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      • 간호의 책임 : 빈곤 속의 아동 Childhood in poverty

        강희경 제주한라대학 1997 論文集 Vol.21 No.-

        Definition of poverty as a modifiable health risk is crucial to nursing, as nursing is committed to protecting and improving the health of disadvantaged populations. The theme of this article is "the health of childhood in poverty as a disadvantaged population". Since most of society's policies and programs are ostensibly based on research findings, the clarity, or its lack, in nursing's choice is critical in health care for poor children. For the theme of this article, work of Jurgen Habermas, the most prolific contemporary critical theorist, was briefly reviewed. In Harbermas' eyes, Poverty is a process that have dehumanized children. And, this process is a need to move beyond consideration of poverty as a variable to be controlled and to examine the relationship between poverty and health more carefully. Suggestions to enhance further investigation of this relationship include · Identification and clarification of the conceptual frameworks guiding inquiry into the relationship between health and poverty in childhood · Broadening the definitions of poverty providing operationalization · Developing the ideas about programs that can be integrated and translated into policy for children in poverty Conclusively, nursing is described as extending beyond the health care system and into the broader environment to include poverty affecting health. Poverty, therefore, is a modifiable health risk that can be considered within the scope of nursing practice.

      • 학령기 아동의 사회적 지지와 자기평가

        강희경 한국부모교육학회 2011 부모교육연구 Vol.8 No.1

        This study investigated the relationship between social supports and the self-evaluation of school-aged children. The Social Supports Scale is composed of three factors : social supports from family, friends and teachers. The subjects were 397 school-aged children. The main results were as follows. First, the level of social supports was generally high, with the highest level being from family. Second, the level of self-evaluation was also generally high. Finally, all supports from family, friends and teacher had positive influences on self-evaluation. The most influential factor was social support from friends. The implications of these results were discussed.

      • 간호진단과 컴퓨터 : 간호교육의 중심 테마 The Central Theme in Nursing Education

        강희경 제주한라대학 1993 論文集 Vol.17 No.-

        In this article, nursing diagnosis and computer assumed as the central theme in nursing education were reviewed. These are buildings as skills of survivals for nursing within other disciplines and guides for the domain of nursing within health care system. First, the view is taken that nursing diagnoses provide a way of conceptualizing the domain of nursing and a focus for theory development. Nursing diagnosis appear to be catching the attention of many in the nursing arenas of practice, administration, research, and education. Second, history and future of the computer in nursing education were reviewed. Nursing educators are beginning to realize that computer literacy has become a survival skill for the profession. They suggest the openness and awareness for future technologic possibilities. Also, the evolution of formal coursework in computer literacy has moved from learning "about the computer" to learning "with the computer". Today the use of the computer is expanding geometrically as microcomputers become common. Graduate students and faculty use them for literature searchs and data analysis. Undergraduates are routinely using computer-assisted instruction. Coursework in computer technology is fast becoming a given for nursing students and computer competency a requisite for faculty. Therefore, computer literacy becomes a necessity "for Heaven and the future's sake". Finally, the computer strategies for teaching nursing diagnosis were introduced. Computer techniques such as EXTEND (EXperT System for Education in Nursing Diagnosis) and ART (Adaptive Resonance Theory)-2 Neural Network et al. can provide new avenues for students and educators in nursing.

      • 飮食物 堆肥 施用 따른 벼의 生育 및 土壤 化學的 特性의 變化

        김성민,신동일,윤영상,안현진,강희경 公州大學校 産業開發硏究所 1999 産業開發硏究 Vol.7 No.-

        This study was carried out to investigate of rice plant growth reaction and sodium action in compost by using food -waste compost in pot. From this test we on get basic data using food-waste compost in real paddy soil. Wilting by application rate food-wast compost 4ton/10a, next NaCl 90kg/10a, food-waste compost 2ton/10a. Heading stage is not different from application NaCl 60kg/10a to control pot of applicates showed from N-P-K, but applicating NaCl 90kg/10a was decreased 17%. Voltile organic acid control content was increased by applicating food-waste compost 37 days after planting from the first stage of rice growth, and applicating food-waste compost was showed more increased volitle organic acid content. Bubble was showed from food-easte compost 4t/10a application long term, is considered poison by H₂S gas at reduction environment. It is very important fact volatile organic acid was occured at food-waste compost application field 37 days after planting. So it was needed to conder4ing production process, application duration, application rates and of food-waste compost concentration, poison material and volatile organic acid.

      • 급성 골수성 백혈병 세포의 성장에 대한 비타민 C의 효과

        강희경 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

        The purpose of the present study is to examine the effect of vitamin C on the growth of leukemia cells. The effect was observed using KG-1 cell line which was derived from bone marrow of acute myelogenous leukemia patient. When KG-1 cells were grown in liquid media in the presence of vitamin C at the concentrations of 10-6 M to 10-3 M, the growth of these cells was stimulated up to 10-4 M and was inhibited at higher concentrations(≥ 5 X 10-4 M). The difference of growth according to the concentration of vitamin C was also observed in semisolid culture. The induction of differentiation on KG-1 cells by vitamin C was assessed by expression of cell surface antigens, CD 66b or CD 14 antigens. At low concentrations (10-6 to 10-4 M), vitamin C had little capacity to induce differentiation of KG-1 cells. In contrast, it was found that at higher concentrations(≥ 5 X 10-4 M) vitamin C-treated KG-1 cells showed activity to express CD 66b or CD 14 antigens. The results indicate that the degree of growth inhibition of KG-1 is in parallel with that of differentiation into granulocytes and monocytes. Therefore, inhibitory effect of vitamin C on the growth of KG-1 seems to rise from the induction of differentiation.

      • 被覆材料가 구약감자의 生育에 미치는 影響

        金成敏,蔡濟天,姜熙慶 公州大學校 資源科學硏究所 1995 資源科學硏究論文集 Vol.3 No.-

        구약감자栽培에 적합한 被覆材料의 選拔과 球莖重에 따른 被覆材料別 生育特性을 究明하고자 3種類의 被覆材料로 試驗한 結果는 다음과 같다. 1. 出芽率은 볏짚피복구에서 가장 놓았으며 球莖重이 큰 種薯일수록 出芽率이 빨랐다. 2. 지상부 枯死率은 무피복구가 가장 낮았고 種薯의 球莖重이 작을수록 지상부 枯死率이 낮았다. 3. 球莖의 增大와 子球의 發達은 볏짚피복구와 흑색차광막 피복구 모두에서 良好하였고 고추멀칭용비닐 피복구의 子球發生量은 매우 적었다. This study was carried out to know the effects of mulching with rice straw, black polyethylene net and black vinyl in Amorphophallus konjac K. cultivation. The emergence rate of rice straw mulching plot was higher than other mulching material plots, and emergence period was reduced in large corm size plot. Withering rate of arial part was the lowest in non-mulching plot and that was also low in small size of corms. Both enlargement of corm weight and development of cormlet were excellent at rice straw mulching plot and black polyethylene net plot, but development of cormlets at black vinyl plot was very poor.

      • KCI등재

        Cloning of Levansucrase from Leuconostoc mesenteroides and Its Expression in Pichia pastoris

        강희경,김도만,Sang-Il Yun,Tae Yoon Lim,Yong-mei Xia 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.1

        A gene (m1ft) encoding levansucrase from Leuconostoc mesenteroides has been previously cloned in Escherichia coli. Presently, m1ft was cloned and secretively expressed in Pichia pastoris. Methanol induction of recombinant M1FT resulted in the production of active levansucrase (PM1FT). PM1FT-5 was expressed as an active form, but the protein accumulated mainly inside the cells, representing about 5% of total cell proteins. M1FT was secreted into the culture supernatant with a maximum yield of 14,400 U/L using fed-batch fermentations. P. pastoris-derived M1FT displayed catalytic activities comparable to those of the E. coli-derived M1FT. PM1FT was glycosylated at its 2 potential N-glycosylation sites.

      • SCOPUSKCI등재

        토양으로부터 Cryptococcus sp. CS-2의 분리 및 균주가 분비하는 Polygalacturonase의 특성에 관한 연구

        강희경,문명님,임채영,양영기 한국미생물학회 1999 미생물학회지 Vol.35 No.2

        제주도 밀감 과수원의 토양으로부터 polygalacturonase 고생산 효모를 분리하였다. 분리된 strain CS-2 의 생리화학적인 실험을 수행한 결과 분리균주는 Diazonium bule B color test 와 urease test에서 양성반응, acetic acid, citrate acid 생성 시험, gelatin 분해시험, fat 분해시험에서는 음성반응을 나타났고, 탄소원의 조사에서 galactose를 탄소원으로 이용할 수 없었고, 질소원은 모두 이용할 수 없었다. 또한 $30^{\circ}C$ 에서는 성장하는 반면, $35^{\circ}C$ 이상에서는 성장을 하지 못하였고 50% 이상의 glucose 농도, 0.01% cyclohexamide 그리고 1% acetic acid에서 성장을 하지 못하였다. 현미경을 이용한 형태학적 관찰 결과, 크기는 $1.3{\times}2.9$$\mu$m인 타원형으로 관찰되었다, ,multiple budding을 하며 ascospore는 존재하는 반면 pseudomycelium과 true mycelium 은 존재하지 않았다. 이와 같은 결과를 종합하여 Cryptococcus 속의 특징과 유사함을 알 수 있었다. 분리된 Cryptococcus sp. CS-2 의 polygalacturonase 는 유도적으로 합성되어 catabolic repression 에 의해 조절됨을 알 수 있었으며, 3일 배양시 가장 높은 활성도를 나타내었으며, 고유 활성도는 2.50~2.55 units/mg 으로 나타났다. 이러한 polygalacturonase를 SDS-PAGE, activity staining 그리고 단일 탄소원에 따른 단백질 양상의 변화를 비교하여 분자량 측정한 결과 약 46KDa으로 나타났다. A ploygalacturonase-produchg yeast was isolated from Cheju soil by selective eivichment media. One strain which has the highesl activity of polygalacturonase was selected. The characle~ishcs of the strain CS-2 were as follows: CS-2 utilized xylose. sucrose, maltose, u.ehalose, cellobiose. melibiose, lactose, raffinose, inosiiol, dulicilol, and dextrose, but did not utilized galactose, nitrate. nit~te, and lysine. Growth of CS-2 was inhibited by cyclohexamide, 1% acetic acid, and high concenaation (over 50%) of glucose. It grew at $30^{\circ}C$ but did 'IIOL $35^{\circ}C$. The cell size ofthe strain CS-2 was 2.9 p ~ n in length and 1.3 $\mu$ in diameter. Vegetable reproductmn was multiple budding and ascospre was present I to 4. Pseudomycelia or true myceliua formation were not observed In any of the cullureq. These results suggest that strain CS-2 is most likely a strain related Cryptococcus spp. (Cryptococcu spp. CS-2). When polygalacturonase or ihe yeast was induced by addition of polygalactoronic acid, polygalacturonase activity was detected in culture supernatent. There was a peak of specific activity a1 he mid-stationary phase(3 days culture) of growth. Polygalacturonase specific activity of Crylmcoccus sp. CS-2 was 2.96 unitsling. The molecular weighl ol'polygalacturonase was showed to be 46 KDa by both SDS-PAGE and activity stailling.

      • KCI등재
      • IRRI에서 육성한 벼 흰잎마름병 저항성 Near-isogenic lines의 몇가지 작물학적 특성

        홍성찬,윤영상,강희경 公州大學校 資源科學硏究所 1999 資源科學硏究論文集 Vol.7 No.-

        This study was carried out to evaluated the reaction of near-isogenic lines bred in IRRI with single genes for bacterial blight resistance to three Korean races of Xnathomonas oryzae pv. oryzae, rice blast fungus and some agronomic characters. Agrinimic characters of near-isogenic lines bred in IRRI largely similar to recurrent parents, but some lines showed to some degree different compared to recurrent parents. Near-isogenic lines derived from crosses using IR 24 and Milyang 23 as recurrent parents showed resistant to leaf blast and near-isogenic lines with Xa-3.Xa-4,Xa-5and Xa-7 resistant genes showed against bacterial blight of rice to three Korean races(K1,K2,K3). These near-isogenic lines were judged good sources for rice breeding

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