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Lee, Seunghan,Lee, Sunhee,Yoon, Yeo Joon,Lee, Jinwon Humana Press 2013 Applied biochemistry and biotechnology Vol.169 No.2
<P>The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, are Escherichia coli homologues and function in the elongation cycle of fatty acid biosynthesis. FabB (fabB), an activator of FAS, is a β-oxoacyl-ACP synthase, which catalyzes the addition of acyl-ACP to malonyl-ACP to generate β-oxoacyl-ACP. FabF (fabF) participates at the same step as FabB in the elongation cycle and is structurally and functionally similar to FabB. Hence, we attempted to see if FabF was an activator of FAS, like FabB, with the rationale that these two enzymes have striking similarities. FabF exhibits thermal regulation in that enzyme activity increases at lower temperatures. To confirm its role as an activator of FAS, fabF was overexpressed solely or with other genes in the elongation cycle through biochemical engineering. The fabF recombinants were cultured at different temperatures, resulting in increased total and unsaturated fatty acid accumulation in all the recombinants, compared to wild type, at lower temperatures.</P>
사료내 약용작물 부산물이 가숭어(Liza haematocheila)의 성장, 혈액성상, 면역반응에 미치는 영향
이봉주 ( Bong-joo Lee ),김민기 ( Min-gi Kim ),허상우 ( Sang-woo Hur ),이승형 ( Seunghyung Lee ),이진혁 ( Jinhyeok Lee ),김현종 ( Hyeon Jong Kim ),김이경 ( Yikyung Kim ),이승한 ( Seunghan Lee ),김강웅 ( Kang-woong Kim ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.4
By-products produced from medicinal plants (MP) were evaluated as feed additives on growth performance, blood chemistry, and immune responses of the juvenile red lip mullet Liza haematocheila. A commercial diet was used as the control. Four experimental diets were prepared by combining 0.5% of MPs such as Panax ginseng (PG), Schisandra chinensis (SC), Angelica gigas (AG), and Rehmannia glutinosa (RG), via water absorption, to the control diet. Triplicate groups of fish averaging 61.8 g were fed one of experimental diets to apparent satiation for 8 weeks. Dietary supplementation of MPs did not affect the growth performance, biological indices and whole body composition of fish compared to the control. The fish fed with AG diet had an increased weight gain, and specific growth rate compared to fish in SC group. Decreased level of plasma glucose was observed in fish fed diet containing MPs. Lysozyme activity was significantly increased in the fish fed AG diet compared to that of fish in PG group. These results indicate that dietary supplementation of Angelica gigas may enhance growth performance and immune responses in juvenile red lip mullet.
Lee, Seunghan,Katya, Kumar,Park, Youngjin,Won, Seonghun,Seong, Minji,hamidoghli, Ali,Bai, Sungchul C. Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.61 No.-
<P><B>Abstract</B></P> <P>The current experiment was conducted to evaluate and compare the efficacy of two different probiotics <I>Bacillus subtilis</I> WB60 and <I>Lactobacillus plantarum</I> KCTC3928 in diet of Japanese eel, <I>Anguilla japonica</I>. Seven experimental diets were formulated to contain no probiotics (CON), three graded levels of <I>B. subtilis</I> at 10<SUP>6</SUP> (BS<SUB>1</SUB>), 10<SUP>7</SUP> (BS<SUB>2</SUB>), 10<SUP>8</SUP> (BS<SUB>3</SUB>) and <I>L. plantarum</I> at 10<SUP>6</SUP> (LP<SUB>1</SUB>), 10<SUP>7</SUP> (LP<SUB>2</SUB>), 10<SUP>8</SUP> (LP<SUB>3</SUB>) CFU/g diet. Twenty fish averaging 8.29 ± 0.06 g were distributed in to 21 aquaria and were randomly assigned to one of the experimental diets in triplicate groups. Average weight gain (WG), feed efficiency (FE), and protein efficiency ratio (PER) of fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> (BS<SUB>2</SUB>) and 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet were significantly higher than those of fish fed other experimental diets (<I>P</I> < 0.05). Nonspecific enzymatic activities including lysozyme, superoxide dismutase (SOD), myeloperoxidase (MPO) from fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> (BS<SUB>2</SUB>) and 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet were detected to be significantly higher than that from fish fed CON diet (<I>P</I> < 0.05). Whereas, level of intestine glyceraldehyde-3-phosphate dehydrogenase (GAPDH), heat shock protein 70, 90 (HSP70, 90) and immunoglobulin (IgM) from fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> and 10<SUP>8</SUP> CFU/g diet were recorded to be significantly higher than those of fish fed other experimental diets (<I>P</I> < 0.05). Parameters for intestinal morphology and histology suggested a healthier gut for the fish fed10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet as compared to other treatment groups. Whereas, results from the disease challenge test with bacteria <I>Vibrio angulillarum</I> showed significantly lower survival rate for fish fed CON diet than those of fish fed other experimental diets. Therefore, these results indicated that oral supplement of <I>B. subtilis</I> at 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet could be a more effective source of probiotic compared to <I>L. plantarum</I> in Japanese eel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Preliminary report comparing two probiotics, <I>Bacillus subtilis</I> WB60 and <I>Lactobacillus plantarum</I> KCTC3928. </LI> <LI> Differences on the growth performance, survival rate, immune parameters and gut morphology were clearly observed. </LI> <LI> Oral administration of bacteria spp. <I>B. subtilis</I> at 10<SUP>7</SUP> CFU/g kg<SUP>−</SUP> <SUP>1</SUP> diet could be a more promising source and dose compared to probiotic <I>L. plantarum</I> in freshwater aquaculture. </LI> </UL> </P>
Lee, Seunghan,Katya, Kumar,Hamidoghli, Ali,Hong, Jeongwhui,Kim, Dae-Jung,Bai, Sungchul C. Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.83 No.-
<P><B>Abstract</B></P> <P>This study evaluated the synergistic effects of dietary <I>Bacillus subtilis</I> WB60 and mannanoligosaccharide (MOS) in juvenile Japanese eel, <I>Anguilla japonica</I>. Seven treatment diets were formulated to contain three different levels of <I>B. subtilis</I> (0.0, 0.5, and 1.0 × 10<SUP>7</SUP> CFU/g diet denoted as BS<SUB>0</SUB>, BS<SUB>0.5</SUB>, and BS<SUB>1</SUB>, respectively) with two MOS levels (0 and 5 g/kg diet denoted as M<SUB>0</SUB> and M<SUB>5</SUB>, respectively), and one diet with oxytetracycline (OTC) at 5 g/kg diet. Each diet (BS<SUB>0</SUB>M<SUB>0</SUB> (CON), BS<SUB>0</SUB>M<SUB>5</SUB>, BS<SUB>0.5</SUB>M<SUB>0,</SUB> BS<SUB>0.5</SUB>M<SUB>5,</SUB> BS<SUB>1</SUB>M<SUB>0,</SUB> BS<SUB>1</SUB>M<SUB>5</SUB>, and OTC) was fed to triplicate groups of 20 fish averaging 9.00 ± 0.11 g (mean ± SD) for eight weeks. Average weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets were significantly higher than those of fish fed CON, BS<SUB>0.5</SUB>M<SUB>0</SUB> and OTC diets (<I>P</I> < 0.05). Significant increases in the nonspecific enzymatic activities (e.g., lysozyme and myeloperoxidase) were detected from fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB>, BS<SUB>1</SUB>M<SUB>5</SUB>, and OTC diets compared to the CON, BS<SUB>0.5</SUB>M<SUB>0</SUB>, and BS<SUB>0</SUB>M<SUB>5</SUB> diets (<I>P</I> < 0.05). Whereas, immunoglobulin M expressions were recorded significantly higher for fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets compared to those of fish fed the other diets (<I>P</I> < 0.05). Also, heat shock protein 70 mRNA levels of fish fed BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets were significantly higher than those of fish fed the CON diet (<I>P</I> < 0.05). Histological observations of the intestinal morphology showed healthier gut for fish fed BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets than those fed CON, BS<SUB>0</SUB>M<SUB>5</SUB>, and OTC diets. Additionally, resistance to bacterial challenge with <I>Vibrio anguillarum</I> was recorded significantly lower for fish fed the CON diet than those fed other diets (<I>P</I> > 0.05). Therefore, the results for growth performance, non-specific immune responses, intestinal morphology, and disease resistance demonstrated that supplementation of <I>B. subtilis</I> at 0.5 × 10<SUP>7</SUP> CFU/g diet and mannanoligosaccharide at 5 g/kg diet could have beneficial synergistic effects in Japanese eel. The isolated probiotic from eel and the selected prebiotic could lead to the development of a specific and potential synbiotic in Japanese eel aquaculture.</P> <P><B>Highlights</B></P> <P> <UL> <LI> •Higher growth and immunity could be achieved by the administration of <I>Busilus subitlis</I> and mannanoligosaccharide. </LI> <LI> •Dietary synbiotic could have more benefits than probiotic and prebiotic alone. </LI> <LI> •Specific synbiotic has the potential to replace chemical antibiotics in Japanese eel aquaculture. </LI> </UL> </P>