Alkaline protease 를 생산하는 미생물의 분리 , 동정 및 효소성질

강상모,신공식,고정연 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3

For the development of enzyme detergent capable of effectively washing at low temperature, a bacterium producing alkaline protease was isolated from soil samples, and properties of the enzyme were investigated. The selected strain was Gram negative, rod shape(0.6∼0.7×1.3∼2.6 ㎛ in size) and motile. It had the degradation activity of aesculin, gelatin and casein, and was catalase-positive. The cell wall components was meso-DAP, and G+C mole contents was 43.3%. From these results, the strain was identified as Acinetobacter sp. KN-27. The activity of alkaline protease by this strain peaked with 3,300 D.U/mL after 36 hours in the liquid culture at 40℃. The optimal pH and temperature of the enzyme were pH 9 and 60℃, respectively. Alkaline protease produced by Acinetobacter sp. KN-27 has shown two active bands on the electrophoresis of native gel.

Metabolism-mediated induction of zinc tolerance in Brassica rapa by Burkholderia cepacia CS2-1

강상모,Raheem Shahzad,Saqib Bilal,Abdul Latif Khan,유영현,이원희,유희라,이고은,이인중 한국미생물학회 2017 The journal of microbiology Vol.55 No.12

Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis. The strain actively produced indole-3-acetic acid (3.08 ± 0.21 μg/ml) and was also able to produce siderophore, solubilize minerals, and tolerate various concentrations of Zn+. The heavy metal tolerance of B. rapa plants was enhanced by CS2-1 inoculation, as indicated by growth attributes, Zn+ uptake, amino acid synthesis, antioxidant levels, and endogenous hormone (ABA and SA) synthesis. Without inoculation, the application of Zn+ negatively affected the growth and physiology of B. rapa plants. However, CS2-1 inoculation improved plant growth, lowered Zn+ uptake, altered both amino acid regulation and levels of flavonoids and phenolics, and significantly decreased levels of superoxide dismutase, endogenous abscisic acid, and salicylic acid. These findings indicate that B. cepacia CS2-1 is suitable for bioremediation against Zn+-induced oxidative stress.

Dielectrophoretic motions of multiple particles and their analogy with the magnetophoretic counterparts

강상모,Ranjith Maniyeri 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.11

To investigate the dielectrophoretic motions of multiple particles, we have performed the so-called direct numerical simulations on two-dimensional flows involving inertialess dielectric particles of two to five suspended in a viscous fluid under a uniform external electric field and then compared the results with those of the corresponding magnetophoretic counterparts. For the simulations, the electric field (or the force acting on each particle) is described by the numerical solution of the Maxwell equation (or Gauss's law), where the smoothed representation technique is employed to tackle the jump of electric conductivity across the particle-fluid interface. The flow field, on the other hand, is described by the solution of the continuity and momentum equations, where one-stage smoothed profile method is employed to satisfy the no-slip condition at the interface. In all the simulations, the particles are initially equi-spaced on a circle with an origin at the center while a uniform electric field is externally imposed. Results show that all particles move with repelling or attracting one another depending on the locally nonuniform electric field formed due to the presence of multiple particles. Consequently,they become clustered largely into two groups, then revolve in the clockwise or counterclockwise direction, and finally get aligned in a line with the field direction. One exception is their initial configuration which is symmetric with respect to the axis perpendicular to the electric-field direction, where all the particles move eternally far away from one another with keeping the symmetry. In addition, it is found that the two-dimensional relative motions of dielectric particles under a uniform external electric field are qualitatively in fairly exact agreement with those of paramagnetic particles suspended in a nonmagnetic fluid under a uniform external magnetic field.

일정속도로 회전하는 원형실린더를 지나는 주기적인 층류유동

강상모,최해천,이상산 한국항공우주학회 1998 한국항공우주학회 학술발표회 논문집 Vol.- No.-

Gibberellin-Producing Promicromonospora sp. SE188 Improves Solanum lycopersicum Plant Growth and Influences Endogenous Plant Hormones

강상모,Abdul Latif Khan,Muhammad Hamayun,Javid Hussain,주길재,유영현,김종국,이인중 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

Plant growth-promoting rhizobacteria (PGPR) producing gibberellins (GAs) can be beneficial to plant growth and development. In the present study, we isolated and screened a new strain of Promicromonospora sp., SE188, isolated from soil. Promicromonospora sp. SE188 secreted GAs into its growth medium and exhibited phosphate solubilization potential. The PGPR produced physiologically active (GA1 and GA4) and inactive (GA9, GA12, GA19, GA20, GA24, GA34, and GA53) GAs in various quantities detected by GC/MS-SIM. Solanum lycopersicum (tomato) plants inoculated with Promicromonospora sp. SE188 showed a significantly higher shoot length and biomass as compared to controls where PGPR-free nutrient broth (NB) and distilled water (DW) were applied to plants. The presence of Promicromonospora sp. SE188 significantly up-regulated the non C-13 hydroxylation GA biosynthesis pathway (GA12→GA24→GA9→GA4→GA34) in the tomato plants as compared to the NB and DW control plants. Abscisic acid, a plant stress hormone, was significantly down-regulated in the presence of Promicromonospora sp. SE188. Contrarily, salicylic acid was significantly higher in the tomato plant after Promicromonospora sp. SE188 inoculation as compared to the controls. Promicromonospora sp. SE188 showed promising stimulation of tomato plant growth. From the results it appears that Promicromonospora sp. SE188 has potential as a bio-fertilizer and should be more broadly tested in field trials for higher crop production in eco-friendly farming systems.

Growth and Gibberellins Level of Two Rice Cultivars as Influenced by Different Nitrogen Containing Compounds

강상모,Soo-Won Jang,Muhammad Hamayun,Eun-Young Sohn,Kwang-Il Choi,Dong-Hyun Shin 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.4

Seedlings of two rice cultivars i.e. cv. Daesanbyeo and cv. Dongjinbyeo were analyzed for growth and endogenous gibberellins(GAs) in response to nitrogen nutrition applied in the forms of KNO₃, (NH₄)₂SO₄ and NH₄NO₃. All the growth parameters showed anincrease in N applied treatments and their magnitudes of increase were different depending on different nitrogen fertilizer forms. Theendogenous GAs contents were increased with N application but differentially affected by various N-forms in both rice cultivars. Incv. Daesanbyeo, maximum amount of bioactive GA1 was recorded for (NH₄)₂SO₄, while maximum amount of bioactive GA₁ in cv. Dongjinbyeo was observed in NH4NO3 applied treatments. In both rice cultivars, KNO₃ applied rice plants contained least GA₁ contents. Also, GA19 was the most abundant GA found in rice seedlings whereas GA4 was absent at seedling stage. Our study indicatedthat different rice cultivars showed different responses for the same fertilizer depending upon the response potential of each cultivarand the pertinent physiological responses to changes of endogenous GAs in rice cultivars, which were comparatively lesser in magnitude.

Unsteady Electroosmotic Channel Flows with the Nonoverlapped and Overlapped Electric Double Layers

강상모,서용권 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.12

In micro- and nanoflows, the Boltzmann distribution is valid only when the electric double layers (EDL's) are not overlapped and the ionic distributions establish an equilibrium state. The present study has numerically investigated unsteady two-dimensional fully-developed electroosmotic flows between two parallel flat plates in the nonoverlapped and overlapped EDL cases, without any assumption of the Boltzmann distribution. For the study, two kinds of unsteady flows are considered: one is the impulsive application of a constant electric field and the other is the application of a sinusoidally oscillating electric field. For the numerical simulations, the ionic-species and electric-field equations as well as the continuity and momentum ones are solved. Numerical simulations are successful in accurately predicting unsteady electroosmotic flows and ionic distributions. Results show that the nonoverlapped and overlapped cases are totally different in their basic characteristics. This study would contribute to further understanding unsteady electroosmotic flows in micro- and nanofluidic devices.

살균소독수로 세척한 후 냉풍건조한 꽁치과메기의 품질특성

강상모,이원영 한국식품위생안전성학회 2015 한국식품위생안전성학회지 Vol.30 No.1

In this study, the microbial control effect after treatment of washing solutions(tap water, electrolyzed water, chlorine dioxide solution) and quality changes were investigated when Kwamegi is produced by means of the cold air drying. The initial moisture rate of the sample was 56.62% before drying. At the beginning of drying period, moisture rate sharply fell down but as the experiment progressed, moisture reducing rate was smoothly decreased. The color difference of Kwamegi before drying was 42.40, but it was reduced depending on the increment of drying temperature and time. TBA value of the initial sample was 0.219, rancidity were increased continuously when drying progressed. Total amino acid content was showed the highest value at 25℃ for 36h and the lowest at 40℃ for 12h. From the fatty acids analysis, major fatty acids were consisted of the 14:0, 16:0 and 18:1(18.15∼20.96%, 28.06∼32.51% and 17.06∼19.81%, respectively). The microbial control effect was biggest when Kwamegi was washed with chlorine dioxide 100ppm for 60s. The microbe of the Kwamegi, Pseudomonas sp. and Pseudomonas putida were identified.

국화류 추출물의 항산화 및 멜라닌 생성 억제 활성

강상모,강정란,이미경 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.4

The research was conducted to identify the antimicrobial effect, anti-oxidative effect and tyrosinase inhibitory effect of MeOH 80% extract and n-hexane, chloroform, ethyl acetate, n-butanol and water fractions from the extract of six kinds of compositae plants, which are naturally grown across the nation. In the antimicrobial effect, the extract and chloroform fraction of Arctium lappa and hexane/ ethyl acetate fractions of Taraxacum platycarpum exhibited significant inhibition. In case of antioxidant effect, the extract of Artemisia capillaries showed the highest effect and ethyl acetate/butanol fractions of all plants showed about 90%, which fractions were more polar than the fractions that showed antimicrobial effect. In case of tyrosinase activity, only the MeOH 80% of Arctium lappa among the extracts showed a potent inhibition, and butanol fraction of Chrysanthemum indicum, as well as ethyl acetate/water fractions of Artemisia capillaries showed 48, 38, and 37% respectively, which were higher than control group (arbutin). These active fractions in tyrosinase inhibition also were higher polarity than those that showed antimicrobial effect. In MeOH 80% extracts, only Arctium lappa was found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was no fraction to show effects commonly in the three assay system. The research was conducted to identify the antimicrobial effect, anti-oxidative effect and tyrosinase inhibitory effect of MeOH 80% extract and n-hexane, chloroform, ethyl acetate, n-butanol and water fractions from the extract of six kinds of compositae plants, which are naturally grown across the nation. In the antimicrobial effect, the extract and chloroform fraction of Arctium lappa and hexane/ ethyl acetate fractions of Taraxacum platycarpum exhibited significant inhibition. In case of antioxidant effect, the extract of Artemisia capillaries showed the highest effect and ethyl acetate/butanol fractions of all plants showed about 90%, which fractions were more polar than the fractions that showed antimicrobial effect. In case of tyrosinase activity, only the MeOH 80% of Arctium lappa among the extracts showed a potent inhibition, and butanol fraction of Chrysanthemum indicum, as well as ethyl acetate/water fractions of Artemisia capillaries showed 48, 38, and 37% respectively, which were higher than control group (arbutin). These active fractions in tyrosinase inhibition also were higher polarity than those that showed antimicrobial effect. In MeOH 80% extracts, only Arctium lappa was found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was no fraction to show effects commonly in the three assay system.

능동적 벽 운동을 수반하는 난류유동의 해석을 위한 근사 좌표변환

강상모,Gang, Sang-Mo 대한기계학회 2001 大韓機械學會論文集B Vol.25 No.11

In the present paper, approximate coordinate transformations for simulation of turbulent flows with active wall motions, leading to a significant reduction in the computational cost while maintaining the numerical accuracy, are presented: the Navier-Stokes equations are coordinate-transformed with an approximation of Taylor-series truncation and neglect of some less-significant terms. The performance of the proposed transformations is evaluated in simulation of the channel flow at Re$\sub$$\tau$/=140 with wall deformations of │η$\sub$m/$\^$+/ 5. The approximate transformations provide flow structures as wall as turbulence statistics in good agreement with those from a complete coordinate transformation [Phys. Fluids 12, 3301 (2000)] and allow 25-30% savings in the CPU time as compared to the complete one.