Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

일개 농촌지역의 지역사회 영양정보에 관한 조사연구 : 경기도 남양주군 수동면을 중심으로 Su-Dong Myun, Nam Yang-Ju Gun, Kyung-Gi Do

계난이,권계숙,송미희,이연주 이화여자대학교 의과대학 1988 梨花醫學誌 Vol.- No.20

From September to November in 1987, the study was conducted a survey of Su-Dong Myun, Nam Yang-Ju Gun, Kyung-Gi Do, a comprehensive rural care demonstration area of Ewha Womans University. The purpose of this survey has been to examine into community nutrition informathrough preventive medicine training of sophomores of medical course. The result were as follows 1) Female respondent was 88.2% of all whose age was the forties and fifties at the highest rate of 22.6% respectively and whose educational level was uneducated at rate of 31.2%. 2) Agriculture(66.7%) was the common householder's occupation whose main income was from their own farm products(67.7%). 3) A family of five(24.7%) and two generations(e.g parent and children) (55.9%) was the most common family. 4) The housewife(98.9%) was the most common cook and 35.5% of all cook was not able have sufficient time to prepare for their meal. 5) On food purchase, main food was largely self provided(65.6%) and subsidiary food with self-provision and outside purchase(51.6%). 6) On the self sufficient food rice(77.4%) was most common as main food and vegetable(81.7%) as subdiary food. 7) On the frequency of purchasing food, main food was about 2-3 times a month, subsidiary food 1-3 times a week. On the market place, main food mainly self sufficient(59.1%) and subsidiary food was largely purchased from the peddlers and market on the move(35.5%) 8) On the means of transportation of purchased food, main food was conveyed by bus or another kinds of vehicles and subsidiary food(52.7%) was in the same way. 9) On the consumer's difficulty in purchasing food, meat and fish were rated 76.3% and vegetable 78.5%, 76.3% of all consumer had difficulty in purchasing meat and fish, 78.5% in purchasing grocery. 10) 94.6% was in possession of electric refrierator and 89.2% possessed their own wells. 11) The respondent had such fuels for cooking as propanegas(66.6%), wood(18.3%) briquet(10.8%). 12) the present state of nutrition was that the mean points was 2.7 in the group 1, 2.2 in the group 2, 3.9 points in the group 3, 2.8 points in the group 4, 3.1 points in group 5 and 2.7 points in the group 6. 13) Most families' favorite food was kimchi or kimchi soup and distasteful was meat including dog meat. 14) 48.4% of all respondents took no interest in taking in adequate nutrition owing to the lack of money and time to spare.

수술적으로 치료한 통증을 동반한 흉추부의 후외상성 Schmorl씨 결절 : 2례 보고 Report of Two Cases

신병준,이재철,송화용,정석봉,권계원,조영일,김연일 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Study Design : Two patients who had painful Schmorl's node at thoracic level from acute trauma were adopted and taken operation for its treatment Objectives : To document the effect of operation for the treatment of painful Schmorl's node at thoracic level summary of background data : Schrmorl's nodes are common spinal lesion generally believed to be asymptomatic, but in some cases there were painful symptom by Schmorl's node. This case report is to explain the effect of operative treatment for the painful Schmorl's node. Methods : Two patients were adopted who had traumatic painful Schmorl's node at thoracic level for our case report. They were treated conservative methods at first but it's effect were poor, so they were taken anterior decompression and fusion, and checked back pain symptom. Result : In all two patients, the symptom of back pain by Schmorl's nodes at thoracic level was almost subsided. conclusion : For the treatment of traumatic painful Schmorl's node, most of surgeon says that conservative treatments were treatment of choice, but operative treatments are also benefit to subsidence of back pain by Schmorl's node.

자외선 조사에 의한 인체 각질형성세포 세포고사 방어인자에 관한 연구

박수홍,박준홍,이종석,황규왕,김계정 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Back ground: Human skin is continuously exposed to UV irradiation. Ultraviolet irradiation of human skin cause sunburn cell which is relevant to the apoptosis of keratinocytes. In the epidermis, apoptosis inducing factors and anti-apoptotic factors probably exist to maintain the integrity of keratinocytes. Objective: The purpose of this study was to investigate the exsistence of apoptosis inducing factors and anti-apoptotic defence factors by evaluation of UV induced apoptosis in cultured human keratinocyte and other keratinocyte cell lines(A-431 cells, KB cells) and its susceptibility of UV induced apoptosis in various conditions. Method: In this study, the percentages of apoptosis, necrosis and cell viability of irradiated human keratinocytes and othe keratinocyte cell lines by MMT assay and AO/EO stain. The percentages of those were measured before UVB irradiation and 8, 24, 48 hours after UVB irradiation. Also, the same evaluations were performed with irradiated human keratinocytes cultured without growth factor and with enough growth factors, both results were compared with each other. And the effect of cycloheximide, a protein synthesis inhibitor was evaluated. Also that of aurintricarboxylic acid(ATA), an inhibitor of endonucleases which play an important role in inducing apoptosis of human keratinocytes was evaluated. Result: Human keratinocytes and other keratinocyte cell lines(A-431 cells, KB cells) were cultured in vitro developed maximal apoptosis 48 hours after irradiation, keratinocytes were more resistant to UV induced apoptosis than the others. The withdrawal of growth factors from keratinocyte and addition of cycloheximide decreased the cell survival rate following UV irradiation and increased the induction of apoptosis. And ATA inhibited UV induced apoptosis. Conclusion: These results indicate that human keratinocytes have both anti-apoptotic factors and apoptosis inducing factors to maintain the homeostasis. And survival signals mediated through growth factors or cellular proteins are responsible for the resistance to apoptosis observed in keratinocytes in vitro.

토끼털에 의한 직업성 천식 의증

이선녕 ( Shun Nyung Lee ),이석정 ( Seok Jeong Lee ),노진규 ( Jin Kyu Noh ),이민수 ( Min Su Lee ),이남석 ( Nam Seok Lee ),이부길 ( Bu Ghil Lee ),리원연 ( Won Yeon Lee ),용석중 ( Suk Joong Yong ),신계철 ( Kye Chul Shin ),김상하 ( Sa 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.4

Occupational allergic rhinitis caused by rabbit fur has been reported in Korea. However, occupational asthma due to rabbit fur has not yet reported in Korea. We report a suspected case of allergic asthma caused by rabbit fur on occupational exposure. A 35-year-old male developed dyspnea, chest discomfort related to rabbit fur. He has been worked for a spinning factory. He presented with a history of allergic rhinitis and asthma. Peripheral blood eosinophil count was 690/mm3. Bronchodilator response was positive, the results of skin prick test were positive for house dust mite, cockroach and rabbit fur. The level of specific IgE for rabbit fur was 8.39 KU/L. The methacholine challenge test was positive (PC20 1.96 mg/mL). These findings suggest that the rabbit fur could induce occupational asthma. We could not perform bronchoprovacation test with rabbit fur due to patient`s refusal. He is in a well controlled state with the use of a combination of inhaled corticosteroid and long acting β2 agonist after the avoidance of rabbit fur. (Korean J Asthma Allergy Clin Immunol 2008;28:309-312)

The Effects of Bronchoscope Diameter on the Diagnostic Yield of Transbronchial Lung Biopsy of Peripheral Pulmonary Nodules

Lee, Nakwon,Kim, Sang-Ha,Kwon, Woocheol,Lee, Myoung Kyu,Yong, Suk Joong,Shin, Kye Chul,Jung, Ye-Ryung,Choi, Yeun Seoung,Choi, Jiwon,Choi, Ji Sun,Lee, Won Yeon The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.77 No.6

Background: Transbronchial lung biopsy (TBLB) is a valuable diagnostic tool for peripheral pulmonary lesions. The diagnostic yield of TBLB reportedly ranges from 41%-60%. Many studies demonstrated the various factors that influence the yield of TBLB, including size, location, and distance from the carina or pleura. However, no study has evaluated the effects of the bronchoscope diameter. We evaluated whether the bronchoscope diameter affected the diagnostic yield of TBLB. Methods: We reviewed records from 178 patients who underwent TBLB using bronchoscopes of two different diameters (5.7 mm, thick outer diameter, Olympus BF-200; 4.9 mm, thin, BF-260). The fluoroscopic guidance rates, yield of TBLB and flexible bronchoscopy (FB) were compared between the two groups. Additionally, we compared the results of the procedures with respect to diagnosis, distance from the pleura, and size of the lesion. Results: The results of fluoroscopic guidance, TBLB, and FB yield using thin diameter bronchoscope were significantly better than those obtained with a thick diameter bronchoscope (p=0.021, p=0.036, and p=0.010, respectively). Particularly, when the distance from the pleura was ${\leq}10mm$, success rates for fluoroscopic guidance and FB with thin bronchoscope were higher (p=0.013 and p=0.033, respectively), as compared to with thick bronchoscope. Conclusion: A thinner diameter bronchoscope increased the yield of bronchoscopy, and bronchial washing in conjunction with TBLB was useful in the diagnosis of peripheral pulmonary nodules.

The Efficacy of Immediate Diet for Reducing Local Adverse Events of Inhaled Corticosteroid: A Pilot Study

Lee, Myoung Kyu,Lee, Won Yeon,Yong, Suk Joong,Shin, Kye Chul,Kim, Chong Whan,Lee, Ji-Ho,Jung, Saehyun,Jung, Ye-Ryung,Kim, Hyun Sik,Yu, Tae-Sun,Kim, Sang-Ha The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.73 No.2

Background: Local adverse events associated with inhaled corticosteroid use, including dysphonia, pharyngitis and oral candidiasis, can affect adherence for treatment. 'Mouth rinsing method' has been used for reducing local adverse events, but it cannot ensure complete prevention. The goal of this pilot study was to identify whether the 'immediate diet method' can reduce local adverse events in a limited number of patients. Methods: The study was conducted in a total of 98 patients, who had been prescribed a medium-dose fluticasone propionate for the first time, from January to October in 2010. One training nurse had performed the education on how to use the inhaler, including the mouth rinsing method. And with follow-ups at one month intervals, any patient who experienced such adverse events were educated on the immediate diet method, having a meal within 5 minutes after using an inhaler and they were checked on any incurrence of adverse events with one month intervals for 2 months. Results: The mean age of patients was 65.9 years old. The local adverse events had incurred from 18.4% of the study subjects. When performed the follow-up observation in 18 patients with local adverse events after education on the immediate diet method, 14 patients (77.8%) had shown symptomatic improvements. Three of 4 patients did not show any improvement, in spite of implementing the immediate diet method. The other 1 patient did not practice the immediate diet method properly. Conclusion: The immediate diet method may be useful in reducing the local adverse events, caused by the use of inhaled corticosteroid.

위 ( 胃 ) 에 발생 ( 發生 ) 한 혈관외피세포종 ( 血管外皮細胞腫 ) ( 例 )

이계희(Kye Heui Lee),박경주(Kyung Joo Park),김동린(Dong Chan Kim),이수영(Su Young Lee),경춘숙(Choon suk Kyoung),최상전(Sang Jeon Choi),손인(In Sohn),박성훈(Seong Hoon Park),전호경(Ho Kyung Chun),최신은(Shin Eun Choi) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3

Hemangiopericytoma of stomach is an extremely rare tumor, Recentely we experienced a case of gastric. hemangiopericytoma of a 51-year-old man with massive upper gastrointestinal bleeding, The rarity of thie case is emphasized and the literature reviewed, The hemangiopericytoea of stomach is characteril by mild epigaetric pain, slowly growing mass and upper gaetrointestinl bleeding, In the limited exrience reported so far, partial gastric resection may be an effective form of treitment.

여대생들의 화장지식 및 화장행태에 관한 연구

이계숙(Lee Kye-Suk),최순덕(Choi Soon-Duk),나영순(Na Young-Soon) 한국인체예술학회 2004 한국인체미용예술학회지 Vol.5 No.1

This study was conducted to help women to increase activeness and self-confidence in social life with healthy and beautiful skin by helping them possess correct definition and accurate understanding about the make-up by understanding the relationships among the motivation, belief, value and the forms of the make-ups on the object of freshmen female university students in Chenbuk region and to provide basic data of educational program development. As a result of conducting this research, female college students regard make-up as a prerequisite in expressing their artistic senses through the make-up, and wanted to possess activeness and self-confidence. In relevance to the forms and methods of wearing make-ups, a formation of correct living habits must be actively considered, and effective information about skin, knowledge about wearing make-ups, correct utilization method of the cosmetics, symptoms and treatments about the side effects must be provided to the students along with many opportunities for the students to learn systematic way of wearing make-ups.

우리나라 원발성 간세포암 조직에서 중합효소 연쇄반응을 이용한 B 형 간염 바이러스 DNA 의 검출

이계희(Kye Heui Lee),고광철(Kwang Cheol Koh),이효석(Hyo Suk Lee),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한내과학회 1994 대한내과학회지 Vol.46 No.3

N/A Objectives : The advances in molecular biological technique enable us to detect the integrated HBV DNA in the chromosomal DNA of hepatocellular carcinoma (HCC) cells by Southern blot analysis. The recent development of the polymerase chain reaction made possible the detection of single-copy HBV DNA in a hepatocyte. Regarding the oncogenesis of HBV-related HCC, X-gene is highly suggested to be oncogenic in a transgenic mice model. We investigate the detection rate of each HBV subgenome in the HCC tissues to approach to the human hepatocarcinogenesis using the most sensitive polymerase chain reaction (PCR). Methods: Thirteen formalin-fixed paraffin-embedded HCC-tissue blocks which were aseptically obtained by surgical resection in Korea were studied; 10 were positive for serum HBsAg and one was positive for serum anti-HBc alone, and two were negative for all HBV serological markers. Cellular DNA was extracted from a deparaffinized slice of each tissue block, and then each subgenomic HBV DNA was amplified by PCR using a pair of primers corresponding to each subgenome. The amplified HBV DNA subgenomic fragment was detected by Southern blot hybridization. Results : Among 10 HBsAg-positive cases, the s-gene, c-gene and x-gene nucleotide sequences were detected in 5(50%), 4(40%) and 9(90%) cases, respectively, All 3 gene sequences were detectable in 4(40%) cases. However, HBV DNA subgenomic fragment was not found in the anti-HBc-only case and the cases without any HBV serological marker. Conclusion: It was concluded that HBV may not be involved at all in the development of HBsAg-negative HCC while HBV, especially x-gene, played a major etiologic role in HBsAg-positive HCC in human hepatocarcinogenesis.