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- 저자 : 고유석 ( Tae Young Ahn (검색결과 5 건)
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대호 (大湖) 저수지 (貯水池) 퇴적토의 (堆積土) 탈질화 (脫窒化) 세균과 탈질화 (脫窒化) 작용능에 미치는 환경요인
고유석,안태영 ( You Seak Go,Tae Young Ahn ) 한국하천호수학회 1992 생태와 환경 Vol.25 No.3
The denitrifying potential and various environmental factors were investigated and the influence of environmental factors on the distribution of denitrifying bacteria and potential was analyzed through stepwise multiple regression in the sediment of lake Daeho. The N_2O production was measured with gas chromatography at in situ temperature by acetylene blockage method and the N_2O production rates were 0∼9.18㎍-N/g-dw/day. The genera that showed denitrification potential were identified as Pseudomonas, Corynebacterium, Flavobacterium, etc. It was shown through statistical analysis that the environmental factors affecting the variation of denitrifying bacteria and potential differed with stations. NO_3 was used as a terminal electron acceptor, however terminal electron acceptor could be changed by environmental factors.
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이일규,고유석,한석균,안태영 ( Il Gyu Lee,You Seok Go,Suk Kyun Han,Tae Young Ahn ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.1
Limiting factors for the nitrogen fixation potentials by heterotrophic bacteria are not ammonia but carbon and phosphate. The nitrogen fixation potentials were investigated by acetylene reduction method that was added carbon, phosphate, and ions. The nitrogen fixation potentials ranged 0∼520.6μM-ethylene/L/day in Lake Soyang and 0∼615.3μM-ethylene/L/day in Daechung Reservoir. The nitrogen fixation potentials showed positive correlation with water temperature in Lake Soyang (r=0.75, P<0.01) and Daechung reservoir (r=0.71, P<0.01). As a result, the nitrogen fixation potential by heterotrophic bacteria were mainly affected by water temperature in Lake Soyang and Daechung reservoir.
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전은형,고유석,한석균,안태영 ( Eun Hyoung Jeon,You Seak Go,Suk Kyun Han,Tae Young Ahn ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.4
From September 1992 to August 1993, nitrogen assimilation and mineralization along with selected biological and physico-chemical environmental factors were investigated 9 times at two different stations in Lake Soyang. For the analysis of assimilation and mineralization, ion concentration analysis method was used. The range of nitrogen assimilation and mineralization rates were 0-100.6㎍-N L^-1 day^-1 and 0∼130.3㎍.-N L^-1 day^-1·respectively. At station 1. where was influent area, assimilation rate which was measured in the surface was correlated positively with temperature (r=0.82. p<0.01) and variation of ammonia (r=0.70, p<0.05) and it was correlated with chlorophyll-a (r=0.87, p<0.01) and nitrate (r= -0.75, p<0.05) in bottom against surface. At station 2, where was Joined with main and influent stream, assimilation rate in surface was correlated with temperature(r=0.77, p<0.05), pH (r=0.70,p<0.05) and ammonia (r=0.69, P<0.05) and mineralization rate in surface was positively correlated with temperature(r=0.86, p<0.01)and pH (r=0.84, p<0.01). Mineralization rate in bottom was correlated with N/P ratio (r=-0.67, p<0.05).
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안태영,곽노태,고유석 ( Tae Young Ahn,No Tae Kwak,You Seak Go ) 한국환경생물학회 1992 환경생물 : 환경생물학회지 Vol.10 No.2
From April 1990 to March 1991, nineteen variables of physico-chemical factors, bacteria and activity were investigated 12 times at the 3 stations in Lake Daeho and the influence of environmental factors on the distribution of bacteria and activity were analysed through stepwise multiple regression. The results of multiple regression were as follows. At station 1, the variation of bacterial populations and activity was controlled by that of available nitrogen, ammonia and nitrate. At station 2, water temperature and BOD were shown as the major factors affecting the variation of microbial populations. The variation of heterotrophic bacteria and spore forming bacteria could be controlled by the variation of BOD and water temperature could explain the variation of proteolytic and lipolytic bacteria. The variation of total coliform bacteria depended on the variation of pH and nitrate by 68%. Fifty five percent of the variation of glucose turn-over rate was affected by phospate and salinity. At station 3, dissolved oxygen was found out as a main factor governing the variation of bacterial populations. The variations of heterotropic, proteolytic and lipolytic bacteria depended on dissolved oxygen by 32%, 32%, 58% and 59% repectively. Lipolytic bacteria was affected by water temperature as same as at station 2. And glucose turnover rate had a close relation to chlorophyll a.
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가축분뇨를 이용한 SCP 생산 균주의 분리 및 균체 단백질 생산
한석균,고유석,안태영,배동훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6
질소원으로서 계분을 이용하는 균주를 선별하고 계분배지에서 균체의 생육속도가 다른 균주에 비하여 우수한 균주를 분리하였다. 형태·생리학적 특성을 기초로 하여 yeast의 분류 기준과 비교하여 본 균주를 Candida sp.로 동정하였으며 본 균주를 Candida sp. D116으로 명명하였다. Poultry feces extract medium에서 4% 농도의 glucose 첨가가 균체 생육에 효과적이었다. D116 균주를 액체 발효하여 균체생산능, 요산 그리고 가용성 단백질의 변화를 조사하였다. 그 결과 약 60시간이 경과하면 액체 발효 배지내의 거의 모든 가용성 단백질 및 요산의 감소를 보였으며 균체생육은 약 36시간 배양하였을 때 최고조에 도달하였고 그 후에는 점차 감소하는 경향을 보였다. SCP의 대량생산의 결과 50%의 계분혼합 배지와 30℃의 배양 온도에서 36시간 배양하여 균주의 생육수준이 3.8×10^9 CFU/ml 농도의 균체를 생산하였고 200 L의 배양액중 약 870 g-dw의 균체를 얻었으며 생산된 군체의 조단백질 함량은 67%이었다. Production of Single Cell Protein from Poultry Feces. Suk-kyun Han, You-Suk Go, Tae-Young Ahn and Dong-Hoon Bal^1*. Deparment of Microbioligy, College of Natural Sciences, Dankook Univerity, Cheonan 330-714 and Research Center for Molecular Microbiology, Seoul Nationa University, Seoul 151-742, Korea, ^1Department of Food Engineering. College of Engineering, Dankook University, Cheonan 330-714 and Bioproducts Research Center of Yonsei University, Seoul 120-749, Korea - From the soil collected form provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete degraded during the microbial growth. Maximum cell growth (3.8×10^9 CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium that in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.
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