Acute and Sub-chronic Oral Toxicity Study of Ammonium Persulfate in Spraque-Dawley Rats

Kim, Yong-Soon,Baek, Min-Won,Sung, Jae-Hyuck,Ryu, Hyun-Youl,Kim, Jin-Sik,Cho, Hyun-Sun,Choi, Byung-Gil,Song, Min-Sub,Song, Moon-Yong,Baik, Eun-Ju,Choi, Young-Kuk,Kim, Jong-Kyu,Yu, Il-Je,Song, Kyung-Se Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.3

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 mg/kg body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 mg/kg body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 mg/kg body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

Acute and Sub-chronic Oral Toxicity Study of Ammonium Persulfate in Spraque-Dawley Rats

Yong Soon Kim,Min Won Baek,Jae Hyuck Sung,Hyun Youl Ryu,Jin Sik Kim,Hyun Sun Cho,Byung Gil Choi,Min Sub Song,Moon Yong Song,Eun Ju Baik,Young Kuk Choi,Jong Kyu Kim,Il Je Yu,Kyung Seuk Song 한국독성학회 2009 Toxicological Research Vol.25 No.3

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 ㎎/㎏ body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 ㎎/㎏ body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 ㎎/㎏ body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

열화상카메라를 이용한 블랙아이스 특성 연구

김승준 ( Seung-jun Kim ),윤원섭 ( Won-sub Yoon ),김연규 ( Yeon-kyu Kim ) 한국산업융합학회 2021 한국산업융합학회 논문집 Vol.24 No.6

In this study, a study was conducted to develop a system for predicting/responding to black ice occurring on roads in winter. Tests conditions were studied by making models of cement concrete pavement and asphalt concrete pavement. In order to freeze water on the manufactured model package, an tests was conducted at a temperature below zero using a freezer, and the freezing process was photographed using a thermal imaging camera. Black ice is generated when water is present on the road surface and the temperature is below freezing or the road surface temperature is below the dew point temperature. Under sub-zero conditions, the pavement, water, and ice were classified with a thermal imaging camera. As a result of the tests, it was possible to distinguish with a thermal imaging camera at a temperature below freezing in the same freezer due to the difference in the emissivity of the packaging, water, and ice. In the process of changing from water to ice during the tests, it was analyzed that ice and water were clearly distinguished by the thermal imaging camera due to the difference in emissivity and reflectance, so black ice could be predicted using the thermal imaging camera.

특수체육 전공자들의 윤리적 민감성에 영향을 미치는 요인 분석

김지태 ( Ji Tae Kim ),이원섭 ( Won Sub Lee ) 한국특수체육학회 2012 한국특수체육학회지 Vol.20 No.1

이 연구는 특수체육 전공자들의 인구사회학적 특성에 따른 윤리적 민감성의 차이를 분석하고, 특수체육 전공자들의 개인적 요인과 조직적 요인이 윤리적 민감성에 어떠한 영향을 미치는지를 분석하는 것을 연구의 목적으로 하였다. 연구대상자는 2011년 현재 한국특수체육학회에 등록된 특수체육 전공자들을 모집단으로 하여 유목적 표집법(purposive sampling method)에 의해 추출된 특수체육 전공자 200명 이었다. 이에 수집된 자료 중조사내용 일부가 누락되었거나 불성실하게 답변한 23부를 제외한 총 177부의 자료를 최종 유효 표본으로 사용하였다. 이 연구의 조사도구로는 선행연구에서 조사된 설문지를 수정 및 보완하여 사용하였고, 조사절차는 연구자가 직접 기관을 방문하여 연구대상자들에게 설문에 대한 충분한 안내와 함께 설문참여에 대한 동의를 구한 후, 설문지를 배포하여 자기평가기입법으로 작성하게 한 후 현장에서 즉시 회수하였다. 조사된 자료의 분석방법은 SPSS WIN Ver 18.0 통계프로그램을 사용하여 독립 t-검증(independent t-test), 일원분산분석(one-way ANOVA), 상관관계분석(correlation analysis), 위계적 다중회귀분선(hierarchical multiple regression) 등을 활용하였으며, 그 결과 다음과 같은 결론에 도달하였다. 첫째, 특수체육 전공자들의 인구사회학적 변인 중 연령, 직업 경력, 윤리교육 유/무는 윤리적 민감성에 유의한 차이를 나타냈다. 둘째, 특수체육 전공자들의 개인적 요인과 조직적 요인은 윤리적 민감성에 영향을 미치는 것으로 나타났다. 구체적으로 살펴보면, 개인적 요인으로는 통제위치에서 정(+)적으로 마키아벨리즘에서 부(-)적으로 윤리적 민감성에서 유의한 영향을 미치는 것으로 나타났으며, 조직적 요인은 윤리풍토의 하위요인 중 하나인 원칙주의에서 정(+)적으로 유의한 영향을 미치는 것으로 나타났다. The purpose of this study was to analyze factors that affect ethical sensibility of adapted physical education majors. The population of this study was set to be apecial physical education majors who are registered in Korean Society of Adapted Physical Activity and Exercise (KOSAPE) in 2011. Data collection was done by using widely-used purposive sampling method, from non-probability sampling methods to extract 200 copies in total from adapted physical education majors who have participated in spring and autumn symposium and who are currently working in Korea Sports Association for the Disabled (KOSAD) or related institutions. Here, 177 copies were used as the final valid samples for quantitative research, disregarding 23 incomplete copies. As the research methods, surveys that are revised and complemented ones from the previous researches, research procedures included visiting institutions and asking for agreement after explaining about surveys, and then distributing surveys to be completed by self- administered method and taking them back at sight. For the data analysis of this study, SPSS WIN Ver 18.0 was used with independent t-test, one-way ANOVA, correlation analysis, and hierarchical multiple regression, to reach the following conclusion. First, age, job experiences, and whether they received ethics lessons out of variables of population sociology for Special Physical Education majors have shown meaningful differences on ethical sensibility. Second, individual factors and institutional factors were found out to be affecting ethical sensibility. Upon the individual factors, while locus of control was affecting ethical sensibility positively and Machiavellism was affecting ethical sensibility negatively. And on the institutional factors, principlism, one of the sub-factors of ethical climate, was influencing ethical sensibility.

A pre-dose biomarker Ccrn4l for predicting the susceptibility of cisplatin-induced hepatotoxicity

Da-Bin Hwang,Dong-Hoon Won,Yoo-Sub Shin,Shin-Young Kim,Changuk Kim,Jun-Won Yun 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Cisplatin-induced liver injury is one of the limitations of its use as an anti-cancer drug. In particular, individual differences in sensitivity to drug-induced liver injury (DILI) can lead to life-threatening situations. The purpose of this study is to discover which innate genetic factors determine diversity for susceptibility to DILI. Rats undergo a liver prebiopsy and a 3-week postoperative recovery period prior to cisplatin administration. Two days after administration of cisplatin, hepatotoxicity was confirmed through serum biochemical and histopathological analysis. Based on liver-related biochemical test results (ALT, AST, γGT), rats were sub-grouped into the Susceptible (top five) or Resistant (bottom five) group for RNA sequencing using the pre-collected liver samples. As a result, pre-dose 161 genes were differentially expressed between the susceptible and resistant groups. Among them, Ccrn4l, a clock-controlled gene included in the "rhythmic process", was inherently low in gene expression before drug administration in both cisplatin- and acetaminophen-susceptible animals. In addition, in the susceptible group, the innately low expression level of Ccrn4l was maintained even after cisplatin treatment with decreased antioxidants, increased nitration, and apoptosis. When checking the correlation between Ccrn4l, antioxidant catalase, and mitochondrial RNA in the liver, it was confirmed that they showed similar mRNA expression patterns according to individual circadian variation. Surprisingly, Ccrn4l knockdown WB-F344 cells prompted cisplatin-induced mitochondrial dysfunction, apoptosis, and decreased catalase activity. In conclusion, individual innate hepatic Ccrn4l level may be a novel factor affecting cisplatin-induced hepatotoxicity susceptibility by modulating mitochondrial and antioxidant functions.

콜롬비아 소가모스에서 미세먼지 감축 시나리오 분석

박일수(Park, Il-Soo),장유운(Jang, Yu-Woon),하상섭(Ha, Sang-Sub),장수환(Jang, Su-Hwan),정경원(Chung, Kyung-Won),김혜원(Kim, Hye-Won),김희선(Kim, Hee-Sun) 한국외국어대학교 중남미연구소 2016 중남미연구 Vol.35 No.2

콜롬비아 소가모스 지역에서 미세먼지 감축 시나리오를 TAPM 모델을 활용하여 도출하고 평가하였다. 시나리오 1의 제철소 및 금속 등 중공업 오염원을 대상으로 미세먼지 방지 기술을 이용하여 배출량을 80% 감축하는 방안에서는 도시 중심 평균 농도는 6.5 μg m-3로 1.5 % 감소하였고, 모델영역에서 최고 평균농도는 387 μg m-3 로 변동 없었다. 시나리오 2의 벽돌 및 석회공장 등을 대상으로 연료를 석탄에서 코크스로 교체하여 90 % 감축하는 방안에서는 도시 중심 평균 농도는 4.5 μg m-3로 31.8%, 모델영역에서 최고 평균농도는 111 μg m-3 로 71.6 % 감소하였다. 시나리오 1과 시나리오 2를 결합한 시나리오 3에서는 도시 중심에서 평균 농도는 4.3 μg m-3로 34.8 %, 모델영역에서 최고 평균농도는 110 μg m-3 로 71.7 % 감소하였다. 소가모스 지역에서 대기환경을 개선하는 로드맵 수립에는 수공업에서 주로 사용되는 석탄 연료를 청정연료인 코크스로 교체하는 방안이 최우선으로 고려되어야 하겠다. 이후 미세먼지 방지 시설을 굴뚝에 설치하는 방안도 차선책으로 고려하여 환경과 경제가 선 순환하는 녹색성장관점에서 장기적인 미세먼지 개선 마스터 플랜이 수립되어야 하겠다. Reduction scenarios for PM10 concentration in Sogamoso, Colombia were analyzed using the TAPM( The Air Pollution Model). The average concentration of PM10 in the downtown area was 6.5 μg m-3, a reduction of 1.5 %. The maximum average concentration in the model domain was 387 μg m-3 without any reduction in scenarios 1 which the PM10 emissions from heavy industries such as iron and metal was reduced by 80 % using the prevention technology. In scenario 2 which the PM10 emissions from the brick and lime industries was reduced by 90 % substituting coke (a cleaner fuel) for coal, the average concentration of PM10 in the downtown area was 4.5 μg m-3, a reduction of 31.8 % , the maximum average concentration in the model domain was 111 μg m-3 , a reduction of 71.6 %. In scenario 3 which the scenarios 1 and 2 were combined, the average concentration of PM10 in the downtown area was 4.3 μg m-3, a reduction of 34.8 % and the maximum average concentration was 110 μg m-3, a reduction of 71.7 %. To put forward a road map for the reduction of PM10 in the Sogamos region, substituting coke for coal in manual industries should have priority over reduction policies. Also, it is highly recommended that a longterm master plan for reducing PM10 concentrations should be established with a focus on environmentally-friendly development and future green growth policy with a win-win relationship between economy and environment.

촉매합성된 다중벽 탄소나노튜브의 급냉 열처리법에 의한 정제

김용환,서용기,정우창,정원섭 대한금속ᆞ재료학회 2006 대한금속·재료학회지 Vol.44 No.3

태권도 앞밀어차기 동작의 운동역학적 분석

김원섭,신성휴 한국운동역학회 2000 한국운동역학회지 Vol.9 No.2

The purpose of this study is firstly to find out the characteristics of motions through the analysis of temporal factor of phase, velocity, angular, angular velocity of the segment, ground of reaction force and the moment of the lower data in the Abmilechagi Taekwon-do and second to contribute to the systematic technical coaching on the next motion, the offer of basic data and the ability of winning a match. In order to accomplish this purpose the analytical research with the three national team players was needed and the following results were found: 1. The total amount of time in carrying out the motion of the Abmilechagi is 0.64±0.03 seconds on the average though, there seems to be no difference between the three persons and it appeared that it is better to shorten take-off than to make the next step longer. 2. The distance of movement of the center of gravity of human body to the left and right sides is from 5.73±4.55cm on the average in take-off and shows relatively a big difference in the movement of the center of body to the two sides while kicking. The movement of the center of gravity of human body into forward and backward 44.63±30.34cm on the average in kicking, and the center of gravity of human body tends to more to forward while kicking. The distance of the center of gravity of human body to upward and lower separately reveals the distance of 10.50±7.45cm and of 10.43±10.14cm on the average. As a result, the power of moving to upward while raising the leg is being transmitted to the kicking moment. 3. The distance of the center of foot to left and right sides in kicking motion is 19.5±15.61cm in lifting up moment, and appears to be ineffective in contrast to the effects of other moments. The distance of the movement to upward and power shows that there is little change from raising to kicking. 4. There's not much change in speed in the movement of the center of gravity of human body to four sides. However, the change of speed in forward and backward motions is noticeable from the moment of raising the foot to the end of kicking. Therefore, So, it is discovered that to kick so fast and move the foot quickly are closely related to the use of the forward movement speed of the center of gravity of human body. 5. The speed change of kicking foot to forward side appears faster than the there moments and shows so different results than the there movement. 6. In he angle of knee in kicking, the smallest angle is 66.8±69.4 on the average, and is 156.5±10.95 during the time of impact on the average. 7. The bending angular velocity hip joints marks 734.0±175.55deg/s in the moment of smallest angle of knee in the next kicking, and proves that the velocity of turning round increases rapidly so as to shorten the angle of knee as much as possible. Angular velocity of extension in the moment of impact marks 196.3±29.10deg/s and appears to push and kick using extension of hip joints. angular velocity of flexion of knee joints is 283.8±3.86deg/s on the average, and angular velocity of flexion of hip joints is faster than angular velocity of flexion of knee joints. 8. Ground of reaction force of the standing leg is from 198(0.30BW) - 528.4(0.90BW) 9. The muscle moment of flexion of hip joints shows that the smallest knee angle of kicking leg is 12.9±4.87Nm on the average, and angular velocity of extension of knee joints is 2.6±2.38Nm in impact time on the average proving the rapidity of angular velocity of extension in impact.

고분자-금속착물(Ⅳ)ㆍPolypropyleneimine-Ni(Ⅱ) 착물의 촉매활성도

金東洙,曺泰燮,石元慶,朴正學 嶺南大學校附設 基礎科學硏究所 1982 基礎科學硏究 Vol.2 No.-

이상의 실험결과로 보아 중합체 리간드 사슬에 치환기가 있거나, 분자량이 큰 중합체 리간드는 금속착물의 촉매작용을 증가시키는 것을 알 수 있으며, 치환기의 크기를 변화시키거나 증합체의 분자량을 조절함으로써 금속착물의 촉매작용을 조절할 수 있다는 것을 알 수 있다. 메틸기보다 더 큰 알킬기(에틸,n-프로필 등)를 가졌거나 여러가지 분자량을 가진 중합체 리간드로 된 금속착물의 합성 및 촉매활성도 조사가 현재 진행중에 있으며 이 실험결과는 이미 합성된 PPI 리간드의 분자량과 함께 다음의 완보에 발표될 것이다.