신증후출혈열 혈청진단법, 간접면역형광항체법과 ELISA법 비교

김동휘,주용규,송진원,백락주,이호왕 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

An ELISA test using suckling rat brain Hantaan virus antigen was developed and its clinical efficacy for the diagnosis of HFRS was compared wlth indirect IF antibody technique. The results were as follows : 1. Higher titer of Hantaan virus antigen was obtained from suckling rat brains than vero E6 cell culture of Baculovirus expressed Hantaan virus nucleocapsidprotein. 2. Antigens for ELISA test made from Hantaan or Seoul viruses cross reacted with sera from HFRS and could not differentiate serologic type of hantavirus infection. 3. During the early stage of HFRS high titer of IgM antibody was detected by ELISA while IgG antibody of high titer was demonstrated by indirect IF antibody technique. The titer of IgM antibody detected by ELISA was about 100 times higher than that by IF. IgG antibody by ELISA increased progressively by 1-2 months and remained in high titer until 5 months after onset of illness. 4. During the course of HFRS, IF technique detected IgM antibody for 2 months and IgG antibody of high titier until 5 months after onset. Althouh IgM and IgG antibody demonstrated by ELISA reached a peak at 1-2 weeks and at 1-2 months respectively, and both antibodies remained in high titer until 5 months after onset. The couclusion is that large amount of antigen for ELISA can be made easily from suckling rat brains and demonstration of IgM antibody against Hantaan virus by ELISA is diagnostic of HFRS.

기술의 해외 진출을 위한 제언

김동휘 한국기술사회 1971 技術士 Vol.4 No.13

External Forces Induced Supramolecular Chirality from Achiral Molecules at Different Dimensions

김동휘,김태형,김상율 한국고분자학회 2022 한국고분자학회 학술대회 연구논문 초록집 Vol.47 No.2

Differentially Expressed Proteins in Nitric Oxide-Stimulated NIH/3T3 Fibroblasts: Implications for Inhibiting Cancer Development

김동휘,김혜영,임주원 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.2

Purpose: Recent evidence shows that nitric oxide (NO) may exhibit both pro-cancer and anti-cancer activities. The present study aimed to determine the differentially expressedproteins in NO-treated NIH/3T3 fibroblasts in order to investigate whether NO induces proteins with pro-cancer or anti-cancer effects. Materials and Methods:The cells were treated with 300 μM of an NO donor 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18) for 12 h. The changed protein patterns, which were separated by two-dimensional electrophoresis using pH gradients of 4‒7, were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Results: Seventeendifferentially expressed proteins were identified in NOC-18-treated cells. Nine proteins [vinculin protein, keratin 19, ubiquitous tropomodulin, F-actin capping protein (α1 subunit), tropomyosin 3, 26S proteasome-associated pad1 homolog, T-complex protein 1 (ε subunit) NG-dimethylarginine dimethylaminohydrolase, and heat shock protein 90] were increased and eight proteins (heat shock protein 70, glucosidaseII, lamin B1, calreticulin, nucleophosmin 1, microtubule-associated protein retinitis pigmentosa/end binding family member 1, 150 kD oxygen-regulated protein precursor, and heat shock 70-related protein albino or pale green 2) were decreased by NOC-18 in the cells. Thirteen proteins are related to the suppression of cancer cell proliferation, invasion, and metastasis while two proteins (heat shock protein 90 and NG-dimethylarginine dimethylaminohydrolase) are related to carcinogenesis. The functions of 150 kD oxygen-regulated protein precursor and T-complex protein 1 (ε subunit) are unknown in relation to carcinogenesis. Conclusion: Most proteins differentiallyexpressed by NOC-18 are involved in inhibiting cancer development.

Solitary Piloleiomyoma in the Scalp

김동휘,이지성,김진아,이중호 대한두개안면성형외과학회 2017 Archives of Craniofacial Surgery Vol.18 No.1

Cutaneous leiomyomas can be classified into three types according to the site of origin: piloleiomyoma, angioleiomyoma, and dartoic (genital) leiomyoma. It might be expected that leiomyomas are commonly found on the scalp because there are many arrector pili muscles and vessels. However, leiomyomas are actually rarely reported in the scalp. Recently, we observed a case of cutaneous leiomyoma in the scalp and present our experience along with a literature review. Keywords:

Correlation between meniscal extrusion and symptom duration, alignment, and arthritic changes in medial meniscus posterior root tear: research article

김동휘,이광철,Hyun Hak Kim,Dong Hyuk Cha 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Background: Medial meniscus posterior root tear can result in medial meniscus extrusion. However, the severity of medial meniscus extrusion is different in each root tear patient. The purpose of this study was to identify the factors that contribute to the severity of medial meniscus extrusion with medial meniscus posterior root tear, such as duration of disease, the degree of arthritis—chondral wear, subchondral edema, osteophyte size, and Kellgren–Lawrence (K/L) grade—and mechanical alignment for appropriate treatment method. Methods: From January 2009 to August 2014, we retrospectively analyzed magnetic resonance imaging (MRI) and simple x-ray of 99 patients with medial meniscus posterior root tear. The duration of the disease was identified through retrospective chart review. The severity of medial meniscus extrusion, the presence of subchondral edema, the degree of chondral wear, and the size of the osteophyte were measured on MRI. K/L grade was confirmed on simple x-ray, and the mechanical axis was measured on whole extremity radiographs. Statistical analysis was performed by using bivariate correlation analysis and one-way analysis of variance. Results: The mean medial meniscus extrusion was 4.61mm, and the mean duration of the disease was 15.52 months. The mean degree of chondral wear was 25.8%, and 63 out of 99 cases showed subchondral edema. The average alignment was 4.30 degrees, and the average size of the osteophyte was 1.48mm. There were 40 cases (40.4%) with K/ L grade I, 48 cases (48.5%) with grade II, 11 cases (11.1%) with grade III, and no cases with grade IV. In the group mean analysis between the K/L grade and the severity of medial meniscus extrusion, the average medial meniscus extrusions were 3.97mm in grade I, 4.93mm in grade II, and 5.59mm in grade III. There was a statistical significance between the size of the osteophyte and the severity of medial meniscus extrusion (P = 0.000), K/L grade, and the severity of medial meniscus extrusion (P = 0.001). Conclusions: The severity of medial meniscus extrusion with medial meniscus posterior horn root tear is associated with the size of the osteophyte and K/L grade.

Aerosol Mass Spectrometer (AMS)-Based Real-Time Physicochemical Characterization of Atmospheric Aerosols

김동휘 사단법인 한국질량분석학회 2022 Mass spectrometry letters Vol.13 No.2

Atmospheric aerosols have become a major environmental concern because of their adverse effects on human health, air quality, and climate change. Over the last few decades, several mass spectrometry (MS)-based techniques have been devel- oped and applied in the field of atmospheric aerosol research. Particularly, real-time measurement of ambient aerosols using an aerosol mass spectrometer (AMS) has become one of the most powerful tools for aerosol chemistry. This review provides a brief description of AMS and its applications for understanding the physicochemical properties of atmospheric aerosols, as well as its sources and evolution processes.

좌관상동맥 주간부와 우관상동에서 기원하는 이중 좌전하행동맥

김동휘,문건웅,김은희,우기현,신진경,장지연,하성은,이주영 영남대학교 의과대학 2014 Yeungnam University Journal of Medicine Vol.31 No.1

Congenital abnormalities of the coronary arteries are found in 0.6% to 1.3% of patients in coronary angiography. Dual left anterior descending coronary artery (LAD) is a rare coronary anomaly and is incidentally detected during coronary angiography. We report a case of a 65-year-old female with a rare coronary anomaly who was diagnosed with dual LAD via coronary computed tomography and coronary angiography. The imaging studies revealed dual LAD originating from the left main stem and right coronary sinus. These angiographic findings were considered to be consistent with the type IV variety of dual LAD by Spindola-Franco classification. Recognition of dual LAD is important to prevent errors of interpretation of the coronary angiogram and for optimal surgery.

Supramolecular Chirality of Triphenylamine Derivatives Having Multiple Hydrogen Bondings

김동휘,김상율,정성욱 한국고분자학회 2020 한국고분자학회 학술대회 연구논문 초록집 Vol.45 No.2

정중신경의 검사 길이가 수근관 증후군 진단에 미치는 영향

김동휘,강윤규,황미령 대한재활의학회 2004 Annals of Rehabilitation Medicine Vol.28 No.1

Objective: To evaluate the influence of the length of the median nerve in the diagnosis of carpal tunnel syndrome (CTS). Method: Inching test of median sensory nerve using TenElectrodes was performed in 114 hands of 68 CTS patients and 68 hands of 34 controls. The abnormal cut-off values of 1-cm, 2-cm, 3-cm, 4-cm, 5-cm, 6-cm, and 7-cm segment studies were calculated as the maximal conduction delay per centimeter (maximal CD/cm)+SD×2 for each segment in controls. Based on such values, the sensitivity of each segment study in the CTS group and the specificity in controls were obtained. Results: In controls, mean CD/cm was 0.20±0.08 ms, and maximal CD/cm was 0.26±0.08 ms in the segment between 2 and 3 cm distal to distal wrist crease. With the abnormal cut-off value set at 0.26 ms, the sensitivity of the 1-cm segment study was 98.5% in CTS. The sensitivities of the 2-cm through 7-cm segment studies were 93.9%, 93.9%, 92.4%, 92.4%, 92.4%, and 90.9%, respectively. Conclusion: These suggest that studies of longer nerve segments tend to lower the sensitivity of the test because the inclusion of the unaffected segments in calculation attenuates the effect of conduction delay at the site of the lesion.