The effects of codon usage on the formation of secondary structures of nucleocapsid protein of peste des petits ruminants virus

Xiao‑xia Ma,Yi‑ning Wang,Xiao‑an Cao,Xue‑rui Li,Yong‑sheng Liu,Jian‑hua Zhou,Xue‑peng Cai 한국유전학회 2018 Genes & Genomics Vol.40 No.9

The nucleocapsid (N) protein of peste des petits ruminants virus (PPRV) with a conserved amino acid usage pattern plays an important role in viral replication. The primary objective of this study was to estimate roles of synonymous codon usages of PPRV N gene and tRNA abundances of host in the formation of secondary structure of N protein. The potential effects of synonymous codon usages of N gene and tRNA abundances of host on shaping different folding units (α-helix, β-strand and the coil) in N protein were estimated, based on the information about the modeling secondary structure of PPRV N protein. The synonymous codon usage bias was found in different folding units in PPRV N protein. To better understand the role of translation speed caused by variant tRNA abundances in shaping the specific folding unit in N protein, we modeled the changing trends of tRNA abundance at the transition boundaries from one folding unit to another folding unit (β-strand → coil, coil → β-strand, α-helix → coil, coil → α-helix). The obvious fluctuations of tRNA abundance were identified at the two transition boundaries (β-strand → coil and coil → β-strand) in PPRV N protein. Our findings suggested that viral synonymous codon usage bias and cellular tRNA abundance variation might have potential effects on the formation of secondary structure of PPRV N protein.

Entanglement Dynamics of Multipartite States under an XY Quantum-Critical Environment

Xiao San Ma,An Min Wang 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6

By using negativity as an entanglement measure, we investigate the entanglement dynamics of multipartite quantum states under an XY quantum environment, which can exhibit a quantum phase transition. From the results, we find that the time evolution of multipartite quantum states depends not only on the size of the system and the quantum states of concern but also on the degrees of freedom of the environment, the anisotropy, and the transverse magnetic field of the environment. Especially, we find that the entanglement decay will be enhanced by the quantum phase transition of the environment under weak coupling. By using negativity as an entanglement measure, we investigate the entanglement dynamics of multipartite quantum states under an XY quantum environment, which can exhibit a quantum phase transition. From the results, we find that the time evolution of multipartite quantum states depends not only on the size of the system and the quantum states of concern but also on the degrees of freedom of the environment, the anisotropy, and the transverse magnetic field of the environment. Especially, we find that the entanglement decay will be enhanced by the quantum phase transition of the environment under weak coupling.

Expression and Characterization of Protein Latcripin-3, an Antioxidant and Antitumor Molecule from Lentinula edodes C91-3

Ann, Xiao-Hua,Lun, Yong-Zhi,Zhang, Wei,Liu, Ben,Li, Xing-Yun,Zhong, Min-Tao,Wang, Xiao-Li,Cao, Jing,Ning, An-Hong,Huang, Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

In this study, an anti-oxidant and anti-tumor protein Latcripin-3 of Lentinula edodes C91-3 was expressed in Escherichia coli. for the first time. According to the cDNA library, the full-length gene of Latcripin-3 was cloned by the methods of 3'-full rapid amplification of cDNA Ends (RACE) and 5'-full RACE. The structural domain gene of Latcripin-3 was inserted into the pET32 a(+). The functional protein of Latcripin-3 was expressed in Rosetta-gami (DE3) E. coli, evaluated by Western blotting and mass spectrometry. DPPH testing showed that the protein Latcripin-3 can scavenge free radicals remarkably well. The activity of functional protein Latcripin-3 on A549 cells was studied with flow cytometry and the MTT method. The MTT assay results showed that there was a decreases in cell viability in a dose-dependent and time-dependent manner in protein Latcripin-3 treated groups. Flow cytometry demonstrated that Latcripin-3 can induce apoptosis and block S phase dramatically in human A549 lung cancer cells as compared to the control group. At the same time, the cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. This research offers new insights and advantages for identifying anti-oxidant and anti-tumor proteins.

Postmenopausal Hormone Therapy is Associated with in Situ Breast Cancer Risk

Ni, Xiao-Jian,Xia, Tian-Song,Zhao, Ying-Chun,Ma, Jing-Jing,Zhao, Jie,Liu, Xiao-An,Ding, Qiang,Zha, Xiao-Ming,Wang, Shui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: The relationship between postmenopausal hormone therapy (HT) and invasive breast cancer has been extensively investigated, but that with breast carcinoma in situ (BCIS) has received relatively little attention. The aim of our present study was to review and summarize the evidence provided by longitudinal studies on the association between postmenopausal HT use and BCIS risk. Methods: A comprehensive literature search for articles published up to May 2012 was performed. Prior to performing a meta-analysis, the studies were evaluated for publication bias and heterogeneity. Relative risk (RR) or odds ratio (OR) values were calculated using 14 reports (8 case-control studies and 6 cohort studies), published between 1986 and 2012. Results: There was evidence of an association between ever postmenopausal estrogen use and BCIS based on a random-effects model (RR = 1.25, 95% confidence interval (CI) = 1.01, 1.55). However, we found no strong evidence of an association between ever postmenopausal estrogen combined with progesterone use and BCIS using a randomeffects model (RR = 1.55, 95% CI = 0.95, 2.51). Furthermore, our analysis showed a strong association between "> 5 years duration" of estrogen or estrogen combined with progesterone use and BCIS. Furthermore, current use of any HT is associated with increased risk of BCIS in cohort studies. Additional well-designed large studies are now required to validate this association in different populations.

Yesod를 이용한 Haskell 웹 애플리케이션 개발 방법에 대한 실험

안형준(Hyungjun An),류샤오(Xiao Liu),변석우(Sugwoo Byun),우균(Gyun Woo) 한국정보과학회 2016 한국정보과학회 학술발표논문집 Vol.2016 No.6

Synthesis of coryphantha elephantidens-like SnO2 nanospheres and their gas sensing properties

Xiao-xue Lian,Yan Li,Junwu Zhu,Yun-ling Zou,Xiulin Liu,Dongmin An,Qiong Wang 한국물리학회 2019 Current Applied Physics Vol.19 No.7

In this work, coryphantha elephantidens-like SnO2 with porous structures were prepared successfully by a simple hydrothermal route, through adjusting the temperature of hydrotherm. Its morphology was characterized by Xray diffraction (XRD), scanning electron microscopy (SEM) and Brunauer–Emmett–Teller (BET). Compared to the regular nanospheres, the coryphantha elephantidens-like SnO2 nanospheres had obviously higher gas-sensing response, owing to the special structure with large specific surface area (161.16m2 g−1). It surprised us that the coryphantha elephantidens-like SnO2 sensor could easily distinguish between ethanol and acetone, whose chemical property were similar. Moreover, it also exhibited wide measurement range, fast response speed (less than 10 s) and good repeatability at a low temperature (180 °C) to ethanol. The desirable specific surface area and pore volume were conducive to molecules adsorption and diffusion, which were believed to be the major cause of the improvement of gas sensing performance.

Synthesis and Characteristic of Novel Soluble Triazoleimide Oligomers with Terminated Arylacetylene

Xiao’an Zhou,Lei Du,Liqiang Wan,Xiaofei Wang,Yanpeng E,Farong Huang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

Novel soluble triazoleimide oligomers terminated with arylacetylene terminated were synthesized by the Cu(I)-catalysed 1,3-dipolar cycloaddition polymerization of diazides and imide-containing dialkyne. Several molecular weight triazoleimide oligomers were prepared from diazide and dialkyne monomers with different stoichiometric combinations. The curing behaviors of the oligomers were tested by differential scanning calorimetry (DSC). The thermal properties of the cured products were evaluated by DSC and thermogravimetric analysis (TGA). These cured oligomers showed the glass transition temperature of about 225 - 235 oC and the decomposition temperature (at 5% weight loss) of about 385 - 393 oC in nitrogen.

NATURE AND CATALYTIC PERFORMANCE OF PtSAPO-5 MOLECULAR SIEVE CATALYST SYNTHESIZED BY ONE STEP

Xiao, Tian cun,Wang, Hai tao,Lu, Yu li,An, Li dun,Wang, Hong li 한국화학공학회 1998 Korean Journal of Chemical Engineering Vol.15 No.5

Pt catalysts supported on silicoaluminophosphate molecular sieves were synthesized by adding platinum salts directly to the gel mixture of SAPO. The synthesized products were marked as PtSAPO-5, in order to distinguish with the SAPO-5 supported platinum catalyst prepared by impregnation, which was marked as Pt/SAPO-5. The as-synthesized and calcined PtSAPO-5 samples were characterized by XRD, ^(29)Si MAS NMR, ^(129)Xe NMR and NH₃-TPD, and their catalytic activities were evaluated by the, hydroisomerization of n-hexane. The results revealed that in PtSAPO-5, the platinum could catalyze the removal of the templates entrapped in the as-synthesized samples. The presence of Pt in the gel mixture can increase silicon content in the framework of SAPO-5, and the acid sites. In the activated PtSAPO-5 catalyst, Pt was highly dispersed in the channel of SAPO-5 molecular sieve. Pt exists in the form of big particles outside of the channel of Pt/SAPO-5 prepared by impregnating method, and impregnation with H₂PtCl_6 improved the strength of acid sites. PtSAPO-5 has a higher activity and selectivity than Pk/SAPO-5 in the hydroisomerization of n-hexane. This is because the former has medium acid sites and high dispersion of Pt, while the channel of Pt/SAPO-5 is somewhat retarded by the big platinum particles, and the acid sites are stronger than the former.

Changes in Median Ages at Death from Selected Cancer Types in Relation to HLA-DRB1/DQB1

An, Wan-Xin,Fan, Ya-Xin,Liang, Xiao-Hua,Liu, Hui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

The median ages at death from cancers between 1985 and 2005 were calculated to demonstrate that inherent anticancer mechanisms may be a common pathway for different cancers. Seventy-eight patients with gastric, liver and lung cancers, were recruited in the solid cancer group. The leukemia group consisted of 31 patients with three main types of leukemia. The controls were 100 healthy individuals. The samples were typed using an HLA-DR/DQ PCR-SSP typing kit. The results showed that the median ages at death from all causes were 64.7 years in 1985 and 70.1 years in 2005. The range of the median ages at death from all cancers was similar to the corresponding value for deaths attributed to all causes. The frequency of $DRB1^*03$ was 9.6% in the solid cancer group and 3.0% in the control group (p<0.05). The frequency of $DRB1^*04$ in the leukemia group were significantly lower than that of the control (p<0.05). $DRB1^*13$ and $DRB1^*06$ frequencies in the leukemia group were significantly higher than those of the controls (p<0.05). It is suggested that inherent anti-cancer mechanisms may be a common pathway for different cancers and are associated with the immune system and HLA.

Prognostic Significance of Preoperative Serum Alpha-fetoprotein in Hepatocellular Carcinoma and Correlation with Clinicopathological Factors: a Single-center Experience from China

An, Song-Lin,Xiao, Ting,Wang, Li-Ming,Rong, Wei-Qi,Wu, Fan,Feng, Li,Liu, Fa-Qiang,Tian, Fei,Wu, Jian-Xiong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Objectives: To investigate the prognosis significance of preoperative serum alpha-fetoprotein (AFP) and the correlation with clinicopathological factors of hepatocellular carcinoma (HCC) patients who underwent hepatectomy. Materials and Methods: Clinicopathological data of retrospective analysis were collected for 251 HCC patients undergoing hepatectomy in this study. According to preoperative AFP level, patients were categorized into AFP-negative (0-20ng/mL) and AFP-positive (>20 ng/mL) groups for Kaplan-Meier analysis and Cox proportional hazard regression modeling. Results: The results demonstrated that increased AFP was associated with longer prothrombin time (PTs), liver capsule invasion, low grade differentiation, and late Barcelona Clinic Liver Center (BCLC) stage. Moreover, the female patients had a greater prevalence of increased preoperative AFP than male patients [284.8 (3.975-3167.5) vs (3.653-140.65); Z-2.895, p=0.004]. The 1-, 3-, and 5-year recurrence-free survival (RFS) rates were 78.1, 57.5, and 40.6 % in the AFP-negative group and 61.8, 37.7, and 31.4 %, respectively, in the AFP-positive group (log-rank test 8.312, p=0.004). The 1-, 3-, and 5-year overall survival (OS) rates were 94.4, 83.8, and 62.3% in the AFP-negative group and 87.2, 60.0, and 36.7%, respectively, in the AFP-positive group. The difference was statistically significant (log-rank test, 16.884, p=0.000). Cox proportional-hazards model identified preoperative AFP to be an independent prognostic predictor of overall survival. Conclusions: Preoperative serum AFP is an independent predictor of prognosis among HCC patients following surgical resection. Female patients have a higher preoperative AFP than their male counterparts.