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김무성,이동철,이성구,강병영,선보경,안수선,심영철,강학희 대한화장품학회 2000 대한화장품학회지 Vol.26 No.1
Development of stabilized enzyme was attempted for cosmetic applications. Papain, a proteolytic enzyme, was stabilized through conjugation with a soluble carbohydrate biopolymer, SC-glucan™. With a novel structure of the conjugation site, stability of the enzyme was significantly enhanced such that more than 90 % of the initial activity retained after a month storage at 45℃, while no activity were detected in native enzyme or enzyme simply mixed with SC-glucan™ after the storage. Conjugation with SC-glucan™ not only extended the half-life of the enzyme on storage at higher temperature, but was also found to protect enzymes against some components contained in cosmetic products for skin care. Cosmetic lotion containing 1 % papain conjugate was more effective and less irritative in exfoliating stratum corneum of human skin than the lotion containing 5% lactic acid, one of the current popular exfoliating agents.