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김용욱,김태현,양민아,원예슬,이지혜 KEMA학회 2017 근골격계과학기술학회 Vol.1 No.1
Background Previous studies reported that one-leg squat has various advantages in functional activities, no study has studied the effect of muscle activity on ankle stability. Purpose The purpose of this study was to investigate the differential effects of squat type on the activity of the tibialis anterior, peroneus longus and tibialis posterior muscle for ankle stability. Study design Cross-sectional study. Methods The participants were 30 healthy adults. During each squat execution, tibialis anterior, peroneus longus and tibialis posterior muscle activities were assessed using surface electromyography under control of each squat posture. Results The muscle activity of the tibialis anterior and peroneus longus applying Bulgarian split squat was significantly higher than lunge squat. However, the activity of the tibialis posterior showed no significance between squat conditions. Conclusions This study demonstrated that Bulgarian squat was more effective at eliciting ankle dorsiflexor and evertor that are known as important role for ankle stability. Background Previous studies reported that one-leg squat has various advantages in functional activities, no study has studied the effect of muscle activity on ankle stability. Purpose The purpose of this study was to investigate the differential effects of squat type on the activity of the tibialis anterior, peroneus longus and tibialis posterior muscle for ankle stability. Study design Cross-sectional study. Methods The participants were 30 healthy adults. During each squat execution, tibialis anterior, peroneus longus and tibialis posterior muscle activities were assessed using surface electromyography under control of each squat posture. Results The muscle activity of the tibialis anterior and peroneus longus applying Bulgarian split squat was significantly higher than lunge squat. However, the activity of the tibialis posterior showed no significance between squat conditions. Conclusions This study demonstrated that Bulgarian squat was more effective at eliciting ankle dorsiflexor and evertor that are known as important role for ankle stability.
양다래X다래 클론 118의 엽조직 캘러스를 이용한 세포 현탁배양으로부터 식물체 유도
김용욱,문흥규,Kim Yong-Wook,Moon Heung-Kyu 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4
Actinidia deliciosa ${\times}$ A. arguta clone 118의 엽조직을 0.5 mg/L 2,4-D, 0.1 mg/L NAA 및 0.05 mg/L BA가 첨가된 MS 배지에서 8주간, 광조건하에서 배양하여 캘러스를 유기시켰다. 유도된 캘러스는 현탁배양을 위해 0.5 mg/L 2,4-D, 0.1 mg/L NAA 및 0.05 mg/L BA가 첨가된 액체 MS배지로 접종시켰다. 현탁배양세포의 대수생장기 혹은 생장 정체기 시기는 세포배양 5-11일, 12일경에 각각 관찰되었다. 현탁세포로부터 유도된 캘러스의 생중량은 생장조절물질 조합에 따라서 차이를 보이지 않았다. 가장 높은 신초유도율 (88.3%)은 2.0 mg/L zeatin의 첨가구였으며 TDZ과 BA 혹은 zeatin이 혼합처리된 처리구에서 또한 신초유도에 효과가 있는 것으로 나타났다. 유도된 신초를 MS +0.2 mg/L zeatin으로 옮겨 더욱 신초생장을 유도하였으며 발근유도를 위해 1.0 gm/L IBA가 첨가된 St배지로 옮겼다. Actinidia deliciosa ${\times}$ A. arguta clone 118의 세포배양으로부터 식물체 재분화까지 가능하였다. Calli were induced by culturing the leaf segment of Actinidia deliciosa ${\times}$ A. arguta clone 118 on MS medium supplemented with 0.5 mg/L 2,4-D, 0.1 mg/L NAA and 0.05 mg/L BA for 8 weeks in light condition. The induced calli were inoculated in liquid MS medium containing 0.5 mg/L 2,4-D, 0.1 mg/L NAA, 0.05 mg/L BA and 3% sucrose to establish cell suspension culture. The cells at the exponential stage and the stationary stage could be observed between 5-11 days and after that 12 days in culture, respectively. The fresh weight of callus induced from the suspended cells did not vary much among the media containing eight different combinations of plant growth regulators tested. The highest frequency of shoot induction (88.3%) was observed in MS medium containing 2.0 mg/L zeatin. Either BA or zeatin mixed with thidiazuron (TDZ) seemed to be effective in shoot induction. The induced shoots were transferred to MS medium containing 0.2 mg/L zeatin for further shoot growth. And then the shoots were transferred to Standardi (ST) medium containing 1.0 mg/L indolebutyric acid (IBA) for rooting. Plantlets could be obtained through cell suspension culture of Actinidia deliciosa ${\times}$ A. arguta clone 118.