Kinetic Evaluation of Products Inhibition to Succinic Acid Producers Escherichia coli NZN111, AFP111, BL21, and Actinobacillus succinogenes 130ZT

다국어 초록 (Multilingual Abstract)

Succinic acid is one of the platform compounds and its production via natural feedstocks has drawn worldwide concerns. To evaluate the inhibitory effects of fermentation products on the growth of Actinobacillus succinogenes 130ZT and Escherichia coli NZN111, AFP111, BL21, fermentations with addition of individual products in medium were carried out. The cell growth was inhibited when the concentrations of formate, acetate, lactate, and succinate were at range of 8.8-17.6 g/L, 10-40 g/L, 9-18 g/L, and 10-80 g/L, respectively. For these two species of bacteria, E. coli was more resistant to acid products than A. succinogenes, while both endured succinate rather than by-products. As a result of end product inhibition, succinate production yield by A. succinogenes decreased from 1.11 to 0.49 g/g glucose. Logistic and Monod mathematical models were presented to simulate the inhibition kinetics. The Logistic model was found more suitable for describing the overall synergistic inhibitory effects.

참고문헌 (Reference)

1 Park, D.H., "Utilization of electrically reduced neutral red by Actinobacillus succinogenes: physiological function of neutral red in membrane-driven fumarate reduction and energy conservation" 181 : 2403-2410, 1999

2 Peleg, M., "The logistic (Verhulst) model for sigmoid microbial growth curves revisited" 40 : 808-818, 2007

3 Delhomme, C., "Succinic acid from renewable resources as a C4 building-block chemical-a review of the catalytic possibilities in aqueous media" 11 : 13-26, 2009

4 Vemuri, G.N., "Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions" 28 : 325-332, 2002

5 Lin, C.S.K., "Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes" 41 : 128-135, 2008

6 McKinlay, J.B., "Prospects for a biobased succinate industry" 76 : 727-740, 2007

7 Sánchez, A.M., "Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity" 7 : 229-239, 2005

8 Song, H., "Modeling of batch fermentation kinetics for succinic acid production by Mannheimia succiniciproducens" 40 : 107-115, 2008

9 Guettler, M.V., "Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants. US Patent 5,573,931"

10 Lee, S.L., "Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation" 71 : 7880-7887, 2005

11 Yang, X.P., "Mathematical modeling of inhibition kinetics in acetone-butanol fermentation by Clostridium acetobutylicum" 10 : 532-538, 1994

12 Corona-González, R.I., "Kinetic study of succinic acid production by Actinobacillus succinogenes ZT-130" 43 : 1047-1053, 2008

13 Colin, T., "Inhibition of Clostridium butyricum by 1, 3-propanediol and diols during glycerol fermentation" 54 : 201-205, 2000

14 Phue, J.N., "Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21 evaluating transcription levels of key genes at different dissolved oxygen conditions" 7 : 353-363, 2005

15 Kang, H.C., "Growth inhibition of a phytopathogenic fungus, Colletotrichum species by acetic acid" 158 : 321-326, 2003

16 Lee, S.J., "Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production" 72 : 1939-1948, 2006

17 Lee, S.Y., "From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens" 79 : 11-22, 2008

18 Urbance, S.E., "Evaluation of succinic acid continuous and repeat-batch biofilm fermentation by Actinobacillus succinogenes using plastic composite support bioreactors" 65 : 664-670, 2004

19 Goncalves, L.M.D., "Elucidation of the mechanism of lactic acid growth inhibition and production in batch cultures of Lactobacillus rhamnosus" 48 : 346-350, 1997

20 Oliva, J., "Effects of acetic acid, furfural and catechol combinations on ethanol fermentation of Kluyveromyces marxianus" 41 : 1223-1228, 2006

21 Andersson, C., "Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli" 23 : 381-388, 2007

22 Chen, C.C., "Coupled lactic acid fermentation and adsorption" 59 : 170-174, 2002

23 Wee, Y.J., "Continuous production of succinic acid by a fumarate-reducing bacterium immobilized in a hollow-fiber bioreactor" 98 (98): 1093-1104, 2002

24 Luque, R., "Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method" 11 : 193-200, 2009

25 Kacena, M.A., "Bacterial growth in space flight: logistic growth curve parameters for Escherichia coli and Bacillus subtilis" 51 : 229-234, 1999

26 Okino, S., "An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain" 81 : 459-464, 2008

27 Guettler, M.V., "Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen" 49 : 207-216, 1999

28 McKinlay, J.B., "13C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO3 and H2 concentrations" 10 : 55-68, 2008