their age of more than 38 years (group A), the thick zona pellucida (group Z; $ZP\geq0.18{\mu}m$), and failures in implantation more than 3 times in previous IVF-ET trial (group P). This study was designed firstly, to study the effects of AH on the outcomes of IVF-ET according to the indications and secondly, to verify the appropriate application of AH. The results were as follows; 1. There was no difference in pregnancy rate between AH group (26.6%) and non-AH group (26.5%). 2. Assisted hatching (AH) showed significantly higher pregnancy rate of the patients with thick zona pellucid a than those of the patients with age factor and with the history of repeated implantation failure. But in the patients with age factor only, AH resulted in higher pregnancy rate. 3. Interestingly, the patients with complex factors including zona factor (Z: 33.9%; ZA: 30.4%; ZP: 31.6%; ZAP: 21.4%) showed higher pregnancy rates than other complex factors excluding zona factor (A: 24.4%, P: 0%; AP: 10.8%). From these results, AH is more helpful to the patients with thick zona pellucida rather than patients with older age and/or previous repeated implantation failure.
김지수,김영철,김용환,Anne J. Anderson 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.2
The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.
J. Kim(김지수),J. Jang(장진아),J. Kim(김종민),G. Ge(그가오),J. Won(원재연),D.-W. Cho(조동우) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
The clinical therapy for retinal vascular diseases requires repeated intravitreal injections of anti-vascular endothelial growth factors due to their short half-life, which imposes burdens such as endophthalmitis and hemorrhage for patients. Therefore, the improvement of drug delivery system is necessary to prolong the drug activity and minimize secondary complications. In this study, we used co-axial printing technique to develop a core/shell drug-loaded rod (Drug Rod) which could deliver dual types of drugs (Bevacizumab (BEV) and Dexamethasone (DEX)) from the same implant. The drug rod can achieve sustained and burst release kinetics of BEV and DEX, respectively, under both the in vitro and in vivo conditions. The versatility of coaxial printing technique facilitates to produce the drug rod with various dosages and dimensions that can be implanted in the vitreous using small gauge of the needle less-invasively. We validated the superiority of the implanted drug rod by using rat laser-induced choroidal neovascularization models for inhibiting inflammatory responses and long-term suppressing neovascularization compared to the conventional intravitreal injection of BEV. These results suggested that the drug rod could be an alternative therapeutic approach for the treatment of various types of retinal vascular diseases including AMD.