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이산화탄소로부터 생물전기화학적 아세트산 생산을 위한 미생물 농화배양 및 군집 분석
김준형(Junhyung Kim),김영은(Young-Eun Kim),박명화(Myeonghwa Park),송영은(Young Eun Song),설은희(Eunhee Seol),김중래(Jung Rae Kim),오유관(You-Kwan Oh) 한국신재생에너지학회 2020 신재생에너지 Vol.16 No.1
Microbial electrosynthesis has recently been considered a potentially sustainable biotechnology for converting carbon dioxide (CO₂) into valuable biochemicals. In this study, bioelectrochemical acetate production from CO₂ was studied in an H-type two-chambered reactor system with an anaerobic microbial consortium. Metal-rich mud flat was used as the inoculum and incubated electrochemically for 90 days under a cathode potential of -1.1 V (vs. Ag/AgCl). Four consecutive batch cultivations resulted in a high acetate concentration and productivity of 93 m㏖/L and 7.35 m㏖/L/day, respectively. The maximal coulombic efficiency (rate of recovered acetate from supplied electrons) was estimated to be 64%. Cyclic voltammetry showed a characteristic reduction peak at -0.2~-0.4 V, implying reductive acetate generation on the cathode electrode. Furthermore, several electroactive acetate-producing microorganisms were identified based on denaturing- gradient-gel-electrophoresis (DGGE) and 16S rRNA sequence analyses. These results suggest that the mud flat can be used effectively as a microbial source for bioelectrochemical CO₂ conversion.