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- 학과정보 : 농학과 전공 (검색결과 1,909 건)
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機械移秧 散播箱子育苗時 播種量과 植傷處理가 苗素質 및 初期生育에 미치는 影響
水稻 機械移秧 散播箱子를 이용한 育苗時 建苗育成方法을 모색하기 위하여 기존의 箱子當 130g 播種量보다 播種量을 줄이면서 缺株 防止를 위해 移秧機 苗取거리에 줄뿌림을 하고 苗素質과 生育反應을 검토하였다. 統一型 品種인 용문벼와 자포니카型 品種인 화성벼를 供試하여 1989年 作物試驗場 沓作圃場에서 試驗을 遂行한 結果를 要約하면 다음과 같다. 1. 苗의 草長, 葉數, 乾物重 및 乾物/草長比 등 苗素質은 密播인 箱子當 130g播種量보다는 40g으로 播種量이 적을수록 增加하였다. 2. 苗體內 全炭水化物 및 全窒素含量, C/N率은 箱子當 130 ~ 55g 播種量間 차이가 크지 않았으며 燐酸, 加里, 마그네슘 및 규산함량은 密播苗에서 높았다. 3. 아미노산含量은 箱子當 130g 播種量에 비해 箱子當 40g ~ 55g 播種量에서 크게 增加하였다. 4. 移秧時 缺株率은 播種量을 줄이면서 줄뿌림한 育苗에서도 散播 130g 育苗와 큰 차이가 없어서 벼 栽培면에서 지장이 없을 것으로 생각되었다. 5. 出穗期는 箱子當 播種量 40~55g 處理가 散播 130g처리보다 5~8일 빨랐는데 이는 移秧 당시의 葉數差異에 따른 것으로 생각되었다. 6. 苗에 대한 剪葉 및 斷根處理時 잎보다는 뿌리 절제시 生育抑制 效果가 큰 경향이었으며 뿌리의 切斷程度가 클수록 地上 및 地下部 生育이 抑制되었다. 地上部는 上位 2葉보다 上位 1葉 절제시 生育抑制 정도가 컸다. 7. 散播 130g 播種苗에 비해 40g 播種하고 줄뿌림한 苗는 剪葉 및 斷根處理時 특히 地上部 建物中, 根數 및 發根力은 뚜렷하게 增加하였으며 品種間에는 용문벼(統一型) 보다는 화성벼(자포니카型)에서 뚜렷하였다. 8. 機械移秧 散播箱子에 130g 播種하는 것보다 播種量은 55 ~ 70g으로 줄이면서 缺株防止를 위해 줄뿌림 하면 健苗育成 및 增收效果가 뚜렷함이 究明되었다. This study was carried out to investigate the raising method of rice healthy scedings for machine transplanting cultivation by reducing seeding rate of 130g per broadcast-sowed box and decreasing missing hill percent. Seeding rats less than 130g per box with sowing method of 14,16 and 18 rows seeding for reducing missing hill were treated with two rice cultivars: Yongmoonbyeo(Tongil type, ludica x Japonica) and Hwaseongbyeo(Japonica type)at crop experiment station RDA in 1989. The results obtained were Summarized as follows: l. Seedling height, number of leaves, shoot dry weight and shoot dry weight/ seedling height ratio was increased in 40g row seeding per box than 130g broadcasting. 2. Totol corbohydrate and nitrogen, C/N ratio were not significantly different among seeding rates betwcen 130 ~ 55g/box, but contact of phosphate, potassium, magnesium and silica were higher in dense seeding. 3. Amino acid contents was increased greatly in seeding rate of 40~55g per box than that of 130g broadcast. 4. The missing hill ratio of reduced seeding rate to 40~55g with row seeding in seedling box was not significantly increased compared to that of 130g broadcast sowing. 5. Heading dates were 5~8 days faster in seedling of 40~55g row seeding per tray than in that of 130g broadcasting mainly due to the progress of seedling ieaf age at transplanting time. 6. The growth of root damaged seedlings resulted in more retard of shoot and root growth than shoot damaged seedling at 15 days after transplanting. 7. Rice seedling of 40g per tray with row seeding resulted in significant increase of shoot dry weight, number of roots and rooting ability than 130g broadcast under defoliation and /or root cutting treatments, especially in cultivars yongmoonbyeo(Tongil type) than Hwaseongbyeo(Japonic type) 8. It was concluded that the of reduced seeding rate of 40~55g per tray row seeding brought about healthy seedling particularly in transplanting of damaged seedling than broadcasting of 130g per tray.
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호르몬 농도 차이에 따른 메밀의 부정배형성과 식물체 재분화
Buckwheat is an important economic plant, as a food plant, due to high protein value in the seeds. Buckwheat has for centuries remained a crop with low seed set due to certain characteristics which prevent the application of conventional breeding methods. Currently, information about tissue culture in buckwheat is limited and restricted to micropropagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl as explant of the cultivated buckwheat species Fagopyrum esulentum, differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-D e.0 mg/L), and BAP 1.0 mg/L), and 3% sucrose. Friable callus was transferred solidified MS media containing BAP 1.0 mg/L), and at various concentrations for the induction of somatic embryogenesis. The optimum concentrations of additves were IAA (2.0 mg/L), KIN (1.0 mg/L), BAP (1.0 mg/L) and 3% (w/v) sucrose. Only 2,4-D did not show and significant effect on callus induction or embrygenesis. showed the significant effect in hypocotyls of common buckwheat. Regenerated plants after acclimation will transfer to green house. The seeds on regenerated plants in matured within 100 days. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species Fagopyrum tataricum, differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-D (2.5 mg/L) and BAP (1.5 mg/L) and 3% sucrose. Friable callus was transferred to solidified MS media containing BAP (1.0 mg/L) and at various concentrations for the induction of somatic embryogenesis. MS medium containing 2,4-D (2.0 mg/L), 2-iP(10 mg/L), and KIN (0.2 mg/L) also showed highest callus induction under light condition. Regeneration of embryonic callus varied 5% to 20%. Whole plants were obtained at high frequencies when the embryogenic calluses with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. For plant regeneration, MS medium containing IAA (0.1 mg/L), KIN (1.0 mg/L), BAP (2.0 mg/L), and TDZ (0.5 mg/L) showed the significant effect in hypocotyls of tatary buckwheat. The main objective of this research was to develop a efficient protocol for plant regeneration for common buckwheat, and tatary buckwheat to apply in future for genetic transformation, and also the mechanism of expression of totipotency in plant cells.
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