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- 저자 : LOWE BABOUCARR (검색결과 1 건)
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LOWE BABOUCARR 부경대학교 과학기술융합전문대학원 2016 국내석사
Tissue engineering processes involve the design and development of functional biomaterial substitutes which can serve as carbon copy of the extracellular matrix and produce the essential information for cells to capacitate tissue development processes. Bone tissue engineering provides us with the option to repair damage bone tissues emanating from injuries sustained as a result of bone fracture, osteoarthritis, tumor, trauma and other congenial diseases. As a result of growing demand for bone grafts, research in the area of artificial organ development using functional biomaterials as suitable prostheses in reconstructive surgery for the treatment of loss or damage tissue or organs is gaining momentum. In this respect, Nano-Hydroxyapatite (nHA) was isolated from salmon bone via an alkaline hydrolysis. The resulting nHA was characterized using several analytical tools, including thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous in nature. Microscopic examination further revealed that the isolated nHA possessed a nanostructure with a size range of 6–37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. Further, a solid three dimensional (3D) composite scaffold with a high potential usage for bone tissue engineering was prepared using freeze drying method composed of chitosan (C), natural nano-hydroxyapatite (nHA) isolated from salmon bones and fucoidan (F), (C-nHA-F). Fourier transform infrared spectroscopy (FT-IR), Thermal Gravimetric Analysis (TGA), X-ray Diffraction Analysis (XRD), Scanning Electron Microscopy (SEM) and Optical Microscopy (OM) were used to determine physiochemical constituents and morphology of the scaffold. The addition of nHA in the C-F composite scaffold reduce the water uptake and water retention ability. FT-IR analysis shows the presence of carbonated group in the scaffold which is due the presence of nHA that was isolated via alkaline hydrolysis from salmon fish bones. Microscopic results indicated that dispersion of nHA and fucoidan in the chitosan matrix were uniform and showed the pore size (10 to 400 µm) of the composite revealing a suitable micro architecture for cell growth and nutrient supplementation. This was further elucidated in vitro using Periosteum-derived Mesenchymal Stem cells (PMSCs) and revealed profound biocompatibility and excellent mineralization. Thus, we suggest that with further in vivo and clinical investigations, Chitosan-nHA-Fucoidan nanocomposite will be a promising biomaterial for use in bone tissue regeneration applications.
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