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김일섭,넝탕부,후앙텅부,최기영,김영식 (사) 한국생물환경조절학회 2015 시설원예‧식물공장 Vol.24 No.1
This study was conducted to evaluate influence of short-term application of abscisic acid (ABA) in nutrientsolution on growth and drought tolerance of tomato seedlings. The treatments included four ABA concentrations(0.5, 1, 2, 3mg·L-1) and control (non-treatment) were applied to the nutrient solution in a hydroponic system. On the5th and 10th day after growing in the nutrient solution containing ABA, seedlings were transferred to -5 bars of PEG-8000 in a growth chamber to induce water stress. Except for stem diameter and fresh and dry weight of root, therewere no statistical differences in other growth parameters among control, 0.5 and 1mg·L-1 of ABA treatments. Seedlingsgrowths were strongly inhibited in nutrient solution containing 2 and 3mg·L−1 of ABA. The root growth such asfresh and dry weigh of root, total root surface area, and average root diameter was slightly enhanced in 1mg·L−1 ofABA treatment. The elevation of ABA concentrations in nutrient solution resulted in the decrease in transpirationrate and increase in stomatal diffusive resistance and leaf temperature of tomato seedlings. The initiations of seedlingwilting after treating in -5 bars of PEG were delayed from 10 hrs in control to 30 hrs in ABA applied treatments. Additionally, the high percentages of recovered seedlings were observed in 0.5 and 1mg·L−1 of ABAtreatments after re-irrigation. Therefore, short-term application of 1mg·L−1 of ABA in the nutrient solution stimulatedthe root growth and drought tolerance of tomato seedlings by delaying the start time of wilting point andenhancing the recovery after re-irrigation.
김일섭,신선영,김선형,윤호성 한국미생물학회 2012 The journal of microbiology Vol.50 No.3
The MuS1 gene is highly homologous to many stress-related proteins in plants. Here, we characterized whether a new candidate gene, MuS1, is related to multiple stress tolerance in yeast as it is in plants. Transgenic yeast strain expressing MuS1 were more resistant to hydrogen peroxide, menadione, high salinity, metals (i.e., cadmium, copper, iron, and zinc), ethanol, and lactic acid than wild-type strain transformed with a vector alone. In addition, the alcohol yield of the transgenic yeast strain was higher than that of the wild-type strain during the batch fermentation process. These results show that MuS1-expressing transgenic yeast strain exhibits enhanced alcohol yield as well as tolerance to abiotic stresses, especially metal stress.
김일섭,Hye-Youn Moon,Hae-Sun Yun,진익렬 한국미생물학회 2006 The journal of microbiology Vol.44 No.5
In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of 40°C. The KNU5377 strain evidenced a very similar growth rate at 40°C as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at 43°C. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and H+-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures (43°C), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.
김일섭,Hae Sun Yun,Sun Hye Kwak,진익렬 한국미생물학회 2007 The journal of microbiology Vol.45 No.4
In order to understand the functional role of CPR1 in Saccharomyces cerevisiae KNU5377 with regard to its multi-tolerance characteristics against high temperatures, inorganic acids, and oxidative stress conditions, whole cellular proteins were analyzed via liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This procedure was followed by two-dimensional (2-D) gel electrophoresis. Under menadione stress conditions, the 23 upregulated proteins were clearly identified only in the wildtype strain of KNU5377. Among the proteins, Sod1p, Tsa1p, Ahp1, Cpr1p, Cpr3, Ssb2p, and Hsp12p were identified as components of antioxidant systems or protein-folding related systems. The CPR1 protein could not be completely detected in the cpr1Δ mutant of KNU5377 and the other upregulated proteins in the wild-type strain evidenced a clear correlation with the results of immunoblot analysis. Moreover, a reduction in growth patterns (about 50%) could be observed in the cpr1Δ mutant, as compared with that of the wild-type strain under mild MD stress conditions. These results indicate that the upregulation of CPR1 may contribute to tolerance against MD as an inducer of oxidative stress.
Noncommunicating Spinal Extradural Meningeal Cyst in Thoracolumbar Spine
김일섭,손병철,이상원,홍재택 대한신경외과학회 2010 Journal of Korean neurosurgical society Vol.48 No.12
Spinal extradural meningeal cyst has been rarely reported, whose etiologies are assumed to be the communication of cerebrospinal fluid (CSF)between intradural subarchnoid space and cyst due to the congenital defect in dura mater. Although the CSF communication due to this defect can be found, in most case, few cases in which there is a lack of the communication have also been reported. We report a case of the huge extradural meningeal cyst occurring in the thoracolumbar spine (from T10 to L2) where there was a lack of the communication between the intradural subarachnoid space and cyst in a 46-year-old man who presented with symptoms that were indicative of progressive paraparesis and leg pain. The patient underwent laminectomy and cyst excision. On intraoperative findings, the dura was intact and there was a lack of the communication with intradural subarachnoid space. Immediately after the surgery, weakness and leg pain disappeared shortly.