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- 저자 : (Katsumi Aida) (검색결과 22 건)
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범가자미, Verasper variegatus의 생식소 발달단계에 따른 혈중 난황단백전구체 (vitellogenin)와 성 스테로이드 호르몬 변화
한창희,會田勝美,김윤,백혜자,小林牧人 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
Annual plasma levels of vitellogenin and sex steroids were investigated in relation to the gonadal development for understanding the endocrine control of reproduction in spotted flounder, Verasper variegatus. The plasma vitellogenin level was highest, 6.36㎎/㎖, in November when vitellogenesis was most active. The level, thereafter, decreased to 3.81㎎/㎖ in December with the initiation of spawning. On the other hand, estradiol-17 β was highest, 2.7ng/㎖, in December, and rapidly decreased in January when spawning occurred. The decreased level of estradiol-17 β, around 0.2ng/㎖, remained unchanged until May. The profiles of plasma testosterone were similar to those of estradiol-17 β in the fish. The Plasma 17 α-hydroxyprogesterone level was relatively low throughout the spawning period, but increased slightly with the initiation of ovarian development. In males, the plasma testosterone and 11- ketotestosterone were highest in December when spermiation actively proceeded, but rapidly decreased during the spawning period (January).
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김대중,김이청,Katsumi Aida 한국통합생물학회 2009 Animal cells and systems Vol.13 No.4
The control mechanism of gonadotropin-releasing hormone (GnRH) on gonadotropin (GTH) release was studied using cultured pituitary cell or cultured whole pituitary obtained from Testosterone (T) treated and control immature rainbow trout. The release of FSH was not changed by salmon type GnRH (sGnRH), chiken-II type (cGnRH-II), GnRH analogue ([des-Gly10D-Ala6] GnRH ethylamide) and GnRH antagonist ([Ac-3, 4-dehydro-Pro1, D-p–F-Phe2, D-Trp3,6] GnRH) in cultured pituitary cells of Ttreated and control fish. Indeed, FSH release was not also altered by sGnRH in cultured whole pituitary. All tested drugs had no effect on the release of LH in both culture systems of control fish. The levels of LH, in contrast, such as the pituitary content, basal release and responsiveness to GnRH were increased by T administration in both culture systems. In addition, the release of LH in response to sGnRH or cGnRH-II induced in a dose-dependent manner from cultured pituitary cells of T-treated fish, but which is not significantly different between in both GnRH at the concentration examined. Indeed, LH release was also increased by sGnRH in cultured whole pituitary of T-treated fish. GnRH antagonist suppressed the release of LH by sGnRH (10−8 M) and GnRH analogue (10−8 M) stimulation in a dose-dependent manner from cultured pituitary cells of Ttreated fish, and which were totally inhibited by 10−7 M GnRH antagonist. These results indicate that the sensitivity of pituitary cells to GnRH is elevated probably through the T treatment, and that GnRH is involved in the regulation of LH release. GnRH-stimulated LH release is inhibited by GnRH antagonist in a dose-dependent manner. The effects of gonadal steroids on FSH levels are less clear. The control mechanism of gonadotropin-releasing hormone (GnRH) on gonadotropin (GTH) release was studied using cultured pituitary cell or cultured whole pituitary obtained from Testosterone (T) treated and control immature rainbow trout. The release of FSH was not changed by salmon type GnRH (sGnRH), chiken-II type (cGnRH-II), GnRH analogue ([des-Gly10D-Ala6] GnRH ethylamide) and GnRH antagonist ([Ac-3, 4-dehydro-Pro1, D-p–F-Phe2, D-Trp3,6] GnRH) in cultured pituitary cells of Ttreated and control fish. Indeed, FSH release was not also altered by sGnRH in cultured whole pituitary. All tested drugs had no effect on the release of LH in both culture systems of control fish. The levels of LH, in contrast, such as the pituitary content, basal release and responsiveness to GnRH were increased by T administration in both culture systems. In addition, the release of LH in response to sGnRH or cGnRH-II induced in a dose-dependent manner from cultured pituitary cells of T-treated fish, but which is not significantly different between in both GnRH at the concentration examined. Indeed, LH release was also increased by sGnRH in cultured whole pituitary of T-treated fish. GnRH antagonist suppressed the release of LH by sGnRH (10−8 M) and GnRH analogue (10−8 M) stimulation in a dose-dependent manner from cultured pituitary cells of Ttreated fish, and which were totally inhibited by 10−7 M GnRH antagonist. These results indicate that the sensitivity of pituitary cells to GnRH is elevated probably through the T treatment, and that GnRH is involved in the regulation of LH release. GnRH-stimulated LH release is inhibited by GnRH antagonist in a dose-dependent manner. The effects of gonadal steroids on FSH levels are less clear.
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Testosterone 처리한 미성숙 무지개송어 뇌하수체의 세포배양계에서 생식소자극호르몬 분비에 대한 Activin의 효과
김대중,한창희,會田勝美 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
The present studies were conducted to evaluate the effects of activin-A on gonadotropins(GTHs) release in testosterone-treated immature rainbow trout, Oncorhynchus mykiss. The administration of testosterone elevated pituitary level of GTH Ⅱ but not of GTH Ⅰ. In this study using primary cultures of dispersed pituitary cells in static incubation, dose-dependent increases in GTHⅡ release was observed in the activin-treated group at day 3 of incubation(long-term incubation), but not at day 1 of incubation (short-term incubation). Dopamine, a potent inhibitor of gonadotropin-releasing hormone (GnRH)-stimulated GTHⅡ release in trout, was only partially effective in decreasing activin-induced GTHⅡ release. Futhermore, salmon GnRH(sGnRH)-stimulated GTHⅡrelease was not potentiated by the pretreatment with activin. However, the control mechanisms of GTHⅠ release by activin and other hormones were not observed in the all tested experiments. The results of these studies support the contention that in contrast with the usual stimulatory effects of activin on GTH release in mammals, activin exerts long-term stimulatory actions on GTHⅡ release in rainbow trout. The control mechanism of GTHⅠrelease, however, is a question that remains to be elucidated.
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Han, Chang Hee,Aida, Katsumi,Kim, Dae Jung 한국수산학회 2000 Fisheries and Aquatic Sciences Vol.3 No.1
We used a mammalian GnRH antagonist,[Ac-3,4-dehydro-Pro¹, D-p-F-Phe², D-Trp^(3.6)]]- GnRH, to examine the details of the salmon type gonadotropin-releasing hormone (sGnRH) and GnRH agonist analog (Des-Gly^(10)[d-Ala^6]- ethylamide GnRH; GnRHa) functions in the control of maturational gonadotropin (GTH II) secretion, in precocious male rainbow trout, in both in vivo and in vitro experiments. In the in vivo study, plasma GTH II levels increased by sGnRH or GnRHa treatment, but the response was more rapid and stronger in the GnRHa treatment group. The increase in GTH II was significantly suppressed by the GnRH antagonist, while the antagonist had no effect on basal GTH II levels in both groups. The GnRH antagonist showed stronger suppression of GTH II levels in the sGnRH treatment fish than in the GnRHa treatment fish. In addition, plasma androgenic steroid hormones (testosterone and 11-ketotestosterone) increased by the sGnRH or GnRHa treatment. The GnRH antagonist significantly inhibited the increases in plasma androgenic steroid hormone levels stimulated by the sGnRH or GnRHa, while the antagonist had no effect on basal androgenic steroid hormone levels in both groups. In the in vitro study, treatment with sGnRH or GnRHa increased GTH II release from the cultured dispersed pituitary cells, but the response was stronger in the GnRHa treatment group. The increase in GTH II release by GnRH was suppressed by adding the GnRH antagonist, dosedependently. On the other hand, basal release of GTH II did not decrease by the GnRH antagonist treatment in both groups. These results suggest that the GnRH antagonist, [Ac-3,4-dehydro-Pro¹, D-p-F-Phe², D-Trp^(3.6)]-GnRH, used in this study is effective in blocking the action of GnRH-induced GTH II release from the pituitary gland both in vivo and in vitro.
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Han, Chang Hee,Katsumi Aida,Kim, Dae Jung 한국수산학회 1999 Fisheries and Aquatic Sciences Vol.2 No.2
The variations of gene expression and pituitary contents of GTH subunits during the ovarian development of masu salmon, Oncorhynchus masou, were investigated. The pituitary GTHs contents was measured by radioimmunoassays (RIAs) using purified GTH subunits and their antibodies. Pituitary contents of GTH Iβ gradually increased from April through August, and reached the maximum in October. On the other hand, pituitary contents of GTH IIβ remained low until August, but they rapidly increased in October. Total RNAs were prepared from pooled pituitaries and the GTH subunits mRNA in pituitaries was quantified by Northern blot hybridization using masu salmon cDNA probes for each GTH subunit. GTH IβmRNA level increased with the progression of ovarian maturity. However, GTH IIβ mRNA was detected only at a more advanced stage, and were extremly high at ovulation. A high levels for GTH α mRNA was detected only at ovulation stage. The synchronous increase in pituitary contents and mRNA levels suggested that ovarian maturity in masu salmon was regulated by both GTH I and GTH II.
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