模型을 이용한 CT번호의 변화에 關한 實驗的 硏究

김인원 서울大學校 大學院 1986 국내박사

通玄二八丹의 Methicillin-resistant Staphylococcus aureus에 대한 항균활성

김인원 圓光大學校 2015 국내박사

Methicillin-Resistant Staphylococcus aureus (MRSA)는 원내감염의 주요원인균으로 널리 알려져 있다. 갈수록 감염률이 높아지고 항생제에 대한 내성이 높아지면서 세계적으로 심각한 문제를 일으키고 있다. 새로운 항생제가 발견되더라도 세균의 내성 발현 속도가 빠르기 때문에 기존의 항생제의 적절한 활용과 내성을 최소화시킬 수 있는 연구가 필요하다. 이에 본 연구에서는 전통 한약 처방인 통현이팔단의 에탄올 추출물의 MRSA에 대한 항균활성을 측정하였다. 실험에서는 Disc 확산법, 최소억제농도 (minimum inhibitory concentration ; MIC), 약물병용효과 (FICI)와 시간별 균의 생육곡선 (Time-kill)을 확인하였다. 더 나아가 S. aureus 에 대한 통현이팔단의 작용기전에 대하여 연구를 진행하였다. cell membrane의 투과성을 측정하기 위해, 통현이팔단 500ug/mL의 농도로 Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (TRIS)와 병용실험을 실시하였다. N,N-dicyclohexylcarbodimide (DCCD)는 ATPase의 억제제로 사용하였다. 약물병용실험에서는 통현이팔단 에탄올 추출물과 상용항생제인 AM, OX, VC, GT의 병행처리 시의 항생제 증강효과에 대하여 탐색하였다. 그 결과, 통현이팔단 에탄올 추출물 (THD)에서 모든 균주에 대하여 1,000~2,000 ug/mL의 MIC를 확인하였고 우수한 항균효과가 있음을 확인하였다. 또한 기존의 항생제인 Ampicillin, oxacillin, Gentamicin과 병용투여결과 대부분 FICI가 0.5이하의 수치를 나타냄으로써 배합처리로 인한 효과상승을 유도하여 S. aureus 균주의 항생제 감수성 균주 및 내성 균주에 대한 탁월한 항균 활성을 나타내었다. Time-kill test를 통해 THD와 항생제의 시간에 따른 억제양상을 확인했고, 24h에서 완벽히 억제됨을 확인하였다. 통현이팔단과 TX, TRIS, DCCD, NaN3를 병용하였을 때 S. aureus 에 대해 상승작용을 나타내었으며, 이로써 통현이팔단의 항균작용은 cell membrane과 ATPase 억제에 영향을 준다는 것이 확인되었다. 이로써 MRSA에 대한 통현이팔단 에탄올 추출물은 항균활성을 가지고 있으며, 천연 항생제의 후보물질로써 충분히 가능성을 가지고 있음을 시사하는 바이다. Methicillin-resistant Staphylococcus aureus (MRSA) is a human pathogen and a major cause of hospital-acquired infections. New antibacterial agents that have not been compromised by bacterial resistance are needed to treat MRSA-related infections. This study investigated the antibacterial activity of EtOH 70% extracts of Tonghyeonipaldan (THD) which prescription is composed of oriental medicine against MRSA. The antibacterial activity of THD extract was evaluated against MRSA strains by using the Disc diffusion method, broth microdilution method (minimal inhibitory concentration; MIC), Checkerboard dilution test, and Time-kill test; its mechanism of action was investigated by bacteriolysis, detergent or ATPase inhibitors were used. The checkerboard dilution test was used to examined synergistic effect of Ampicillin, Oxacillin, Gentamicin and Vancomycin in combination with THD ethanol extract. Furthermore, a time-kill assay was performed a survival curve which was obtained by plotting viable colony counts depending on time on bacterial growth. The minimum inhibitory concentration (MIC) of Tonghyeonipaldan ethanol extract (THD) is 1,000~2,000 ug/mL against all the tested bacterial strains, respectively. We able to confirm that THD extract has potentially strong antibacterial activity. In the checkerboard dilution test, fractional inhibitory concentration index (FICI) of THD in combination with antibiotics indicated synergy or partial synergism against S. aureus. Furthermore, a time-kill assay showed that the growth of the tasted bacteria was considerably inhibited after 24 hr of treatment with the combination of THD with selected antibiotics. For measurement of cell membrane permeability, THD 500 ug/mL along with concentration of Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (TRIS) were used. In the other hand, N,N-dicyclohexylcarbodimide (DCCD) and Sodium azide(NaN3) were used as an inhibitor of ATPase. TX, TRIS, DCCD and NaN3 cooperation against S. aureus showed synergistic action. Accordingly, antimicrobial activity of THD was affected by cell membrane and inhibitor of ATPase were assessed. These results suggest that ethanol extract of Tonghyeonipaldan has antibacterial activity, and that THD extract offers great potential as a natural antibiotic against MRSA.

정신지체 특수학교 직업교육 실태와 교사·부모 인식

김인원 대구대학교 2011 국내석사

財産稅制의 投機抑制效果에 關한 硏究

김인원 建國大學校 1989 국내석사

기계층버섯 균사체로 발효한 나물콩의 생리활성 및 식품 적용

김인원 전북대학교 일반대학원 2018 국내박사

To expand the utilization of domestic sprout soybeans that can not be used as sprouts, among the physiologically active substances present in sprout soybeans, the conversion to aglycone, a physiologically active form of isoflavone which acts as a vegetable estrogen, was carried out by using the mushroom mycelium. Irpex lacteus mycelia, which showed fast activity and relatively high level of isoflavone bioactive conversion in sprout soybean liquid medium, was used as a spawn for a sprout soybean solid culture. Beans were treated with steaming(SS), germinating(GS), roasting(RS) and fermented with I. lacteus mycelia 10%(v/w) for 20 days to enhance biological activities of their sprouts. The total phenolic contents, flavonoids, isoflavone, and DPPH(2,2-diphenyl-1-picrylhydra zyl) radical scavenging activity of each fermented bean were examined every 5 days for 20 days(25℃, 80%). For the application to food, free amino acid, β-glucan contents, antioxidative activity by luminol method, and cytotoxicity were measured for sprout soybeans fermented with I. lacteus mycelia on the 5th day. The quality characteristics of soymilk were investigated using sprout soybeans powder fermented with I. lacteus mycelia. The total phenolic, flavonoid, isoflavone, and radical scavenging activity of each fermented bean preparation were examined every 5 days for 20 days. The total phenolic content of SS, GS, and RS preparations was 9.61, 10.23, and 10.46 mg/g, respectively, after 15 days of fermentation. These concentrations were approximately 4-5 folds higher compared to initial levels. The total flavonoid content was 2-4 folds higher than initial levels. Of all the treatments, the isoflavone content was highest in the RS sample(6.84 mg/g). The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of beans fermented with I. lacteus mycelia was increased 2-8 folds after 20 days of fermentation. Antioxidant activity components were increased by fermentation of I. lacteus mycelia irrespective of soybean treatments. The correlation coefficients between antioxidant activity(DPPH) and total phenol content(TPC) was highest in all the treatments. The β-glucan contents of beans fermented with I. lacteus mycelia was increased 4∼5 folds after 5th day of fermentation, SS(3.16%), GS(3.11%), and RS(3.20%). Free amino acids were detected 23 species, there were no difference in contents according to processing method, amino acid contents of the sprout soybeans fermented with I. lacteus mycelia after 5th days, increased from 5.69 mg/g to 19.11 mg/g(SS), from 5.68 mg/g to 18.91 mg/g(GS), from 4.16 mg/g to 23.57 mg/g(RS). Volatile components were identified by Puge & trap method, SS isolated forty fractions, SS fermented with I. lacteus mycelia after 5th days(SSF) isolated forty one, GS isolated forty fractions, GS fermented with I. lacteus mycelia after 5th days(GSF) isolated forty five, RS isolated twenty-five fractions, RS fermented with I. lacteus mycelia after 5th days(RSF) isolated thirty fractions. Volatile components of sprout soybean fermented with I. lacteus mycelia decreased the ratio of 2-ethyl furan to n-hexanal, 1-hexanal, propanone, propanol and benzaldehyde by fermented mushroom mycelia, 1-octen-3-ol, 3-octanol and 5-undecene, which are typical fragrance components of mushrooms, were increased. Cell viability rate(%) investigated inhibitory effect on HT29 cell, human colorectal carcinoma cell and HepG2, human hepatocellular carcinoma cells in CCK-8 assay of sprout soybeans fermented with I. lacteus mycelia. Cell viability rate(%) in HT29 cell were significantly lower than that of blank. Cell viability rate(%) in HepG2 cell were higher than that of blank. In general, the inhibitory effect on HT29 cell of sprout soybean fermented with I. lacteus mycelia were confirmed in all samples, and cell viability was decreased with increasing concentration. Soymilk was manufactured adding sprout soybean powder fermented with I. lacteus mycelia, which was determined to be adequate in terms of isoflavone contents and sensory testing by the 7 point scale method(P<0.05), with a 10% powder added. Based on the above results, it is expected to use of I. lacteus mycelia as a new material of mushroom mycelium which gives fast growth, increase of physiological activity and change of volatile components in sprout soybean media. The concentration of the liquid-media for inoculation in the sprout soybean solid medium was obtained by inoculating a mycelium 10%(v/w), which was activated in the liquid medium for the secondary time, and cultured at 25℃, 80% for 5 days. The antioxidant activity of roasted soybeans was higher in the physiological activity test by applying the mycelium to the different processing methods(steaming, germinating, and roasting) of the soybeans. More researches of the activity of various enzymes in I. lacteus mycelia such as how to apply the processing method to food materials are needed. In addition, the future research in the application to various foods and the toxicity test for normal cells will be also needed.

서구 미술 경매제도를 통해 본 한국 미술시장에서 경매제도의 정착 방안

김인원 서울대학교 대학원 2007 국내석사

行政上의 損失補償制度에 관한 硏究

김인원 成均館大學校 1985 국내석사

지능형 영상보안시스템의 수용의도 영향 요인에 관한 연구

김인원 숭실대학교 대학원 2017 국내박사

The frequent occurrence of traditional machine security systems is generating conflicts between the police and users and providers have creeping costs problems due to frequent responses. The current machine security systems simply combined with an intrusion alarm system, an access control system, and an image monitoring system have increased the security, safety, and criminal arrest ratio but the profitability of providers deteriorated considerably due to burden of investment costs. Therefore, industries have badly needed a development of new type of security system. The intelligent video security system appearing in current situation is growing its interest because it is expected to be adopted the emerging artificial intelligence. However, there is a lack of theoretical definition and researches on acceptance intention. This study is aiming to consider influential factors on acceptance intention of intelligent video security system synthetically to consolidate Information System Success Model by DeLone & McLean(2003) and Value-based Adoption Model by Kim et al.(2007). This study assumed that users recognize the intelligent video security system as an information equipment for security and safety in the analysis process of acceptance intention of intelligent video security system. Based on this realization, the major factors related with D&M IS model were analyzed to apply this study. Moreover, the intelligent video security system user was considered as a user who is responsible for expenses spontaneously as occasion demands and VAM was used for analysis to consider an aspect of Benefits and an aspect of Sacrifices from new technology use simultaneously. The study hypothesis was established to verify empirically and SPSS 22.0 and AMOS 22.0 were used for analysis. The results were as follows. First, the Benefits factors such as information quality of D&M IS model, system quality, and service quality had positive effect on acceptance intention of intelligent video security system. Second, the recognized security and the perceived expense among Sacrifices factors such as a privacy concern, recognized security, and the perceived expense did not have any effect due to rejecting hypothesis and the privacy concern had a negative effect by adopting hypothesis. Third, perceived value had a positive effect on both user satisfaction and acceptance intention and the user satisfaction had also a positive effect on acceptance intention. The most influential factor to acceptance intention of intelligent video security system was the system quality because an image analysis function could be a core factor to decide quality of intelligent video security system. Therefore, correct and detailed image analysis program should be developed for adoption of intelligent video security system by consumers. This study has a scientific meaning because it suggested new approach method combined with D&M IS model and VAM for a field of influential effect to acceptance intention of intelligent video security system. According to the study on influential effect to acceptance intention of intelligent video security system, the results from the study combined two models showed that the hypothesis of privacy concern was adopted as opposed to the information system adopted by organizations. It showed that the invasion of consumer's privacy should be considered. The results of this study would offer various implications for providers who want to offer service by using the intelligent video security system and workers who study related technology. If the intelligent video security system is commercialized in earnest, overinvesting costs of machine security system and structural problems of incorrect or frequent alarms would be solved. In addition, consumer utility could be increasing more because of lower cost and more safe state-of-the art security. 전통적인 기계경비시스템은 오·경보가 자주 발행하여 경찰 및 사용자와의 갈등요인으로 작용하였고 제공 사업자는 잦은 출동으로 인해 비용이 늘어나는 문제가 있었다. 침입경보시스템, 출입통제시스템 및 영상감시시스템이 단순하게 결합된 현재의 기계경비시스템은 보안·안전 및 범인 검거 비율은 향상되었지만 제공 사업자의 투자비 부담이 가중되어 수익성은 크게 악화 되었다. 이에 따라 업계에서는 새로운 방식의 보안시스템 개발이 절실하였다. 이러한 환경에서 등장한 지능형 영상보안시스템은 최근 새롭게 대두되고 있는 인공지능이 가장 먼저 적용될 산업으로 전망되면서 관심이 증가하고 있으나 이론적 정의와 수용의도에 관한 연구는 부족한 것이 사실이다. 이에 본 연구는 이러한 부족한 연구를 메우기 위해 DeLone & McLean(2003)의 정보시스템 성공모형(Information System Success Model)과 Kim et al.(2007)의 가치기반 수용모델(Value-based Adoption Model)을 통합하여 지능형 영상보안시스템의 수용의도에 영향을 미치는 요인에 대하여 종합적으로 고찰하는데 목적이 있다. 본 연구는 지능형 영상보안시스템의 수용의도를 분석하는 과정에서 이용자들은 지능형 영상보안시스템을 보안·안전에 필요한 정보기기로 인식한다고 가정하였다. 이와 같은 인식을 바탕으로 D&M IS 모형에 관련된 주요 요인들을 분석하여 본 연구에 적용하였다. 또한 지능형 영상보안시스템의 이용자를 개인이 필요에 따라 자발적으로 비용을 부담하는 소비자로 간주하고 신기술 사용으로 얻게 되는 편익(Benefits) 측면과 희생(Sacrifices) 측면을 동시에 고려하고자 VAM을 활용하여 분석하였다. 연구 과제를 실증적으로 검증하기 위해 연구가설을 설정하고 SPSS 22.0과 AMOS 22.0을 활용하여 분석한 결과 첫째, D&M IS 모형의 정보 품질, 시스템 품질, 서비스 품질 등 편익 요인은 지능형 영상보안시스템의 수용의도에 긍정적인 영향을 미치는 것으로 나타났다. 둘째, 프라이버시 염려, 인지된 보안, 지각된 비용 등의 희생 요인 중에서 인지된 보안과 지각된 비용은 가설이 기각되어 영향이 없는 것으로 확인되었고, 프라이버시 염려는 가설이 채택되어 부정적인 영향을 미치는 것으로 나타났다. 셋째, 지각된 가치는 사용자 만족과 수용의도에 모두 긍정적인 영향을 미치는 것으로 나타났고, 사용자 만족 또한 수용의도에 긍정적인 영향을 미치는 것으로 확인되었다. 특히, 지능형 영상보안시스템의 수용의도에 가장 큰 영향을 미치는 요인은 시스템 품질로 나타났다. 이와 같은 연구결과가 도출된 이유는 영상분석 기능이 지능형 영상보안시스템의 품질을 결정하는 핵심요인으로 볼 수 있기 때문이다. 따라서 지능형 영상보안시스템이 소비자에게 성공적으로 수용되기 위해서는 다른 무엇보다 정확하고 정교한 영상분석 프로그램 개발이 선행되어야 할 것이다. 본 연구는 D&M IS 모형과 VAM을 통합하여 지능형 영상보안시스템의 수용의도에 영향을 미치는 요인에 대한 연구 분야에서 새로운 접근방법을 제시했다는 점에서 학술적 의의가 있다. 두 가지 모델을 통합하여 연구한 결과 지능형 영상보안시스템의 수용의도에 영향을 미치는 요인에 관한 연구에서는 조직에서의 정보시스템 도입과는 다르게 프라이버시 염려라는 가설이 채택되어 소비자의 사생활 침해에 대한 고려가 필요하다는 것을 알 수 있었다. 이와 같은 결과는 지능형 영상보안시스템을 활용하여 서비스를 제공 하고자 하는 사업자나 관련 기술을 연구하는 실무자에게 많은 시사점을 제공할 것이다. 그리고 지능형 영상보안시스템이 본격적으로 상용화 된다면 기계경비시스템의 과도한 투자비와 구조적인 오·경보 문제도 극복될 것으로 기대한다. 또한 소비자는 보다 안전한 첨단 보안 서비스를 보다 저렴한 가격으로 이용할 수 있어 소비자 효용이 더욱 증가할 것으로 기대한다.

옻나무로부터 抗酸化活性 物質의 同定 및 油脂相에 따른 抗酸化作用

김인원 全北大學校 1999 국내석사

오스테나이트계 고 N 함유 Cr-Mn-Ni-N강의 미세구조 및 고온변형 특성

김인원 忠南大學校 에너지科學技術大學院 2016 국내석사

Austenitic heat resistant steel STR35 for exhaust valves of automobile engines have good heat resistibility as well as oxidation due to precipitation hardening and its chemical composition. The hardness in STR35 is increasing with aging time, reaching a maximum, followed by the hardness decrease on overaging. M23C6 carbides are initially precipitated while M23C6 and M6C carbides with aging time, and elogation and reduction of area are decreasing. In this study, Hot compression test in the temperature range of 750~1250ㅇC and strain rate of 5mm/sec, 20mm/sec and 100mm/sec. The shape of flow stress curves indicated that the softneing was promoted by dynamic recovery followed by dynamic recrystallization whit high level of recovery. The microstructure evolution indicated that even though after large straining as that of the steady state stresses a combination of strained and recrystallized grains was observed.