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급성림프구성백혈병의 면역학적 분류 및 림프아세포의 시험관내 분화유도에 관한 연구
서인석,조덕연,강원권,김삼용,이복희,이복수,백상기,김영진,이천배 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
The theory that various hematologic malignancies, such as acute and chronic leukemias and lymphomas, are characterized by monoclonal expansion of neoplastic cells arrested at different levels along the hemo-lymphopoietic differentiation pathway is widly accepted. It has also been supported by immunoglobulin gene rearrangement and phenotyping with specific antibodies to surface markers of lymphocytes concerning the lymphoid malignancies. In cope with these developments, the immunologic classification of acute lymphocytic leukemia had become more clarified. The author studied the immunologic classification of 15 patients with acute lymphocytic leukemia(ALL) with monoclonal antibodies against surface markers for lymphoid cells, and tried the in vitro induction of differentiation of progenitor-B cell ALL and T-cell ALL with TPA(12-0-tetradecanoyl phobol-13-acetate), a well known tumor promoter and known as a differentiation inducer also. Monoclonal antibodies for surface markers, used in this study, were Leu-9 for T-cell All and anti-Ia, anti-CALLA, anti-SIg(surface immunoglobulin), anti-BI, and anti-B4 for B-cell lineage ALL. Leu-9 positive cases were classified as T-cell ALL, Ia only positive cases were classified as progenitor-B cell ALL, Ia and CALLA positive cases were classified as common ALL, and Ia and SIg positive cases were classified as B-cell ALL. The results were as followings. 1. In this study, 5 cases were classified as common ALL, 3 each cases were B-cell and progenitor-B cell ALL, and 4 cases were T-cell ALL. 2. All 3 cases of B-cell ALL were L2 by FAB classification, and this findings was discordant with the conventional knowledge. 3. Leukocyte count in pheripheral blood of T-cell ALL patients at diagnosis was higher than that of non-T cell ALL(p<0.05). 4. All 5 cases of common ALL and 3 cases of B-cell ALL, 2 of 3 cases of progenitor-B cell ALL, and 1 of 4 cases of T-cell ALL achieved complete remission. So, T-cell ALL showed lower remission rate than that of common ALL and B-cell ALL. 5. One case of progenitor-B cell ALL, which is positive to only Ia, newly expressed B4, CALLA, BI and SIg after culture with TPA. This results suggest that TPA induce the differentiation of progenitor B-cells to more differentiated stage. 6. One case of T-cell ALL, which is positive to only Leu-9, newly expressed T3 after culture TPA and PHA. This results suggest that TPA and PHA induce the differentiation of early thymocyte to mature thymocyte.
急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구
고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2
To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.
이명극 ( Lee Myeong Geug ),강원권 ( Kang Won Gwon ),김민범 ( Kim Min Beom ),박철신 ( Park Cheol Sin ),이헌영 ( Lee Heon Yeong ),김삼용 ( Kim Sam Yong ),이복희 ( Lee Bog Hui ) 대한내과학회 1990 대한내과학회지 Vol.38 No.6
N/A To investigate the B-cell function in patients with chronic liver diseases, a peripheral blood T-cell subset, a delayed type skin reaction, serum levels of IgG, IgA, IgM and in vitro production of IgA, IgG were studied in patients with chronic liver diseases(10 cases of chronic hepatitis, 12 cases of liver cirrhosis). The results were as follows: 1) The percentage of peripheral blood suppressor T-cell(CD8 positive) decreased in patients with chronic hepatitis(p<0.05). 2) The results of delayed type skin reaction with CMI test showed no difference between normal subjects and patients with chronic liver diseases. 3) Serum IgG, IgA and IgM levels increased in patients with chronic hepatitis and in patients with liver cirrhosis(p<0.05). Serum IgA levels in patients with liver cirrhosis showed the most prominent increase(330% of control). 4) Unstimulated in vitro IgA production from peripheral blood mononuclear cell lymphocytes was higher in patients with liver cirrhosis(p<0.05). IgA production stimulated with pokeweed mitogen was not different from that of the normal control subjects. IgG production stimulated with pokeweed mitogen was lower in patients with liver cirrhosis(n=5)(p<0.05, Wilcoxon rank sum test). The results of the present study indicate that there is no major defect in cellular immunity in patients with chronic liver diseases and that the B cells in patients with chronic liver disease are polyclonally activated in vivo. In patients with liver cirrhosis, the IgA-producing B cells seem predominently activated in comparison with the IgG-or IgM-producing B-cells.
急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구
고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1
To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.