군산남고등학교

고기성,권중곤 현대건축사 2000 월간 CONCEPT Vol.- No.12

Glyco-engineering of Biotherapeutic Proteins in Plants

고기성,Mi-Hyun Ahn,Mira Song,Young-Kug Choo,Hyun Soon Kim,고기남,Hyouk Joung 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.4

Many therapeutic glycoproteins have been successfully generated in plants. Plants have advantages regarding practical and economic concerns, and safety of protein production over other existing systems. However, plants are not ideal expression systems for the production of biopharmaceutical proteins, due to the fact that they are incapable of the authentic human N-glycosylation process. The majority of therapeutic proteins are glycoproteins which harbor N-glycans, which are often essential for their stability, folding, and biological activity. Thus, several glyco-engineering strategies have emerged for the tailor-making of N-glycosylation in plants, including glycoprotein subcellular targeting, the inhibition of plant specific glycosyltranferases, or the addition of human specific glycosyltransferases. This article focuses on plant N-glycosylation structure, glycosylation variation in plant cell, plant expression system of glycoproteins, and impact of glycosylation on immunological function. Furthermore, plant glycoengineering techniques currently being developed to overcome the limitations of plant expression systems in the production of therapeutic glycoproteins will be discussed in this review.

P58IPK facilitates plant recovery from ER stress by enhancing protein synthesis

고기성,유재용,김경화,황보영,Vu Bich Ngoc,이영은,최하나,이유나,윤지희,박지예,정우식,홍종찬,정명선,정현석,정수경,박정미,이균오 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6

P58IPK has been implicated in eukaryotic ER stress responses and viral pathogenesis, however, its biological functions and molecular mechanism in plants are unclear. Prolonged ER stress produced by tunicamycin (TM) increased P58IPK mRNA and protein levels in Arabidopsis. Although the growth of 2×35S:P58IPK-myc plants was less severely inhibited than that of Col-0 plants, TM inhibited the growth of p58ipk-2 mutants more severely than that of Col-0 plants. Under prolonged ER stress conditions, the unfolded protein response (UPR)-related genes were expressed at a higher level in the p58ipk-2 mutants than in Col-0 plants. Protein synthesis inhibition by TM in 2×35S:P58IPK-myc plants was lower than in Col-0 plants under prolonged ER stress conditions, however, not signifcantly diferent in p58ipk-2 mutants. The GST-P58IPK protein exhibited both chaperone and RNA-binding activities in a dose-dependent manner. P58IPK has been shown to interact with ribosomes, allowing for enhanced protein production on the ER membrane. Following ER stress, 2×35S:P58IPK-myc plants recovered better than Col-0, but p58ipk-2 mutants recovered less than Col-0. These fndings reveal that P58IPK can promote protein translation in association with ribosomes and contribute to stress recovery in Arabidopsis when induced during the last phase of ER stress

기-고체 유동층에서 기포의 거동에 관한 연구

고기성 조선대학교 생산기술연구소 1982 生産技術硏究 Vol.1982 No.-

An electroresistivity probe was used to determine point properties of an air-fluidized bed of conducting coke particles. The effects of air volume and types of distributor on the bubble freqency distributions were observed at various radial positions(R) along the axial directon (H) of the column. Interruptions of electric current between the electrodes of a probe and a large wall electrode were analyzed with the aid of Brush Recorder (Model RD-2321-00) to yield the informations about bubble frequency as functions of various positions in bed. The results were as follows; 1. The bubble distributions in the column which attached a A-type distributor (described at Fig.3) were shown steady state above the positions of H=0.3. 2. The maximum bubble frequency when a B-type distributor was used in the column was observed at the position of R=0.8 and H=0.3. 3. Bubble frequencies at the various positions in the column showed a normal distribution below the flow velocity of 14.13㎤/sec. A theoretical model to predict the bubble frequency at a given position in the bubble column was proposed.

Cell cycle arrest mediated by WEE1 is involved in the unfolded protein response in plants

고기성,유재용,Nirmal Kumar Ramasamy,RIKNOHARMOKO,Bích Ngọc Thị Vũ,박지예,이균오 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5

Activation of the unfolded protein response (UPR) in mammalian cells leads to cell cycle arrest at the G1 phase (Thomas et al., J Biol Chem 288:7606–7617, 2013). However, how UPR signaling affects cell cycle arrest remains largely unknown in plants. Here, we examined UPR and endoreduplication in Col-0, wee1, and ER stress sensing-deficient ire1a&b plants during DNA replication and ER stress conditions. We found that WEE1, an essential negative regulator of the cell cycle, is involved in the maintenance of ER homeostasis during genotoxic stress and the ER stress hypersensitivity of ire1a&b is alleviated by loss-of-function mutation in WEE1. WEE1-mediated cell cycle arrest was required for IRE1–bZIP60 pathway activation during ER stress. In contrast, loss-of-function mutation in WEE1 caused increased expression of UPR-related genes during DNA replication stress. WEE1 and IRE1 were required for endoreduplication during DNA replication stress and ER stress, respectively. Taken together, these findings suggest that cell cycle regulation is associated with UPR activation in different manners during ER stress and DNA replication stress in Arabidopsis.

단일항체를 발현하는 형질전환 식물체의 In Vitro 화분발아에 대한 Boric Acid의 영향

고기성,Mi-Hyun Ahn,Kyung-Jin Lee 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.4

Pollen germination viability is an essential factor to produce seeds from pollination and fertilization, which are required to maintain plant generation. In this study, we tried to identify the effect of boric acid on pollen germination and tube growth in non-transgenic and transgenic plants expressing monoclonal antibodies (anti-colorectal cancer mAb CO17-1A, anti-breast cancer mAb BR55, and anti-rabies virus mAb57). The pollen of non-transgenic plant was treated with different concentration of boric acid (0, 5, 10, 15, 20, 40 μg/mL) in germination buffer to investigate its effect on in vitro pollen germination. At 20 μg/mL of boric acid, the pollen germination rate was the highest (49.5%) compared to other concentrations. In general, the germination rate significantly increased 3-10 folds in boric acid (20 μg/mL) treated group in non-transgenic and transgenic plants. Also, the pollen tube length increased in boric acid (20 μg/mL) treated groups. In the treated group, the pollen tube length increased until 3 h boric acid treatment and decreased after the 3 h, indicating that the 3 h is the most appropriate incubation time period. Western blot analysis showed that the mAb transgene expression was more stable in leaf than pollen in transgenic plants. This study suggested that 20 μg/mL of boric acid is ideal concentration to induce in vitro pollen germination of transgenic plants expressing therapeutic monoclonal antibodies, indicating stable pollination and fertilization in transgenic plants.

개발운용조직 통합관리(DevOps)기반 AI 무기체계 소프트웨어 개발에 대한 연구

고기성,박인수 국방기술품질원 2023 국방품질연구논집 Vol.5 No.1

Currently, research on unmanned combat robots, Manned-unmanned Teaming (MUM-T) systems, and big data utilization based on AI are being actively conducted in the field of weapon system software development, accelerating the unmannedization of the weapon system field. However, the traditional development method, known as the waterfall method theory, leads to the order of Requirement-development-test-deploy and has many limitations when applied to rapidly changing AI-based development. Therefore, in this study, the problems of the existing waterfall method and the methods for using DevOps in the AI-based weapon system software development process were studied.

항공전자를 위한 무연솔더(Sn-Ag-Cu)적용에 대한 연구

고기성(Gi Sung Ko) 한국항공우주학회 2017 한국항공우주학회 학술발표회 논문집 Vol.2017 No.11

군용항공기 탑재 소프트웨어의 안전필수기능경로분석(SCFTA) 모델 개발

고기성(Gisung Ko),고진환(Jinhwan Koh) 한국해군과학기술학회 2023 Journal of the KNST Vol.6 No.2

Software related to the safety critical function (SCF) used in military aircraft is designated as a safety support element (SSE), stricter development process should be applied. Therefore, it is very important to clearly identify software safety support elements in the early stages of aircraft development. However, the specific identification criteria are not clear and there are insufficient past cases to refer to, which are frequently subject to controversy at the development site. In this paper, the thread analysis model of the safety critical function of military aircraft software was studied through the analysis of the U.S. Air Forces Airworthiness Circular(AC-17-01). In addition, based on related research, the suitability of the method was verified by applying it to the actual military aircraft system development project, and a route analysis model was presented to ensure the stability and reliability of military aircraft software.

군용항공기 사이버보안에 대한 연구 및 발전방향

고기성(Gi-Sung Ko),장영현(Yong-Hyun Jang),박인수(In-Soo Park),고진환(Jin-Hwan Koh) 한국해군과학기술학회 2023 Journal of the KNST Vol.6 No.1

Currently, major developed countries are concentrating their capabilities to prepare for cyber attacks by enemy countries and organizations. In particular, aircraft need to be thoroughly prepared because cyber attacks can cause serious problems, including falls and casualties. Therefore, in this paper, we investigated the cases of the U.S. Navy, an advanced aircraft cybersecurity country, and presented research on cyber security systems that can be applied to Korean Navy and military aircraft and future development directions.