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      • Effects of cytokines on cell viability in Porphyromonas gingivalis-infected KB cells

        최용진 Chonnam National Univ. 2007 국내박사

        RANK : 247631

        치주질환에 있어서 Porphyromonas gingivalis는 질환을 일으키는 매우 중요한 요소이다. 이 연구의 목적은 P. gingivalis에 감염된 구강상피세포의 손상에 대한 cytokine의 효과를 살피는 것이다. 이번 연구에서는 P. gingivalis 초기감염에서 cytokine의 효과를 살펴보기 위해 적은 수의 P. gingivalis (MOI=10)가 사용되었으며, cytokine (IL-1β, TNF-α, INF-γ) 단독처리, P. gingivalis 단독처리, cytokine과 P. gingivalis 병용처리에 따른 세포손상, 세포주기, ROS (H₂O₂, O₂^(·-)) 생성과 NADPH oxidase mRNA의 발현의 변화를 평가하였다. KB세포에 cytokine (IL-1β, TNF-α, INF-γ) 단독처리와 P. gingivalis (MOI=10) 단독처리시에는 세포손상이 미미하였으나 병용처리시에는 12시간부터 유의한 세포손상이 관찰되며 (≥30%), 세포의 손상 심화정도는 P. gingivalis와 TNF-α, P. gingivalis와 INF-γ, P. gingivalis와 IL-1β 병용처리 순서였다. 세포주기 변화는 cytokine 단독처리시 G0/G1기의 세포수 증가 경향을 보였고, cytokine과 P. gingivalis 병용처리시에는 S기의 세포수 증가 경향이 관찰되었다. 또한 KB세포의 ROS 생성은 병용처리시에 단독처리군보다 더 많은 H₂O₂ 생성을 보였으나 O₂^(·-) 생성은 모든 경우에서 변화가 관찰되지 않았다. NADPH oxidase mRNA 발현의 변화도 cytokine 단독처리시 NOX4와 Rac2가 24시간에 증가하였고, 병용처리시에는 24시간에 Rac2, p22^(phox)는 증가하였으나 NOX4와 NOX2는 감소하는 경향을 나타내었다. 이상의 결과를 요약하면 P. gingivalis에 감염된 KB 세포에 cytokine 처리는 세포손상을 현저히 심화시켰으며 세포손상기전에는 H₂O₂와 같은 활성산소종의 생성 증가에 기인할 수 있음을 시사하였다. 따라서, P. gingivalis에 감염시 cytokine의 존재가 치주질환의 형성과 진행과정y에 중요한 역할을 하리라 사료된다. The Porphyromonas gingivalis has been implicated as an important bacterial species in pathogenesis of periodontitis. The purpose of this study was to investigate the effects of cytokines on cell viability in P. gingivalis-infected KB cells. In this study, low numbers of P. gingivalis (MOI=10) was used to see the effects of cytokines in the early phase of P. gingivalis infection. The cell viability, cell cycle, intracellular reactive oxygen species (ROS) generation, and NADPH oxidase mRNA expression level were assessed in response to treatment of cytokine (IL-1β, TNF-α, or INF-γ), treatment of P. gingivalis, or combination treatment of cytokine and P. gingivalis. When KB cells treated with cytokine or P. gingivalis alone, cell viability was slightly decreased (□10%). On the other hand, when KB cells were treated with combination of cytokine and P. gingivalis, cell viability was profoundly decreased at 12hr incubation (≥30%). The degree of severity in cell death was in the order of ①P. gingivalis - IL-1β, ②P. gingivalis - INF-γ, ③P. gingivalis - TNF-α combination treatment. The results of cell cycle analysis by flow cytometry showed the inhibition of cell growth at G0/G1-phase in cytokine-treated groups and S-phase in combination-treated groups. Intracellular ROS production of KB cells showed higher levels of H₂O₂ in combination-treated groups than in cytokine-treated or P. gingivalis-treated groups. However, the levels of superoxide anion was not changed in any condition. The expression level of NADPH oxidase mRNA showed increased NOX4 and Rac2 expression at 24 hr in cytokine-treated group. In combination-treated groups, increased expression of Rac2 and p22^(phox) and decreased expression of NOX4 and NOX2 were observed at 24hr. In summary, the cytokine treatment resulted in severe cell death in P. gingivalis-infected KB cells. And increased H₂O₂ production might be involved in cell death. Therefore, cytokine seems to play an important role in early phase of P. gingivalis infection of periodontitis.

      • 인지 시스템 설계과정에 적합한 시스템 엔지니어링 프로세스 모델의 연구

        최용진 금오공과대학교 2013 국내석사

        RANK : 247631

        The aim of this study is to propose the design process suitable for developing the cognitive interface considering system engineering process (SEP) models. Due to the cognitive workload in an operation of HMS, some cognitive interfaces have been developed. It is somehow difficult to use the developed cognitive interface in real working environment since they often showed a conflict to stereotyped interface. So it is necessary to develop the design process suitable for the more operator-specific interface. Various SEP models were reviewed for selecting the suitable design process which might resolve the problem from design-specific interface. The suitable process for designing cognitive interface was proposed considering currently usable SEP models. The findings from the study may be helpful for systematic approach to designing cognitive interface in digitalized environment. The proposed design process would be applied for easily employing the cognitive interface in digitalized working environment such as main control room in nuclear power plant.

      • 왕커핑 목조의 조형적 특성에 관한 연구

        최용진 圓光大學校 2013 국내박사

        RANK : 247631

        Wang Keping, a wood carver, was born at a small town of Hebei Sheng around Beijing with an artistic gift from his parents in 1949. But he had to waste his high school days in a period of Great Cultural Revolution. Then he endeavored to be a writer or film star that he had wanted to be, but he suffered from pang not to select such a job by dint of his rebellious character against Communist system owing to Great Cultural Revolution. As an alternative, he began to paint according to a suggestion by a painter friend, with which he could not be also satisfied, and finally determined to be a sculptor. But whereas painting has a long history in China, sculpture does not. Thus what he sculpts for himself is rather a rational truth more or less. For other sculptors at that time had to trod a road of self-education like him. Theme of his initial sculptures was of rebellion or ridicule against political system without exemption. That is, in the initial sculptures such as Silence, Chain, Idol, Fanatic, etc., he expressed the attack towards Mao Zedong or Chinese people's appearance suffering from Communist regime metaphorically. By the way, it ostensibly seemed to give the artists freedom of creation since Great Cultural Revolution, but virtually rather strengthened control and surveillance. Artists revolted against such an artistic, political establishment of Chinese government. It was a crisis of Tiananmun. The group that guided them in this protest was very ‘Xing Xing Art Group’. In a demonstration by that group members that compared themselves to an endlessly glittering star, Wang Keping was one of leading persons. They were extremely regulated by the government in the freedom of creation. With three group exhibitions, they not only exploited a road to their own autonomous growth, but guided to a road enough to create and display avant-garde art to young artists. Since then, there was a wave of liberation for a moment, but the government's suppression was more extreme. As a result, some artists and intellectuals was sent to prison, and he searched for a political asylum with international marriage along with other artists. After getting married with a French lady, and defected to France, Wang Keping's art world reached a turning point. That is a very transition from the representation of political satire to human body, in particular, to that of pure beauty in female body. And after his defection he concentrated much concern on a medium of tree. In fact, he had showed much concern on tree from the beginning of wood carving in China, it was hard to get timber for carving owing to deforestation since the foundation of China. But the abundant timbers in France served for blooming his artistic inspiration. He began to regard tree as organic body. Thus he did not treat carelessly any of trunk, twig, growth ring, knot, etc. that each tree has. He awakened that they harmonize one another in order to create a tree, and a beautiful woman's body that he wants to represent is formed by their combination. That is, he, like a Neo-platonist, believed that an idea of a beautiful woman is incarnated through a material of tree. It was a reason that he implants woman's body and tree with life, energy, germination and growth simultaneously. Wang Keping's philosophy of art started with philanthropy. With an eyewitness that Chinese leaders exerted suppression and surveillance over people in a period of Great Cultural Revolution, his philosophy of philanthropy was germinated. It was a result of far-reaching love towards China. He searched for love towards China from not only such love towards human-being, but also that towards tradition of culture and art. But that love was not wide-spread nor comprehensive, rather it was only selected in accordance with his artistic idea. What he preferred to Taoism or Zen Buddhism was not to believe them, but to incite only parts harmonized with his own among idea or spirit contained within them. And it was in this context that he enumerated the sculptures in Han Dynasty or the art of Qui Baishi. Additionally, the reason that he accepted philosophy or technique of Brancusi or Zadkine that he found from the history of Western art was in that they preferred to represent the essence of life and on the contrary omitted the trivial things. In conclusion, the plastic language displayed in Wang Keping's wood sculpture is typically of Taoistic character, which discloses his Taoistic philosophy of nature and world, and belongs to a comprehensive concept containing the philanthropic. That is, in that he discusses and represents the essential relationship between his own free, quiet life and art, female body and tree, the world of his sculpture is called Taoistic, Zen Buddhistic. 목조 조각가인 왕커핑은 1949년 베이징 인근 허베이성 시골에서 부모의 예술가적 기질을 물려받고 태어났다. 그러나 그는 문화대혁명을 거치면서 고등학생 시절을 허비하게 되었다. 그 이후 그는 그 자신이 원했던 극본 작가나 영화배우가 되려 했으나 문화대혁명에서 기인된 공산 체제에 대한 반항아적 기질로 인하여 그 직장을 거절당하는 아픔을 겪었다. 그는 대안으로 화가 친구의 권유로 붓을 들었으나 그것도 만족스럽지 못하여 고민 끝에 조각가의 길로 들어섰다. 그러나 중국에서는 회화가 유구한 역사와 전통을 가지고 있는 반면에, 조각은 그렇지 못했다. 따라서 그가 독학으로 조각을 전공한다는 것은 오히려 당연한 이치인지도 모른다. 왜냐하면 당시의 다른 조각가들도 그처럼 독학의 길을 걸어야만 했기 때문이다. 그가 최초로 제작한 조각들의 주제는 어김없이 정치 체제에 대한 반발, 조롱이었다. 말하자면 <침묵>, <구속>, <우상>, <광신자> 등과 같은 초기작에서 그는 마오쩌둥을 비난하거나 공산 정권 아래서 신음하는 국민의 모습을 비유적으로 표현했다. 그런데 문화대혁명 이후에 예술가들에게 겉으로는 창작의 자유를 주는 듯하더니 실제로는 오히려 통제와 감시를 강화시켜 나갔다. 예술가들은 이러한 중국 당국의 예술적, 정치적 기득권층에 반기를 들었다. 그것이 톈안먼 사태였다. 이 시위에서 그들을 선도했던 그룹은 바로 ‘싱싱화회’였다. 스스로 밤에 끝없는 빛나는 별에 비유했던 ‘싱싱화회’ 회원들의 시위행진에서 왕커핑은 주동자 중 한 사람이었다. 그들은 예술 창작의 자유에서 엄격하게 통제받았다. 하지만 3회에 걸친 그룹전을 가지면서 그들은 스스로의 자발적인 성장의 길을 개척했을 뿐만 아니라 젊은 예술가들에게도 아방가르드 미술을 창작하고 전시할 수 있는 길을 열어주기도 했다. 그 이후로는 잠시 동안 자유화 물결이 일더니 당국이 탄압이 더욱 거세졌다. 그 결과 일부 예술가들과 지식인들은 투옥되었으며, 그도 일부 예술가들처럼 국제결혼으로 정치적 망명을 택하게 되었다. 왕커핑이 프랑스 여성과 결혼하고 프랑스로 망명한 이후에 그의 예술세계는 전환점을 맞게 되었다. 말하자면 정치적 풍자의 표현으로부터 인체, 특히 여성 인체의 순수한 미의 표현으로의 전환이 그것이다. 그리고 그는 망명 이후부터 나무라는 매체에 더 많은 관심을 집중시켰다. 사실상 그가 중국에서 처음으로 목조를 시작할 때부터 나무에 많은 관심을 가졌지만, 중국의 창건 이후로 남벌 등의 여파로 목조용 나무를 구하기가 어려웠다. 그러나 프랑스에서의 풍부한 목재는 그의 예술적 영감을 만개시키는데 일조하게 되었다. 그는 나무를 하나의 유기체로 간주하기 시작했다. 그래서 각각의 나무가 가지고 있는 줄기, 나이테, 매듭 등을 어느 하나도 소홀히 다루지 않았다. 그것들이 어울려 한 그루의 나무를 형성하는 것이고, 그가 그려내고자 하는 아름다운 여성 인체가 그것들의 조합으로 이루어지고 있음을 자각했다. 즉 그는 마치 신플라톤주의자처럼 아름다운 여성이라는 이념이 나무라는 물질을 통하여 구현된다고 믿었다. 그것은 그가 여성 인체와 나무에 생명, 에너지, 발아와 성장이라는 이념을 공히 이입시킨 이유였다. 왕커핑의 예술철학은 인간애에서 출발했다. 그것은 문화대혁명을 경험하면서 중국 지도층이 인민에게 가했던 탄압과 감시를 목격하면서 인간애적 철학을 싹틔운 것이었다. 그것은 지극한 중국 사랑의 결과였다. 그는 중국 사랑을 그러한 인간 사랑뿐만 아니라 문화와 예술 전통의 사랑에서도 찾았다. 그러나 그 사랑은 광범위하고 포괄적인 것이 아니라 그의 예술적 이념과 부합되는 것만을 취사선택한 것이었다. 도교나 선불교를 선호한 것도 그것들을 신봉하기 위함이 아니라 그것들에 내포된 이념이나 정신 중에서 그 자신의 것과 부합되는 것만을 인용하기 위함이었다. 그리고 그가 중국의 과거 조각이나 예술가를 존경하면서 열거한 한대의 조각이나 치바이스의 예술도 이러한 맥락에서 선택한 것이었다. 그가 서양미술사 속에서 찾아낸 브랑쿠시나 자드킨의 철학이나 기법을 수용한 이유는 그들이 삶의 본질을 형상화하고 부수적인 것은 생략하는 경향을 선호했기 때문이다. 결론적으로 왕커핑의 목조에 나타난 조형언어는 도교적인 성격이 대표적인데, 그것은 그의 도가적인 자연관, 세계관을 표방하는 것이며, 그것은 인간애적인 것을 포함하는 광범위한 개념에 속한다. 말하자면 그는 그 자신의 유유자적하는 삶과 예술, 여성 인체와 나무의 본질적인 관계성을 논하고 조형화했다는 점에서 도교적이며 선불교적이라고 말하는 것이다.

      • 순천농협 남도김치의 일본시장 수출확대 전략

        최용진 순천대학교대학원 2015 국내석사

        RANK : 247631

        Having established its cooperative association in 1972, and Kimchi facility in 1993, Suncheon Nonghyup(farmer bank)'s Namdo Kimchi is a specialized firm which have been making and distributing Kimchi for 22 years. With its accumulated capacity and advantageous environment situated in Homam grain belt and Southern coastal line around clean Suncheon region, it has produced Namdo Kimchi by reliable local and seasonal agricultural products under the brand of 「Namdo Kimchi」. By using only local agricultural products, high quality of safe and fresh products can be produced, and in salting napa cabbages, the name of a certain employee is revealed. Therefore food safety can be assured with introducing standardized salting (systemization), with securing price advantage by purchasing raw materials in bulk, and by ensuring HACCP control, production and distribution and hygienic cooling system throughout the entire production process. There are issues and prior improvements with Namdo Kimchi; First, existing buildings were acquired to increase production facility. However it rather has decreased production efficiency, and excessive investment in facilities has depreciated around KRW 400 million in a year, which are main culprit of deficits. Also facilities are getting dilapidated, they require moves to improve and modernize facilities. Second, it is considered to be urgent that transforming majority packaging facilities to small packaging from bulk packaging is considered urgent in order to expand markets and revenues. By current production methods and markets, there is a certain limit in growth, therefore it is considered difficult to turn net revenue from deficit into surplus. Third, Namdo Kimchi has weaker competitive edge regarding price due to relatively high purchasing cost of raw materials. It is because Nonghyup offers relatively higher prices for protecting members of Nonghyup Cooperative Association. Given peculiar nature of Nonghyup, there is a certain limitation in cost reduction, but they need to come up with measure to cut down cost of raw material, such as providing farmers technologies to produce products with good quality in mass. Fourth, professionals are required considering special nature of Kimchi as traditional cuisine. Therefore to produce good quality products, it is be necessary to secure and nurture meister level of workmen. Fifth, items of which sales are falling need to be streamlined and dropped out of production, and increase of human resources and specialization training for marketing are in earnest demand to aggressively strengthen marketing capacity. Furthermore, the following are regarded necessary to increase exports of Namdo Kimchi to the Japanese market; First, building the domestic and foreign networks are required for buyer negotiation. Suncheon Nonghyup needs establishment of such global network for buyer negotiation and wide range of information exchanges so to facilitate marketing for targeting Japan exports. Suncheon Nonghyup shall continue to support exports and cooperate one another through mutual cooperation agreements based on confidence. Second, Suncheon Nonghyup needs to develop demand-oriented products. According to the survey on domestic consumers regarding what products they wish to be developed, products without chemical seasoning comes first with 43.5%, followed by low-sodium products, products mostly made of organic ingredients, products with high quality supplement ingredients, and products with strengthened health-beauty contents. Meanwhile average premium which consumers are willing to pay more are revealed around 20%. On the other hand, 24.3% of overall household respond that they are willing to pay in additional from 20% up to 100%. Hence, in respond to the forementioned types of consumer preference, there is need to develop Kimchi without chemical seasoning, Kimchi with reduced sodium, organic napa cabbage Kimchi, Kimchi with high quality ingredients and Kimchi with strengthened health and beauty contents. Third, marketing is needed to create more revenues and promotion. If you look at current state of exports by Suncheon Nonghyup's Namdo Kimchi, you find that serious marketing strategy and promotion are demanded from the following figures. In 2009 its revenue stood at KRW 992 million. In 2014 the revenue plunged to KRW 314 million. In mere five years its revenue fell down to 1/3. Although they are making efforts to develop domestic demands for additional revenue, if looking at revenues on domestic regions, 57.7% for Jeollanamdo, 24% for Gyeongsangnamdo and 11.1% for Seoul, there is high dependency on Jeollanamdo and Suncheon region (43.1%). Therefore it is likely that consumers leave for other competitors particularly when there are currently ongoing changes in regard to catering school meals and bidding for catering. Also even though Namdo food have cut production costs and other expanses, its net profit and loss in short term accrued KRW 406 million in 2014, it tells that although making reliable production is important, if exports can grow revenue steadily, Namdo Kimchi can regain stable management. Therefore they need strategic marketing system through business partnership on target products, and they need to implement strategy aiming at Japan where takes account of more than 80% of their export market. Forth, they need to nurture professionalism and safety-oriented human resources. it is necessary to secure food safety control and hygienic production process and reliable distribution system in order to commercialize and sell Kimchi through the Japanese Kimchi market. To this end, as a part of efforts to export more Namdo Kimchi by Suncheon Nonghyup, it first requires to know and carry out to invite buyers for site visiting, to understand the current status, and to realize safety, specialism of food and facilities accordingly. Kimchi industry is a labour-intensive industry which calls for decent mind and professionalism of employees. And with HACCP establishment, self-audit, third party audit, daily audit and others can be constantly kept in practice so that it can develop and maintain human resources for safe Kimchi production. In addition, there is a need for training on processing, distribution and promotion and marketing which aims to innovate minds fast to catch up changes in Kimchi market of Kimchi industry, a need for changing minds to be working level-oriented, and a need for serious training for understanding on Kimchi, function of Kimchi, safety and step-wise hygiene control system. It is needed to form quality and hygiene-priority mind suitable to the voluntary and participatory HACCP system, and finding issues and suggesting how to improve them are to be normalized in daily life. In a nut shell, they need to revolution by introducing mind transformation to consumer-oriented mind away from producer-oriented mind. Fifth, facilitation and establishment of practical infrastructure linkage are required. Marketing strategy is required to focus on how to sell rather than focusing on producing for sake of Japan exports, and product cycle, preference of Japanese consumers, changes in Kimchi markets, and specialized human resources in regard to safety would be also important factors as much as establishing practical infrastructure linkages in order to ensure export competitiveness. To prepare for dwindling Korean Kimchi producers, we need to understand the distribution system in Japan through Japanese vendors who are similar to wholesale jobbers, and need to come up with low-cost export strategy. And to have an upper position in negotiation with these vendors and to secure stable business, Suncheon Nonghyup needs to make its own efforts, linked exports between NH Nonghyup trade and the same industry, as well as co-related strategies among exporters to fortify negotiation power and response of Korean Kimchi industry. Namdo Food needs to create synergy by facilitating wide exchanges and forming practical infrastructure links among existing businesses.

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