사람의 골수 줄기 세포로부터의 골세포 분화 과정에서 BMP-2가 미치는 영향과 그에 따른 분화 유전자의 발현 비교 연구

김인숙,장옥련,조태형,이규백,박용두,노인섭,황순정,김명진,이종호,Kim, In-Sook,Zhang, Yu-Lian,Cho, Tae-Hyung,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Hwang, Soon-Jung,Kim, Myung-Jin,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

논문편 : 가정관리능력 및 가정 생활만족도에 따른 농가유형 구분

김인숙,김진호 ( In Sook Kim,Jin Ho Kim ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.6

논문편 : 농가유형별로 본 농가소득 및 소비지출 구조분석 - '88 농가가계부를 중심으로 -

김인숙,최은숙 ( In Sook Kim,Eun Sook Choi ) 대한가정학회 1990 Family and Environment Research Vol.28 No.3

쥐의 골수로부터 추출한 줄기세포를 이용한 조골세포로의 분화 유도과정에서 나타난 문제점에 관한 분석 연구

김인숙,조태형,장옥련,이규백,박용두,노인섭,이종호,김명진,황순정,Kim, In-Sook,Cho, Tae-Hyung,Zhang, Yu-Lian,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Lee, Jong-Ho,Kim, Myung-Jin,Hwang, Soon-Jung 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

This study was aimed to characterize osteogenic potential of rat bone marrow stromal cells (BMSC) isolated with standard flushing method and investigate the plasticity of transdifferentiation between osteoblastic and adipocytic lineage of cultured BMSC. Unlike aspiration method in human, rat bone marrow was extracted by means of irrigation with culture media that elevates the possibility of co-extraction of committed osteoprogenitor, or preosteoblast or other progenitor cells of several types present inside bone marrow. The cultured stromal cells showed high ALP activity which is representative marker of osteoblast without any treatment. Osteogenic inducers such as Dex and BMP-2 were examined for the evaluation of their effect on osteogenic and adipocytic differentiation of stromal cells, because they function as osteoinductive agent in stromal cells, but simultaneously induce adipogenic differentiation. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity or mRNA expression of osteoblast markers such as osteopontin, bone sialoprotein, collagen type I and CbfaI, and in vitro matrix mineralization by von Kossa staining. Oil red staining method was used to detect adipocyte and adipocytic marker, aP2 and $PPAR{\gamma}2$ expression was examined using RT-PCR. It can be supposed that irrigation procedure resulted in high portion of already differentiation-committed osteoprogenitor cell showing elevated ALP activity and strong mineralization only under the supplement of $100{\mu}M$ ascorbic 2-phosphate and 10mM ${\beta}$-glycerophosphate without any treatment of osteogenic inducers such as Dex and BMP-2. Dex and BMP-2 seemed to transdifferentiate osteoprogenitor cells having high ALP activity into adipocytes temporarily, but continuous treatment redifferentiated into osteoblast and developed in vitro matrix mineralization. This property must be considered either in tissue engineering for bone regeneration, or in research of characterization of osteogenic differentiation, with rat BMSC isolated by the standard irrigation method.

토양에서 분리된 Aeromonas hydrophila AM-28이 생산하는 단백질 가수분해효소의 특성

김인숙,김형권,이정기,배경숙,오태광,Kim, In-Sook,Kim, Hyung-Kwoun,Lee, Jung-Kee,Bae, Kyung-Sook,Oh, Tae-Kwang 한국미생물학회 1994 미생물학회지 Vol.32 No.4

토양으로부터 탈지대두박을 잘 분해하는 단백질 가수분해효소를 생산하는 미생물을 분리하여 형태적, 생화학적 특성과 세포벽 지방산조성을 조사한 결과, 분리된 세균은 Aeromonas hydrophila로 동정되었다. 분리된 세균은 glycerol을 탄소원으로, tryptone 및 $(NH_4)_2HPO_4$을 진소원으로, $CaCl_2$를 무기원으로 사용한 배지에서 효소를 가장 많이 생산하였으며, 생산된 효소의 반응최적 pH는 8.0이고, 반응최적온도는 $65^{\circ}C$였다. 이 효소는 $55^{\circ}C$ 이하의 온도에서, pH 7.0에서 13.0의 범위에서 비교적 안정한 것으로 나타났다. 또한 이 효소는 금속이온 $Cu^{2+},\;Hg^{2+}.\;Mn^{2+}$ 및 $Zn^{2+}$에 의해서 효소활성이 저해를 받고, 효소저해제 phenylmethylsulfonyl fluoride (PMSF)와 ethylenediaminetetraacetic acid (EDTA)에 의해서 효소활성이 강력히 저해되는 성질로부터 효소활성부위에 serine기와 금속이온을 갖고 있는 것으로 밝혀졌다. A bacterial strain NO. AM-28, showing proteolytic activity against defatted soybean was isolated from domestic soil. The isolated strain was identified as Aeromonas hydrophila by both the biochemical tests using API kit and the analysis of cellular fatty acid profile with MIDI system. The protease production from A. hydrophila AM-28 was highly enhanced when it was cultivated in the medium containing glycerol as a carbon source, tryptone or $(NH_4)_2HPO_4$ as a nitrogen source, and $CaCl_2$ as a mineral source. The optimal pH and temperature for the enzyme was 8.0 and $65^{\circ}C$, respectively. The enzyme was stable up to $55^{\circ}C$ and at pH values ranging from 7.0 to 13.0. The enzyme activity was inhibited by phenylmethylsulfonyl fluoride and EDTA, indicating that serine residue and metal ions be involved in enzyme activity.

논문편 : 농촌여성 노동의 화폐적 가치평가를 위한 일 연구

김인숙,최은숙 ( In Sook Kim,Eun Sook Choi ) 대한가정학회 1991 Family and Environment Research Vol.29 No.2

한국인의 성인 하악 치조골 결손 형태의 빈도 및 분포에 관한 연구

김인숙,감한평,김종관,Kim, In-Sook,Kim, Han-Pyong,Kim, Chong-Kwan 대한치주과학회 1983 Journal of Periodontal & Implant Science Vol.13 No.1

조직배양에 의한 위도상사화(Lycoris uydoensis M. Kim)의 효율적 기내번식

고정애 ( Jeong Ae Ko ),김인숙 ( In Sook Kim ),정공수 ( Gong Soo Jeong ),최정란 ( Jeong Ran Choi ) 전북대학교 농업과학기술연구소 2012 농업생명과학연구 Vol.43 No.2

위도상사화(Lycoris uydoensis M. Kim)의 효율적인 기내 급속 대량 증식체계를 확립시키고자 잎과 인편을 배양하였다. 5℃에서 60일간 저온 처리한 모구를 구고를 중심으로 상부, 중부 및 하부로 구분하여 0.1 mg·L-1 2,4-D와 1.0 mg·L-1 BA, 0.5 mg·L-1 NAA와 1.0 mg·L-1 BA 또는 3.0mg·L-1 IBA와 3.0 mg·L-1 BA 혼용처리된 MS 배지에 배양한 결과 식물생장조절물질과는 관계없이 디스크가 부착된 인편의 하부가 캘러스 및 자구 형성에 가장 효과적인 부위였다. 캘러스는 잎절편이나 인편조직에서 쉽게 형성되었으나 소자구 및 신초는 인편에서만 형성되어 기내번식에 적합한 절편체는 인편이었다. 자구 형성 및 신초분화는 배지내 첨가된 식물생장조절물질에 따라 치상체에서 직접 자구를 형성하거나 캘러스를 통해 식물체로 분화되는 2가지 경로로 형성되었는데 디스크가 부착된 인편을 0.1 mg·L-1 2,4-D와 1.0 mg·L-1 BA, 0.5 mg·L-1 NAA와 1.0 mg·L-1 BA 또는 3.0 mg·L-1 IBA와 3.0 mg·L-1 BA 혼용처리된 MS 배지에 배양하여 30일 이내에 60%-100%까지 유도하였다. 다량의 자구형성 및 유지는 MS배지에 0.1 mg·L-1 2,4-D와 1.0 mg·L-1 BA가 혼용되거나 또는 3.0 mg·L-1 IBA와 3.0mg·L-1 BA 혼용처리 되었을 때 0.5 mg·L-1 NAA와 1.0mg·L-1 BA 혼용처리 되었을 때 보다 효과적이었다. 6% sucrose는 자구형성 및 비대를 촉진시켰으며 특히 3.0 mg·L-1 IBA와 3.0 mg·L-1 BA 혼용처리배지에 6% sucrose를 첨가시키므로 0.1 mg·L-1 2,4-D와 1.0 mg·L-1 BA가 혼용 배지보다 구의 비대가 1-2배 증가하였다. 인편유래 캘러스로부터 기관분화 또는 인편에서 직접 분화된 자구에서 성공적으로 식물체가 재분화되었다. This study was conducted to establish an effective in vitro mass propagation system using leaf and bulb scale explant sources of Lycoris uydoensis M. Kim. For the bulb scale culture, the bulbs treated at 5℃ for 60 days were dissected into three parts according to bulb height (upper, middle, and bottom), and allowed for a culture on the MS medium with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA plus 3.0 mg ? L-1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. Callus was readily produced on the young leaf segment and bulb scale tissues, whereas, bulblets and shoots were formed only on bulb scale explants. Bulb scales with disk appeared to the best optimal explant for in vitro propagation. Two distinct pathways, either organogenesis through callus or direct bulblet formation, could be recognized in bulb scale culture. Bulblet formation and shoot organogenesis from the bulb scales with disk were effectively induced up to the 60%~100% on the MS medium supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA within 30 days of culture. On MS media supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA or 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA were more effective than 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA for induced and maintained a large number of bulblets. Six percent of sucrose enhanced bulblet formation and expanded the bulblets. Especially, adding 6% of sucrose on MS media supplemented with 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA was one or two times of bulblets expanted than on MS mediun with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA plus 6% of sucrose. Plantlet regeneration was successfully achieved from bulb scale-derived callus, via shoot bud induction or direct bulblet formation.

일개 도시지역 임신부의 영양섭취에 관한 조사연구 -간이식 영양조사법 이용-

김인숙,Kim, In-Sook 대한예방의학회 1983 Journal of Preventive Medicine and Public Health Vol.16 No.1

Aiming at the total 200 pregnant women including 150 ones registered with the Health Center of Chung Ku District and 50 ones registered with the Health Center of Mapo District, we have conducted a research study of the socio-medical characteristics, maternal child health, and the status of nutritional intake which has utilized the application of convenient method for the study of nutritional status, during the period of April 20 to May 25, 1983. And we have obtained the following conclusions: 1. Regarding age distribution, the pregnant women aged from 26 to 30 were most numerous, which was 54.0%. The percentage of women who experienced the first pregnancy was 12.0% and the percentage of those who experienced the second pregnancy was 37.0%, which was the highest. Regarding the weeks of pregnancy of the pregnant women, the first trimesterr was 11.5%, the second trimester 30.0%, and the third trimester 58.6%. 2. Regarding academic achievements, the pregnant women who graduated from middle schools reached 43.5%, which was the highest percentage. Regarding economic status, the pregnant women who owned their own houses were only 21.0%. And the pregnant women whose monthly income was from 300,000 won to 400,000 won were 40%, which was the most numerous. 5. The women above 15 years old who experienced the first menstruation were 84.0%. And those who experienced abortion were totally 54.4%. and 35.5% among those women experienced artificial abortion. 4. 70.5% of the pregnant women said that their health condition was excellent, 24.5% felt subjective complaints, and 5.0% specially received medical consultation for their diseases. 5. 82.0% received prenatal care, but 60.5% regularly received prenatal care. 68.0% received the education for nutrition and only 19.5% regularly received the education for nutrition. 6. Regarding the family composition, the families consisting of two generations were 47.0%, which was the most numerous. 97.5% of the preparation for meals was conducted by housewives. They said that they did not lack time for meal preparation. 7. 94.9% of the pregnant women said that they had eaten as in ordinary times during their pregnancy. 25.5% said that there were tabooed foods. Tabooed foods are chiefly pork, chicken, milk, and eggs. 68.0% don't drink milk during pregnancy, 32.5% take the intake of vitamins, and 20.5% take iron supplement. 8. The average amounts of the intake of protein, fat, and carbohydrate of a pregnant women are 49.3gm, 29.4gm, and 205.1gm respectively, which showed the phenomenon in which the amount of the intake of nutrition increased as the weeks of pregnancy increased. The average amount of the intake of salt was 14.2gm. 9. Regarding the hemoglobin value of all pregnant women, those whose hemoglobin value was less than 11.0gm were 66.5%, those whose hemoglobin value was 11 to 12 gm were 16.5%. and those whose hemoglobin value was above 12gm were 17.0%. The pregnant women whose hemoglobin value was less than 11.0gm in the first trimester of pregnancy, in the second trimester, and in the trimester were 81.8%, 62.8%, and 64.9% respectively. This shows that the phenomenon of anemia increased as the weeks of pregnancy increased 10. Regarding physical development of a pregnant woman during prenatal period, the height and weight were $156.7cm{\pm}14.1$ and $51.1kg{\pm}58$ respectively. When the standard increase of a prenatal weigt gain is set as 100%, the women over the range of 100% were 28.0% and the women under the range of 80% were 37.0%.

세침 천자 검사로 진단된 유방종양의 세포병리학적 연구

김인숙,이중달,Kim, In-Sook,Lee, Jung-Dal 대한세포병리학회 1990 대한세포병리학회지 Vol.1 No.1

Fine needle aspiration biopsy cytology (FNA) for diagnosis of a variety of breast tumors has been proven to be a simple, safe, and cost saving diagnostic methodology with high accuracy. Cytologic specimens from 1,029 fine needle aspirations of the breast during last 3-year period were reviewed and subsequent biopsies from 107 breast lesions were reevaluated for cytohistological correlation. FNA had a sensitivity of 81.6% and a specificity of 98.3%. One oui of 107 cases biopsied revealed a false positive result (0.9%) and the case was due to misinterpretation of apocrine metaplastic cells in necrotic backgound as malignant cells. A false negative rate was 8.4% (9 of 107 cases biopsied). Six of 9 false negative cases were resulted from insufficient aspirates for diagnosis, and remaining three of 9 false negative cases revealed extensive necrosis with no or scanty viable cells on smears. The results indicate that for reducing false positive and false negative rates of FNA, an experienced cytopathologist and a proficient aspirator are of great importance.