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Seungmin Jeong,Chaeyun Lim,Sunhak Bae,Youngju Nam,Eunmi Kim,Myeonggi Kim,Saerom Kim,Yeojin Kim 질병관리본부 2023 Osong Public Health and Research Persptectives Vol.14 No.3
Objectives: This study aimed to examine the correlations between the regional characteristics of counties in Gangwon Province, Republic of Korea and the ratio of intracounty to extracounty sources of coronavirus disease 2019 (COVID-19) infection.Methods: The region of the infectious contact was analysed for each COVID-19 case reported in Gangwon Province between February 22, 2020 and February 7, 2022. The population, population density, area, the proportion of urban residents, the proportion of older adults (>65 years), financial independence, and the number of adjacent counties were assessed for each of the 18 counties in Gangwon Province. Correlation coefficients between regional characteristics and the ratio of intracounty to extracounty infections were calculated.Results: In total, 19,645 cases were included in this study. The population, population density, proportion of older adults, and proportion of urban residents were significantly correlated with the ratio of intracounty to extracounty infections. A stratified analysis with an age cut-point of 65 years showed that the proportion of older adults had a significant negative correlation with the ratio of intracounty to extracounty infections. In other words, the proportions of extracounty infections were higher in countries with higher proportions of older adults.Conclusion: Regions with ageing populations should carefully observe trends in infectious disease outbreaks in other regions to prevent possible transmission.
Kim, Ju-Myung,Kim, Chanhoon,Yoo, Seungmin,Kim, Jeong-Hoon,Kim, Jung-Hwan,Lim, Jun-Muk,Park, Soojin,Lee, Sang-Young The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.20
<▼1><P>We demonstrated an agarose-biofunctionalized, dual-electrospun heteronanofiber mat as a new class of chemically active (specifically, metal-ion chelating) separator membranes for high-performance Li-ion batteries.</P></▼1><▼2><P>A facile and efficient way to impart compelling chemical functionality is the utilization of bio-related materials that are easily accessible from natural products. Here, inspired by anomalous physicochemical features and natural abundance of agarose, we demonstrate a new class of agarose-biofunctionalized, dual-electrospun heteronanofiber mats as a chemically active separator membrane for high-performance lithium-ion batteries. The agarose-enabled metal ion chelation effect of the separator membrane, in combination with its highly porous structure and superior electrolyte wettability, provides unprecedented improvement in cell performance far beyond those accessible with conventional battery separator membranes.</P></▼2>
Kim, Ryunhee,Kim, Jihye,Chung, Changuk,Ha, Seungmin,Lee, Seungjoon,Lee, Eunee,Yoo, Ye-Eun,Kim, Woohyun,Shin, Wangyong,Kim, Eunjoon Society for Neuroscience 2018 The Journal of neuroscience Vol.38 No.17
<P>Shank2 is an excitatory postsynaptic scaffolding protein implicated in synaptic regulation and psychiatric disorders including autism spectrum disorders. Conventional Shank2-mutant (Shank2(-/-)) mice display several autistic-like behaviors, including social deficits, repetitive behaviors, hyperactivity, and anxiety-like behaviors. However, cell-type-specific contributions to these behaviors have remained largely unclear. Here, we deleted Shank2 in specific cell types and found that male mice lacking Shank2 in excitatory neurons (CaMKII-Cre;Shank2(fl/fl)) show social interaction deficits and mild social communication deficits, hyperactivity, and anxiety-like behaviors. In particular, male mice lacking Shank2 in GABAergic inhibitory neurons (Viaat-Cre;Shank2(fl/fl)) display social communication deficits, repetitive self-grooming, and mild hyperactivity. These behavioral changes were associated with distinct changes in hippocampal and striatal synaptic transmission in the two mouse lines. These results indicate that cell-type-specific deletions of Shank2 in mice lead to differential synaptic and behavioral abnormalities.</P>
Direct Electrodeposition of Cu on Ru-Al<sub>2</sub>O<sub>3</sub> Layer
Kim, Myung Jun,Kim, Hoe Chul,Kim, Soo-Hyun,Yeo, Seungmin,Kwon, Oh Joong,Kim, Jae Jeong The Electrochemical Society 2013 Journal of the Electrochemical Society Vol.160 No.12
<P>This study introduces the direct electrodeposition of Cu on Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layers, which is a promising material to replace Cu seed, and Ta diffusion barrier layers at once. Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layers are deposited by atomic layer deposition (ALD), and their compositions are manipulated by varying the cycle numbers of Ru and Al<SUB>2</SUB>O<SUB>3</SUB> ALD. The addition of Al<SUB>2</SUB>O<SUB>3</SUB> induces the development of nanocrystalline Ru, instead of columnar structure, enhancing the characteristics as a diffusion barrier without losing a role of the seed layer for Cu electrodeposition. The native oxide of Ru is electrochemically eliminated by the coulometric reduction method (CRM) prior to Cu electrodeposition. The influences of both applied potential and the composition of Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer on Cu film properties are clarified. Furthermore, the ability of Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer to inhibit Cu diffusion is assessed, and it is confirmed that Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer perfectly inhibits Cu diffusion during the annealing carried out at the temperature of 550°C for 30 min with the layer thickness of 7.5 nm.</P>
Kim, Bong Jik,Ueyama, Takehiko,Miyoshi, Takushi,Lee, Seungmin,Han, Jin Hee,Park, Hye-Rim,Kim, Ah Reum,Oh, Jayoung,Kim, Min Young,Kang, Yong Seok,Oh, Doo Yi,Yun, Jiwon,Hwang, Sang Mee,Kim, Nayoung K D BMJ Publishing Group Ltd 2019 Journal of medical genetics Vol.56 No.12
<P><B>Background</B></P><P>Diaphanous-related formin 1 (DIA1), which assembles the unbranched actin microfilament and microtubule cytoskeleton, is encoded by <I>DIAPH1</I>. Constitutive activation by the disruption of autoinhibitory interactions between the N-terminal diaphanous inhibitory domain (DID) and C-terminal diaphanous autoregulatory domain (DAD) dysregulates DIA1, resulting in both hearing loss and blood cell abnormalities.</P><P><B>Methods and results</B></P><P>Here, we report the first constitutively active mutant in the DID (p.A265S) of humans with only hearing loss and not blood cell abnormality through whole exome sequencing. The previously reported DAD mutants and our DID mutant (p.A265S) shared the finding of diminished autoinhibitory interaction, abnormally upregulated actin polymerisation activity and increased localisations at the plasma membrane. However, the obvious defect in the DIA1-driven assembly of cytoskeleton ‘during cell division’ was only from the DAD mutants, not from p.A265S, which did not show any blood cell abnormality. We also evaluated the five DID mutants in the hydrophobic pocket since four of these five additional mutants were predicted to critically disrupt interaction between the DID and DAD. These additional pathogenic DID mutants revealed varying degrees of defect in the DIA1-driven cytoskeleton assembly, including nearly normal phenotype during cell division as well as obvious impaired autoinhibition, again coinciding with our key observation in DIA1 mutant (p.A265S) in the DID.</P><P><B>Conclusion</B></P><P>Here, we report the first mutant in the DID of humans with only hearing loss. The differential cell biological phenotypes of DIA1 during cell division appear to be potential determinants of the clinical severity of <I>DIAPH1-</I>related cytoskeletopathy in humans.</P>
Effect of temperature on single-and mixed-strain fermentation of ruminant feeds
( Seungmin Woo ),( Sooah Kim ),( Suji Ye ),( Soo Rin Kim ),( Jeongman Seol ),( Uyeh Daniel Dooyum ),( Junhee Kim ),( Dong Hyuck Hong ),( Jong Nam Kim ),( Yushin Ha ) 한국축산학회(구 한국동물자원과학회) 2020 한국축산학회지 Vol.62 No.2
Use of raw feedstuffs for livestock is limited by low digestibility. Recently, fermentation of feedstuffs has been highlighted as a new way to improve nutrient absorption through the production of organic acids using inoculated microorganisms, which can also play a probiotic role. However, standard procedures for feedstuff fermentation have not been clearly defined because the process is influenced by climatic variation, and an analytical standard for fermented feedstuffs is lacking. This study aimed to evaluate the microbiological and biochemical changes of feedstuffs during fermentation at temperatures corresponding to different seasons (10℃, 20℃, 30℃, and 40℃). We also investigated the effects of yeast, lactic acid bacteria (LAB), and Bacillus spp. on fermentation and determined the results of their interactions during fermentation. The viable cells were observed within 8 days in single-strain fermentation. However, when feedstuffs were inoculated with a culture of mixed strains, LAB were predominant at low temperatures (10℃ and 20℃), while Bacillus spp. was predominant at high temperatures (30℃ and 40℃). A significant drop in pH from 6.5 to 4.3 was observed when LAB was the dominant strain in the culture, which correlated with the concentrations of lactic acid. Slight ethanol production was detected above 20℃ regardless of the incubation temperature, suggesting active metabolism of yeast, despite this organism making up a marginal portion of the microbes in the mixed culture. These results suggested that fermentation temperature significantly affects microbiological profiles and biochemical parameters, such as pH and the lactic acid concentration, of fermented feedstuffs. Our data provide valuable information for the determination of industrial standards for fermented feedstuffs.
Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test
Kim Kang Hyeon,유용경,Lee Na Eun,Lee Junwoo,Kim Cheonjung,Lee Seungmin,Kim Jinhwan,Park Seong Jun,Lee Dongtak,이상원,Kim Hyungseok,허돈,Yoon Dae Sung,Lee Jeong Hoon 한국바이오칩학회 2023 BioChip Journal Vol.17 No.3
Lateral fl ow assay (LFA) is a popular diagnostic system used in point-of-care testing (POCT) due to its low cost and portability. However, LFA has limited sensitivity and detection limits, making it challenging to detect low virus titers. Preconcentration through nano-electrokinetic (NEK) techniques have been proposed as a promising solution to improve the sensitivity of LFA. Nevertheless, the acidic conditions used in NEK operations may reduce the specifi city and sensitivity of LFA immunoassays. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK–LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration. Biomarkers and AuNPs are electrokinetically preconcentrated in the PcNEK–LFA platform to increase the concentration of the test line and Ag–Ab binding events, resulting in enhanced performance. The pH-controlled PcNEK–LFA platform was evaluated using salivary human chorionic gonadotropin beta (β-hCG) and COVID-19 Ag samples, achieving a preconcentrating factor of approximately 10 and a sensitivity enhancement of 55.42%, and a preconcentrating factor greater than 10, respectively. The pH-controlled PcNEK–LFA platform provides an eff ective solution to overcome the limitations of LFA for POCT. In addition, it improves its sensitivity and detection limit, signifi cantly enhancing the accuracy and reliability of POCT, particularly for COVID-19 screening tests. As a result, this platform may play a pivotal role in addressing current and future healthcare challenges, facilitating rapid diagnosis and treatment of infectious diseases.
Antioxidant and Neuroprotective effects of Gochujang Supplemented with Functional Ingredients
Sunghee Kim,Yunjeong Gwon,Jungeun Kim,Seungmin Park,Siyul Byeon,Janice Nullan Averilla,Ji Sun Lim,Chan Ho Jang,Jisun Oh,Myeong Hui Choi,Jun Young Kim,Jong-Sang Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10