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범가자미, Verasper variegatus의 생식소 발달단계에 따른 혈중 난황단백전구체 (vitellogenin)와 성 스테로이드 호르몬 변화
한창희,會田勝美,김윤,백혜자,小林牧人 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
Annual plasma levels of vitellogenin and sex steroids were investigated in relation to the gonadal development for understanding the endocrine control of reproduction in spotted flounder, Verasper variegatus. The plasma vitellogenin level was highest, 6.36㎎/㎖, in November when vitellogenesis was most active. The level, thereafter, decreased to 3.81㎎/㎖ in December with the initiation of spawning. On the other hand, estradiol-17 β was highest, 2.7ng/㎖, in December, and rapidly decreased in January when spawning occurred. The decreased level of estradiol-17 β, around 0.2ng/㎖, remained unchanged until May. The profiles of plasma testosterone were similar to those of estradiol-17 β in the fish. The Plasma 17 α-hydroxyprogesterone level was relatively low throughout the spawning period, but increased slightly with the initiation of ovarian development. In males, the plasma testosterone and 11- ketotestosterone were highest in December when spermiation actively proceeded, but rapidly decreased during the spawning period (January).
Ko, Hyeyeon,Park, WooDong,Kim, Dae-Jung,Kobayashi, Makito,Sohn, Young Chang Journal of Endocrinology (Ltd. by Guarantee) 2007 Journal of molecular endocrinology Vol.38 No.1
<P>Gonadotropins (GTHs), FSH and LH, play central roles in vertebrate reproduction. Here, we report the production of biologically-active recombinant FSH (r-mtFSH) and LH (r-mtLH) of an endangered salmon species, Manchurian trout (Brachymystax lenok), by baculovirus in silkworm (Bombyx mori) larvae. The biological activities of the recombinant hormones were analyzed using COS-7 cell line transiently expressing either amago salmon FSH or LH receptor. The steroidogenic potency of the r-mtFSH and r-mtLH was examined by a culture system using rainbow trout follicles in vitro. In vivo, bioactivity was assessed by measuring ovarian weight, oocyte diameter, and plasma steroid hormone levels in female rainbow trout. Moreover, inducing potency of milt production were examined in vivo using goldfish. Our results demonstrated that the r-mtFSH and r-mtLH were successfully produced in the baculovirus-silkworm system and recognized by their cognate receptors specifically in vitro. The production of estradiol-17beta (E2) and testosterone (T) was stimulated by the r-mtFSH and r-mtLH respectively, from the full-grown follicles of rainbow trout, whereas both E2 and T were increased by relatively higher doses of the recombinant hormones from the follicles of the maturing stage. In in vivo assay, injection of the r-mtFSH but not r-mtLH increased ovarian weight, oocyte diameter, and plasma E2 levels in immature rainbow trout. Injection of both r-mtFSH and r-mtLH induced milt production in male goldfish. In conclusion, the present study strongly suggests that the r-mtFSH and r-mtLH have distinct biological properties, such as a specific responsiveness for the cognate receptor, steroidogenic, and vitellogenic activities for ovarian follicles in salmonids. These recombinant FSH and LH may be applied for future studies on the gonadal development and maturation in fishes as well as the endangered salmon species.</P>
Yokoyama, Yoshihito,Shigeto, Tatsuhiko,Miura, Rie,Kobayashi, Asami,Mizunuma, Makito,Yamauchi, Aisa,Futagami, Masayuki,Mizunuma, Hideki Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.2
Background: The current study examined the effectiveness of concurrent therapy using photodynamic therapy (PDT) and clofibric acid (CA) to treat peritoneal carcinomatosis resulting from ovarian cancer. Materials and Methods: Nude rats were used to create a model of peritoneal carcinomatosis resulting from ovarian cancer and the effectiveness of PDT with 5-aminolevulinic acid methyl ester hydrochloride (methyl-ALA-PDT) was determined. The survival time of rats receiving that therapy was compared to the survival time of a control group. Rats with peritoneal carcinomatosis resulting from ovarian cancer were divided into 3 groups: a group that received debulking surgery (DS) alone, a group that received DS+methyl-ALA-PDT, and a group that received DS+methyl-ALA-PDT+CA. The survival time of the 3 groups was compared. Protoporphyrin, a metabolite of methyl-ALA, produces a photochemical action when activated by light. The level of protoporphyrin (the concentration) that reached organs in the abdomen was measured with HPLC. Results: Rats receiving methyl-ALA-PDT had a significantly longer survival time compared to the controls. Rats with peritoneal carcinomatosis that received DS+methyl-ALA-PDT+CA had a significantly longer survival time compared to the rats that received DS alone. Some of the rats that received concurrent therapy survived for a prolonged period. Protoporphyrin was highly concentrated in peritoneal metastases, but only small amounts reached major organs in the abdomen. PDT was not found to result in necrosis in the intestines. Conclusions: The results indicated that concurrent therapy consisting of PDT with methyl-ALA and CA is effective at treating peritoneal carcinomatosis resulting from ovarian cancer without damaging organs.
범가자미 , Verasper variegatus 의 생식소 발달단계에 따른 혈중 난황단백전구체 ( vitellogenin ) 와 성 스테로이드 호르몬 변화
백혜자(Hea Ja Baek),한창희(Chang Hee Han),김윤(Yoon Kim),(Katsumi Aida),(Makito Kobayashi) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.5
Annual plasma levels of vitellogenin and sex steroids were investigated in relation to the gonadal development for understanding the endocrine control of reproduction in spotted flounder, Verasper variegatus. The plasma vitellogenin level was highest, 6.36 ㎎/㎖, in November when vitellogenesis was most active. The level, thereafter, decreased to 3.81 ㎎/㎖ in December with the initiation of spawning. On the other hand, estradiol-17β was highest, 2.7 ng/㎖, in December, and rapidly decreased in January when spawning occurred. The decreased level of estradiol-17β, around 0.2 ng/㎖, remained unchanged until May. The profiles of plasma testosterone were similar to those of estradiol-17β in the fish. The plasma 17 α-hydroxyprogesterone level was relatively low throughout the spawning period, but increased slightly with the initiation of ovarian development. In males, the plasma testosterone and 11-ketotestosterone were highest in December when spermiation actively proceeded, but rapidly decreased during the spawning period (January).