점농어 , Lateolabrax maculatus 난소에서 생성되는 C21 - 스테로이드 = Biosynthesis of C21 - steroids in Spotted Sea Bass ( Lateolabrax maculates ) Ovaries

국문 초록 (Abstract)

점농어의 난소성숙과 배란과정에서 생성되는 성 스테로이드 호르몬 특히, C_(21)-스테로이드를 분석하고자 전구물질 ³H-pregneolone와 ³H-17αhydroxyprgesterone를 난소조직 배양초기에 첨가하여 24시간 배양 뒤 생성된 물질은 성숙기 난소 (난경 700∼800㎛)의 경우 corticosteroids계의 호르몬과 17α20βOHP가 관찰되었으나 17α20β 0HP만이 HPLC상에서 확인되었다. 특히 배란중인 점농어에서는 17α20βOHP, 17α20β21p 그리고 corticosterone의 존재가 뚜렷하였다.
따라서 점농어에서는 난소의 성숙과 배란과정에 17α20βOHP이 모두 관여하며, 배란에 이르면서 17α20β21P의 출현이 뚜렷한 것으로 생각된다. corticosterone의 존재에 대해서는 이 물질이 점농어의 배란과 관련이 있는지 또는 스트레스 조건에서 나타나는 순간적인 생성물인지 앞으로 더욱 상세한 분석방법으로 접근해야 할 부분이다.

다국어 초록 (Multilingual Abstract)

To investigate the production of C_(21)-steroids during the spawning period of spotted sea bass, Lateolabrax maculatus, we have incubated maturing and ovulating follicles with radiolabeled pregnenolone and 17α-hydroxyprogesterone for 24 hours. The resulting metabolites were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). When maturing follicles (700∼800㎛ in diameters) were incubated with radiolabeled precursors, C_(21)-metabolites were corticosteroids and 17α-hydroxy,20β-dihydroprogesterone (17α20βOHP). When ovulation follicles (1,000∼1,150 ㎛ in diameters) were incubated with radiolabeled precursors, the major C_(21)-metabolites were 17α20βOHP, 17α20β,21-trihydroxy-4-pregnen-3-one (17α20β21P), and corticosterone. Additional chromatography by TLC and HPLC confirmed the presence of radioactive 17α20βOHP in the maturing follicles, and 17α20βOHP, 17α20β21P and corticosterone in ovulating follicles. Although 17α20βOHP was found in a small peak, the synthesis of this steroid suggests that it may play a role in regulating the oocyte maturation process. Whereas ovulation is regulated by both 17α20βOHP and 17α20β21P in the spotted sea bass. In addition, an unusual finding was the biosynthesis of corticosterone. Whether this production is responsible for the ovulation, and is an area requiring continued research.