건강인의 백혈구 백분율에 대한 검색

김영활 ( Young Hwal Kim ),조창호 ( Chang Ho Cho ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

To evaluate reference intervals of the leukocyte differential count in healthy persons, we measured WBC and five leukocyte differential count, determined by Coulter Counter model GEN-S in 1,060(M: 495, F: 565) persons. The results were summarized as follows 1. The correlation between Coulter GEN-S and the manual leukocyte differential shows good correlation for neutrophils(r=O.88), lymphocytes(r=O.76), monocytes(r=O.52) and eosinophils(r=O.89). π1e correlation for basophils (r=O.18) was less acceptable. 2. Reference interval of WBC count : 3.5-9.9 x 103/mm``(Male: 4.l-1O.5, Female: 3.6-9.2). 3. Reference interval of neutrophils ; 37.2-7 1.2%(Male: 38.0-7 1.2, Female: 36.5-7 1.3). 4. Reference interval of lymphocytes ; 20.5-52.5 % (Male: 19.9-5 1.1, Female: 21.4-53.4). 5. Reference interval of monocytes ; 3.2-9.6%(Male: 3.2-10.0, Female: 3.0-9.4). 6. Reference interval of eosinophils ; 0.0-5.9%(Male: 0.0-6.5, Female: 0.0-5.4). 7. Reference interval of basophils ; 0.0- 1.4%(Male: 0.0- 1.1, Female: 0.0- 1.7)

심전도 이상군과 대조군에 었어서 이완기 및 수축기 혈압과 총콜레스테롤과의 관계

김병원 ( Byung Weon Kim ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

We have examined the blood pressure and the value of total cholesterol of 2,438 person visited on Soonchunhyang university hospital in Kumi for the hea1th examination from Jauary to December in 1995. The patient that was shown abnormal ECG was 317 person (13%) of the hea1th examined person (2,438) and in the abnormal ECG patient, male were 199 person (19.2%) and female were 118 person (9.9%), and the emerging ratio ofabnormal ECG was shown 24.1 % in 60 ages, 19.6% in 50 ages and 9.9% in 40 ages eκ.Also we have analyzed the blood pressure and the value of total ch이esterol from the normal electrocardiogram group (317 person) , control group and the abnormal ECG group (317 person) which were selected randomly. πle resu1t was followed as below.1. In the normal ECG group, the diastolic and systolic blood pressure and the value of total cholesterol was 78.61 i= 16.51 mmHg and 119.38 i= 10.56 mmHg, and 187.23 i= 14.52 mgjdl respectively but in the abnormal ECG group, 83.89 i= 13.34 mmHg and 131.98 i= 21.09 mmHg, and 187.23 i= 14.52 mgjdl respectively. 2. In the abnormal ECG group, the average of the diastolic blood pressure was high (94.03 i= 12.20 mmHg) in the left venπicular hypertrophy (L VH) and total cholester,이 195.24 i= 35.41 mg/dl in the ischemia. In the normal and abnormal ECG group, over 100 mmHg in the diastolic blood pressure was 11 and 57 person, over 160 mmHg in the diastolic blood pressure was 4 and 40 person, and over 250 mg/dl in the total cholesterol was 6 and 20 person respectively. 3. In the correlation analysis, the correlation of the diastolic and systolic blood pressure, and the total cholesterol was r=0.10 ψ<0.05), r=O. lO ψ<0.05) and r=0.12 (p<0.05) respectively. From these results, we knew that the emerging ratio of abnormal ECG was high according to increasing ages (50-60 ages) , and the diastolic and systolic blool prelusure and the value of total cholesterol was increased correlatively in the abnormal ECG group than normal ECG group

알츠하이머병 환자에서 콜린에스터라제 억제제의 투여에 의한 대뇌피질 국소혈류의 변화

문성식 ( Seung Sik Moon ),김영활 ( Young Hwal Kim ),김병원 ( Byung Weon Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.1

Alzheimer`s Disease(AD) is a progressive, degenerative disease that attacks the brain resulting in impaired memory, thought processing, and behavior. This is associated with a loss in presynaptic cholinergic function. It has been suggested that cholinergic inhibitors could restore this function and improve some symptoms of AD. Previous studies have shown that cholinesterase inhibitors(ChEI) improve cognitive and global functions in patients with mild to moderate AD. This study aims to evaluate regional cerebral blood flow(rCBF) changes and contemporary clinical responses such as cognitive and psychiatric symptoms after ChEI treatment. The subjects were eight at risk AD patients(four males and four females, mean age 69.63 years) recruited from the department of Neurology at Yeungnam University Medical Center between August 2000 and April 2002. The clinical diagnosis of AD was based on DSM-Ⅳ and NINCDS-ADRDA criteria. Huchinski ischemic scores of all the patients were below 1. The mean treatment duration was 30.38 weeks, ranging between 24 to 44 weeks. Four patients received Rivastigmine(Exelon®) 12mg after titration, three patients received Donepezil(Aricept®) 10mg during the whole period, and one patient received Donepezil 10mg after the initial 5mg for three weeks. The base line and follow up Ethylene Cysteine Diethylester Single Photon Emission Computed Tomography(99mTC-ECD SPECT) studies were done within one week prior to ChEI treatment and within one week following the study. Regions of interest(ROIs) were drawn over the left and right frontal lobe, temporal lobe, parietal lobe, and cerebellum. Region to cerebellar ratio(RCR)(each count in the ROIs divided by mean cerebellar ROI count) were calculated as a estimation of rCBF, and base line RCRs were compared with those of the follow up. The results display an overall increase in global cerebral blood flow through K-MMSE(Korean-Mini Mental State Examination), CDR(Clinical Dementia Rating), CDR-SB(Clinical Dementia Rating-Sum of Box), GDS(Global Deterioration Scale), and NPI(Neuropsychiatric Inventory, Cummings). We found that the most significant increase in blood flow occurred in the bilateral parietal lobes(p<0.05). Reduction in the rCBF is more profound on the left hemisphere in the base line(p<0.05) where there is a significant increase of rCBF after ChEI treatment compared with to right hemisphere(p<0.05).

에어로빅스가 청각유발전위에 미치는 영향

박상남 ( Sang Nam Park ),김영활 ( Young Hwal Kim ),김병원 ( Byung Weon Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.3

Up to now, there have been rare clinical studies on leaders and aerobics athletes. To get the useful data for protecting from auditory disorder, we selected 15 female aerobics leaders (experimental group) and 15 females (control group) unexperienced in aerobics and a without neurological and octolaryngological disorder. The average age was 34.87±8.80 (experimental group) and 34.07±8.45 (control group) years, and the average career of an aerobics leader (experimental group) was 8.33±4.73 years. We measured the auditory evoked potential (AEP) of the two groups treated with 70, 75 and 85 dB intensity from January 2006 to May 2006 and analyzed the absolute latency (AL) and interpeak latency (IPL) by the SPSS/pc+ 12.0 program. In the comparison of the AL between the experimental group and the control group according to intensity, both ears treated with 70 and 75 dB had a significant difference (p<0.05) in the Ⅰ, Ⅲ, Ⅴ wave and in theⅠ, Ⅴ wave respectively, and the experimental group treated with 85 dB showed a difference in the Ⅰ, Ⅲ, Ⅴ wave (left ear) and in theⅠ wave (right ear) respectively. The IPL of the two groups treated with various intensities had no prolongation. In the comparison of the AL between the experimental group and the control group according to ages, the experimental group in their 20s treated with 70 dB showed a significant difference (p<0.05) in the Ⅴ wave (left ear) and in the Ⅰ, Ⅲ, Ⅴ wave (right ear), and the experimental group in their 20s treated with 75 dB in theⅠ, Ⅲ wave (left ear) and in Ⅰ, Ⅲ, Ⅴ wave (right ear), and experimental group in their 20s treated with 85 dB in the Ⅴ wave (left ear) and in the Ⅲ, Ⅴ wave (right ear). The experimental group in their 30s treated with 70 dB had a significant difference (p<0.05) only in the Ⅴ wave (right ear). Only in the IPL of subjects in their 20s treated with 85 dB, Ⅲ-Ⅴ and Ⅰ-Ⅴ of both ears was extended. In the comparison of the AL and IPL according to career, there was no significant difference between the two groups. From this results, we concluded that the lower sound intensity (70 dB) showed a more significant difference in the experimental group than the control group. We concluded that the leader of aerobics exposed to louder sounds than normal people are affected by auditory neurological and octolaryngological disorders. So we think that the leaders of aerobics need to control the noise level for protecting themselves against a disease.

체감각 유발전위검사를 이용한 정상 성인의 외측대퇴피부신경의 잠복기와 진폭의 참고치 추정

문성식 ( Seung Sik Moon ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

Meralgia paresthetica(MP) which is characterized by paresthesias and sensory impainnent without motor weakness in the anterolateral aspects of the thigh is produced by compression of the lateral femoral cutaneous nerve(LFCN). Even though the diagnosis of MP is mostly based on the clinical symptoms, electrophysiologic study is mandatory to confmn the disease o비ectively. It has been known that somatosensory evoked potential(SSEP) study of LFCN is a simple and very useful method to evaluate MP, so we studied SSEP of LFCN in normal adults and offer normal values. 36 normal adults(M:F=23:13) ages from 21 to 73 years 01d(mean土 SD:42.1土15.7) were studied SSEP of LFCN bilaterally. The stimulation site was anterolateral in aspect of thighs and the recording site was Cz’. πle mean values(msec ::t SD) of LPO, SPO, LN1 and SN1 of all subjects were 35. 1O土t 2.42, 33.80土2.4, 43.68 土1.88 and 42.l6 ::t 2.l2 and the mean values(msec ::t SD) of DPO, DNl and DA were 1.30 ::t 1. 14, 1.52土t 1.38 and 0.32土t O.33. For the diagnosis of MP, comparison of latency difference between both sides is more reliable than simple value of latency it self because of individual differences of body types. According to our results, the latency difference should be less than 2msec and the amplitude difference was less than 60% in normal adults.

성별 및 연령에 따른 안면신경마비 출현 양상에 관한 연구

황경희 ( Kyung Hee Hwang ),김병원 ( Byung Weon Kim ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2

Facial palsy is a general nerve disease which is occurred in a lateral incomplete or complete facial palsy. But it makes man restrict a social activity and so it is very important that we know the prognosis factors of a patient for medical care. We want to investigate the position and the patterns of the facial palsy according to sex and age. In this study, we performed the excitability test of the facial nerve on 103 patients (male 45 and female 58) which diagnosed as the Bell’s palsy having an acute facial palsy, and the rate of the facial palsy was 42% (43 case) in right oculi and 58% (60 case) in left oculi. The facial palsy was appeared widely from young to elderly patients and especially, the rate of the facial palsy was high in age groups from 40 to 50.

연령과 성별의 차이가 전정 유발근전위에 미치는 영향

문성식 ( Sung Sik Moon ),김경미 ( Kyoung Mi Kim ),김영지 ( Young Ji Kim ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.2

The aim of this study was to examine the effect of differences in age and sex on vestibular evoked myogenic potentials (VEMP), particularly in normal participants. Briefly, the VEMP is a response elicited by loud clicks or tone bursts recorded from the tonically contracted sternocleidomastoid muscle. A total of 72 participants were divided into 6 groups according to their age and sex (20~30/F, 20~30/M, 40~50/F, 40~50/M, ≥60/F, ≥60/M). We got the data of latency, amplitude, and asymmetry index of the amplitude(ASI) from them. As a result of this study, there are variations in VEMP amplitudes and ASI depending on the muscle tension and the intensity of stimuli. In contrast, the latency of the response is usually less varied and does not differ significantly.

단백질 분해효소에 의한 참깨박 단백질의 기능성 변화

이선호(Seon-Ho Lee),조영제(Young-Je Cho),천성숙(Sung-Sook Chun),김영활(Young-Hwal Kim),최청(Cheong Choi) 한국식품과학회 1995 한국식품과학회지 Vol.27 No.5

Phytase 처리에 의한 폐단백자원의 단백질 용출 및 기능성 변화

천성숙(Sung-Sook Chun),조영제(Young-Je Cho),김영활(Young-Hwal Kim),우희섭(Hi-Seob Woo),최청(Chung Choi) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.1

폐단백질을 활용하는 방도의 하나로 폐단백질자원으로부터 토양에서 분리한 Aspergillus sp. 균주가 생산하는 phytase를 이용하여 불용성 단백질의 분리 효율성을 높였으며 추출 단백질의 기능성을 살펴보았다. 참깨박에 함유되어 있는 불용성 형태의 단백질을 가용성 형태의 단백질로 용출시키기 위한 최적 pH, 최적 온도, 최적 처리시간과 최적 첨가효소량은 4.0~5.0, 50℃, 8~10시간, 120unit였고, 효소처리된 참깨박은 phytase 처리의 경우 대조구에 비해 기포형성력은 크게 증가하지 않았으나 기포안정성은 다소 증가하였다. 참깨박 단백질의 유화력은 다소의 유화력과 안정성의 증가가 관찰되었고, 유지흡착력과 수분흡착력은 대조구에 비해서 높은 값을 나타내었다. This study was performed to improve extraction of insoluble proteins and to evaluate funtional properties of abolished proteins by the phytase produced by Asporgillus sp. The optimum pH, temperature, treatment time and unit of the enzyme for extraction of protein were pH 4.0~5.0, 50℃, 8~10hrs and 120 units. The foaming capacity and foaming stability of sesame meal protein after enzyme treatment were virtually unchanged as compared to control. The emulsion capacity and emulsion stability of sesame meal protein was higher than control. Oil absorption as well as water absorption capacities of sesame meal protein were higher than control.

정도관리 개선활동을 통한 파레토 분석

장상우 ( Sang Wu Chang ),서한익 ( Han Ik Sur ),김대식 ( Dae Sik Kim ),김양호 ( Yang Ho Kim ),김영활 ( Young Hwal Kim ),여영규 ( Young Gyu Yeo ),장순모 ( Soon Mo Chang ),민재기 ( Jae Gi Min ),조영주 ( Young Ju Cho ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

Pareto ana1ysis through qua1ity improvement activities is the study of related subjects to determine if one is more significant than the others and used to identify the most important item in group of items. The Pareto chart breaks down an item into its component parts and then arranges and displays those parts in order of importance. Pareto ana1ysis is used to focus problem-solving activities, so that the area causing the most difficulty is addressed first. We eva1uated error problems in c1inica1 chemistry laboratory from J anuary 1997 to December 1998. We used systems thinking for cause and effect diagram for problem-solving and Pareto analysis to solve the problem factors of ana1ytica1 performance of Hitachi 7150 system for two years. We found that tota1 problems reduced 33.5 percentage during one year after educating system thinking, cause and effect analysis. We conc1uded that 8 1. 19 percentage of the total problems controlled by 23% of the items that are two factors. Upon examination, this 80/20 rule(Pareto distribution) proved true in our working area. We use the Pareto distribution to achive an 80% qualiη improvement by working 20% of the problem causes. We think Pareto an떠ysis is a tool for problem-solving during quality improvement activities for total quality management in medical laboratory science.