Postweaning Multisystemic Wasting Syndrome (PMWS)에 이환된 자돈에 대해 Porcine circovirus 2 Rep 유전자 검출에 관한 연구

이용훈,김지영,楚佳奇,서상희,장경수,김명철,박창식,전무형 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Postweaning multisystemic wasting syndrome (PMWS) is a new emerging disease in swine herds world wide, and porcine circovirus type 2 (PCV2) is known as a major cause of PMWS. In this study, the prevalence of PCV2 Rep gene was examined for the serum, nasal swabs and feces collected from 30 heads of piglets affected with PMWS and 10 heads of normal healthy piglets using polymerase chain reaction (PCR). The diagnostic efficiency of the PCR was evaluated for different types of samples. Rep gene of the isolate (PCV2 S1) was then sequenced. In the group of PMWS-affected piglets, the prevalence of PCR-positive were 70.0% in sera, 33.3% in nasal swabs and 0% in feces. In the normal healthy group, the PCR-positive rates of sera, nasal swabs and feces were 30%, 20% and 0%, respectively. Seven piglets (17.5%) out of 40 showed the simultaneously positive results in both of sera and nasal swabs. The nucleotide sequence homology of Rep gene of PCV2 S1 as compared with the reference strain of PCV2 (GenBank accesson no.AF027217) was 99.3%.

Overlay 기반의 WiBro와 WLAN 이종망 연동을 위한 Vertical 핸드오버 구현 및 성능 측정에 관한 연구

이재호,류형근,강선무,이재용,김승천 한국정보사회진흥원 2007 정보화정책 Vol.14 No.3

유비쿼터스 사회를 위한 미래 네트워크는 다양한 이종 무선망이 혼합된 형태가 될 것으로 예상되고 있다. 본 논문에서는 개별 무선망의 특징을 최대한 활용하여 1,270,000㎡ 면적의 KAIST(Korea Advanced Institute of Science and Technology) 대학교 캠퍼스에 IEEE 802.16e/WiBro와 IEEE 802.11/WLAN 를 복합적으로 설치하여 건물외부에서는 WiBro, 건물내부에서는 WLAN으로 자동 스위칭 되는 Vertical 핸드오버 System을 구축하고 이동 성능을 분석하였다. 이종망 통합 과정에서 제조사 및 통신사업자의 사정으로 인하여 Mobile IP를 지원하지 않는 라우터들이 존재하였고, 특히 현재 WiBro에 사용되는 ACR(Access Control Router)은 Mobile IP를 지원하지 않기 때문에 이를 해결하기 위하여 Overlay 기반의 Mobility 기법을 적용하였다. 본 연구에서는 실제로 대규모 통신망을 구현하고, 이에 대한 성능시험을 시행한 후 handover delay 시간 분석을 통한 현장 적용 가능성 및 개선 필요사항을 도출하였다. 그 결과 이종망간 핸드오버 시간은 평균 2.58초로써 일반 데이터 서비스는 이종망간 연속성이 보장됨을 확인하였고, 음성∙영상과 같은 실시간 서비스는 중간에 지연시간이 발생하나 응용서비스 세션 자체는 유지됨을 확인하였다. The future network is forecast with the fact that the form where the various heterogeneous wireless networks. In this paper we build both IEEE 802.16e/WiBro and IEEE 802.11/WLAN network on the KAIST(Korea Advanced Institute of Science and Technology) campus(1,270,000m2) as well as established the vertical handover system which supported WLAN in the building and WiBro outside the building. A Mobile IP is considered for the efficient vertical mobility function in IEEE 802.16/WiBro and IEEE 802.11/WLAN environment. However, during the deployment of WiBro, only some of the ACRs(Access Control Router) can support the Mobile IP function. Therefore, WiBro network can not support the mobility function for the mobile node. In this paper, we implement an overlay-based Mobile IP function and measure the performance through a field trial. Moreover, we evaluate the possibility of commercial deployment and suggest areas that need to be improved. The average handover time between WiBro and WLAN was 2.58 seconds. As a result, the user application session maintained but real time applications were delayed during the handover time.

2 Parameter Criterion을 이용한 안정성 평가에 관한 연구

이종형,유덕상,박무수 國立金烏工科大學校附設生産技術硏究所 1997 産業技術開發硏究 Vol.13 No.-

In this paper, the following results about character stics of surface fatigue crack are obtained by △p decreasing experiment and crack generation using SPPS 42 of pipe-use carbon steel. The △Kthe of surface crack is smaller than the △Kth of through crack. Therefore, if the △Kth of through crack is used as defect estimation, it becomes dangerous. The validity of Engineering safety is represented establishing fatigue fracture estimation diagram by the 2 parameter criterion of threshold and fatigue limit.

컴퓨터 모델러를 활용한 귀금속 제품디자인 실체화 프로세스에 관한 연구

이무형 啓明專門大學 産業開發硏究所 2002 啓明硏究論叢 Vol.20 No.1

산업기술의 급속한 발전에 따라 제품개발 환경이 크게 변화하고 있다. 이러한 변화들은 기존의 제조방법에 새로운 변모를 요구하고 있다. 최근 컴퓨터 기술이 다양화됨에 따라 귀금속 산업디자인 분야에도 수용해야 될 기술이 늘어나고 있으며 이에 맞는 디자인 프로세스 도입 또한 절실한 상태이다. 본 연구는 귀금속 산업디자인 단계에 컴퓨터디자인을 실체화하는 프로세스 구축을 제안하고 문제점을 파악하였고 개선방안을 제시하는 것을 목적으로 한다. 이를 위해 디자인 프로세스의 이론적 근거를 마련하였고, 귀금속 제품 프로세스의 현실적 상황과 컴퓨터 활용에 관련된 현황을 조사하였다. 그리고 컴퓨터를 이용한 디자인 실체화 프로세스 구축을 위해 컴퓨터 모델러 샘플을 직접 제작하였고, 문제점을 분석하였다. 이러한 것을 토대로 하여 새로운 제품개발 환경에 맞는 귀금속디자인 실체화 프로세스의 이론적 근거를 제시하였으며 결론을 도출하였다. The purpose of this research is to suggest a way to set up a process to practise computer design at the stage of jewelry product design and find its problems for the better way. To achieve this purpose, it is required to prepare the theoretical background of design process and investigate the current stage of jewelry product process and its computer-use. In addition, in order to practise the design process through computer, it is necessary to make a computer-modeler sample and examine its problems. On the base of the findings, it is expected to put forward the theories to contrive the jewelry design process fitting for the new environments of product development and draw a conclusion.

忠南地域 屠畜豚의 肺病變으로부터 分離한 Actinobacillus pleuropneumoniae의 生物學的 및 免疫學的 特性

이종훈,장경수,장치훈,김일택,정용성,김귀현,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A. pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of A. pleuropneumoniae isolates were investigated. In addition, outer membrane protein(OMP) of the isolates were extracted to determine its properties and immunogenicity in both mice and piglets. Of 3,395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A. pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A. pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were 9.2×10^7CFU and 2.8×10^7CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low in heart blood and tracheas. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2(5.4×10^8 - 5.4×10^6CFU) abd serovar 5(2.8×10^8 - 2.8×10^6CFU) was 20-40% and 40-80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found i the peaks 1 and 2. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70-80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.

동물원에서 사육중인 Japanese Macaque (Macaca fuscata)에서 Zoletil 마취에 관한 연구

이재일,이수진,홍성혁,신남식,김덕환,박창식,전무형,조종민,김영석,김명철 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

To evaluate the effects of Zoletil (tiletamine-zolazepam) that have been widely used for the chemical restraint and anesthesia of primates, on physiologic alteration, blood gas analysis and anesthetic duration in the Japanese macaque(Macaca fuscata), this study was carried out. Zoleril was administered by intramuscular injection, Evaluation of temperature, heart rate, respiration rate and blood gas analysis were performed before administration ancl at 1,10, 20, 30, 40, 50 and 60 min after administration, and induction and maintenance time was recorded. There was no significant difference in heart rate, PCO₂PO₂after Zolcril administration and temperature, respration rate, pH were significant difference compared with those of before administration, The induction time was 2.5±l.Omin and maintenance time was 86±23.2min. It was considered that Zoletil could he usefully used for the sedation and immobilization of Japanese macaque reared in zoological garden.

ARTCLAY를 이용한 BROOCH DESIGN에 대한 연구

이무형 啓明專門大學 産業開發硏究所 1999 啓明硏究論叢 Vol.17 No.2

현대인의 미적 욕구를 충족시키기 위한 장신구 디자인 개발문제는 공예산업적 측면에서 뿐만 아니라 문화적인 측면에서도 매우 중요한 과제이다. 본 연구는 이러한 중요성에 입각해 경제적이고도 합리적인 아트클래이 브로치 디자인을 개발하는데 목적을 두고 있다. 이것을 위하여 생산된 아트클래이 제품의 디자인 특성을 분석하고 아트클래이 재료 및 기법에 대하여 조사하였다. 이러한 관련자료를 통하여 본 연구가 추구하는 디자인의 방향을 설정하였으며 이를 토대로 하여 代案을 구성하였다. 그리고 대안의 장·단점을 분석 한 다음 이상적인 것을 선택해 試案으로 제시하였다. 본 연구를 통한 결과는 시대적 요구에 적합한 공예품 제작에 도움이 될 것으로 여겨지며 향후의 문화 발전에도 기여될 것으로 생각한다. The study was about developing brooch design using artclay material. The purposes of the study were to research the historical background of the brooch(accessories), how the artday product is distributed, and to explain the characteristics of the artclay product. Through the material concerned, I could decide upon the direction of the study, and put forward an alternative for effective artclay brooch design. Analyzing the merits and weak points, I selected the ideal design and manufactured a brooch for display. The study is useful for developing craft work which is appealing to people's needs of the day. I expect the study will be helpful for improving culture.

腎臟組織에서 백강잠 抽出物의 抗酸化 作用에 關한 硏究

李武炯,尹哲浩,鄭智天 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.7 No.1

白彊蠶 추출물이 신장조직에서 반응성 산소기들에 의한 지질의 과산화와 세포 손상을 방지하는지를 관찰하기 위하여 정상조직과 t-BHP를 처리한 조직에서 白彊蠶 추출물의 효과를 조사하였다. 지질의 과산화와 LDH 유출은 t-BHP의 농도에 비례하여 증가되었으나, 白彊蠶 추출물의 첨가로 유의하게 억제되었다. 그리고, t-BHP에 의한 지질의 과산화 및 LDH 유출은 白彊蠶 추출물의 농도에 비례하여 억제되었다. 또한, 白彊蠶 추출물은 oxidant를 처리하지 않은 정상조직에서도 지질의 과산화 및 LDH 유출을 유의성 있게 감소시켰다. 그리고, catalase 활성에는 영향이 없는 반면, 정상 조직과 t-BHP를 처리한 조직에서는 glutathione peroxidase 활성을 유의성 있게 증가시켰다. 한편, 반응성 산소기의 발생은 白彊蠶 추출물의 농도에 비례하예 억제되었다. 이러한 결과로 볼 때, 白彊蠶 추출물은 신장 조직내 항산화 효소의 활성을 증가시킴과 동시에 직접 반응성 산소기의 발생을 억제하므로 신장 조직에서 지질의 과산화와 세표손상을 방지하는 효과가 있을 것으로 여겨진다. This study was undertaken to determine whether Bombycis Corpus extract (Bom) has antioxidant action. Kidney tissues were exposed to t-butylhydroperoxide (t-BHP) to induce oxidative stress. Lillid peroxidation was estimated by measurimg rnalondialdehyde, a product of lipid peroxidation, and cell injury was estimated by measuring lactate dehydrogenase (LDH) release in rabbit renal cortical slices, t-BHP increased lipid peroxidation and LDH release in a dose-dependent manner over the concentration range of 0.1-1 mM. Such effects of t-BHP on lipid peroxidase and LDH release were prevented by 0.5% Bom. When tissues were treated with t-BHP in the presence of various concentration of Bom, lipid peroxidation and LDH release were dose-dependently inhibited by Bom. Bom at 1 and 2% concentrations inhibited lipid peroxidation and LDH release in normal tissues. Bom at 2% concentration increased glutathione peroxidase activity in tissues treated or untreated with 1.0 mM t-BHP. However, catalase activity was not altered by addition of Bom. Bom inhibited generation of reactive oxygen species. These results indicate that Born inhibits lipid peroxidation and cell injury in tissues treated with or without oxidant and effect is, at Ieast in part, attributed to increased activity of glutathione peroxidase and a direct sacvenging action.

생태단지계획기법의 수립에 관한 기초연구

이영무,문형택 홍익대학교환경개발연구원 2000 환경개발연구논문집 Vol.5 No.-

The purpose of this thesis is the establishment of basic guidelines to create an ecologically sound apartment complex. The case studies of foreign and korean eco-site planning lead to the extraction of basic principles that should be applied in realization of ecologically healthy apartments. The guidelines consist of six categories and these principles are desired to apply to the planning process of future apartment complex. The first is the application of environment friendly housing construction techniques. This includes solarium, indoor garden, balcony garden and greening of apartment outer walls. The second is the recycling of resources. It involves recycling of wastes, compost making, water retention and purification. The third is the creation of biotope. It means the designing of fish pond, stream and paving with permeable material. The insects such as butterflies and dragonflies are encouraged to settle in the biotope. The fourth involves the creation of green network within the complex. It consists of various gardens and eco-corridors which connect the greeneries. The system eventually leads to invitation of not only insects and birds but also small mammals like squirrels. The fifth is the harmonizing of the apartments with the existing topography. It means the preservation of the forst, minimizing the soil removal and positioning of buildings to fit the natural energy. The sixth includes active and passive harnessing of sun, wind and geo-thermal energy extraction. Above techniques are recommended to be utilized actively in the planning process of healthier apartment construction to achieve the goal of UN resolution of Rio de Janeiro in 1992 which states the 'Environmentally Sound and Sustainable Development' in order to continue the symbiosis of human race and the nature.

家兎網膜 神經節細胞의 總數와 分布에 關한 硏究

李亨周,金武剛 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

The number and distribution of the retinal ganglion cells in the rabbit was determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. The total number of retinal ganglion cells was estimated to be 903,600 in retina ranging from 641㎟ in total area. 2. The number of alpha type ganglion ceils(above 15 microns) was 118,000 in the retina of rabbit and constituted 13% of the total cells. 3. The visual streak was recognized above 2.5mm from optic disc and ganglion cell density drops off rapidly above and below the visual streak. 4. Area centralis(8,300/㎟) was recognized at the area of 10mm temporally, from the point of 2.5mm superior to the optic disc. 5. The relative frequency of the alpha type ganglion cells was higher in the superior-nasal quadrant than in other region of the retina.