This experiment was carried out to determine the effect of kinds amino acids and concentrations on shoot growth of roses (Rosa hybrida) by nodal culture. The results from this study were summarized as follows ;
1.The addition of proline into culture ...
This experiment was carried out to determine the effect of kinds amino acids and concentrations on shoot growth of roses (Rosa hybrida) by nodal culture. The results from this study were summarized as follows ;
1.The addition of proline into culture medium was more effective for subsequent shoot growth in vitro rather than that of casein hydrolysate or glutamine and more leaves were also formed by proline.
2.Subsequent shoot growth and leaf formation both were more stimulated by increase in concentrations of 3 tested amino acids. Among 3 amino acids tested, proline at 500mg /ℓ was determined optimal for subsequent shoot growth. The number of leaves were increased as the concentrations of proline and glutamine were increased.
3.Shoot multiplication was not affected by any type and concentration of amino acids tested in this study.