Preparative Isolation of 8-Methoxypsoralen from the Rhizomes of Pulsatilla chinensis using High-speed Counter-current Chromatograpy

다국어 초록 (Multilingual Abstract)

A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.

참고문헌 (Reference)

1 Mimaki Y, "Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells" 62 : 1279-1283, 1999

2 Zic JA, "The treatment of cutaneous T-cell lymphoma with photopheresis" 16 : 337-346, 2003

3 Bae KH, "The Medicinal Plants of Korea" Kyo-Hak 139-, 2000

4 Sutherland IA, "Role of counter-current chromatography in the modernization of Chinese herbal medicines" 1216 : 740-753, 2009

5 Ye WC, "Pulsatilloside C from the roots of Pulsatilla chinensis" 61 : 658-659, 1998

6 Knobler RM, "Psoralen Phototherapies, In Psoralen DNA Photobiology, II" CRC Press 117-134, 1988

7 Wollina U, "Pompholyx:A review of clinical features,differential diagnosis,and management" 11 : 305-314, 2010

8 Ye WC, "New lupane glycosides from Pulsatilla chinensis" 68 : 183-186, 2001

9 Mimaki Y, "New bisdesnosidic triterpene saponins from the roots of Pulsatilla chinensis" 64 : 1226-1229, 2001

10 Abu-Mustafa EA, "Natural coumarins.VI.Nuclear magnetic resonance spectra of some coumarin and coumarilic acid derivatives" 45 : 325-327, 1967

11 Slacanin I, "Modifications to a high-speed counter-current chromatography for improved separation capability" 482 : 234-239, 1989

12 Edelson RL, "Light-activated drugs" 259 : 68-75, 1988

13 Conway WD, "Development of countercurrent chromatography" 56 : 534-554, 1984

14 Pauli GF, "Countercurrent separation of natural products" 71 : 1489-1508, 2008

15 Ito J, "Countercurrent chromatography:Liquid-liquid chromatography without solid support" 167 : 281-283, 1970