돼지 초기배 체외수정란 이식으로 산자 생산 = Production of Piglet Derived from In Vitro Produced Porcine Early Embryos

다국어 초록 (Multilingual Abstract)

It is not easy for porcine embryos produced by in vitro systems to develop into blastocysts with high quality. To solve this problem, many researchers have developed novel culture methods. However, the formation of blastocysts with high quality is still low. In this study, we aimed to produce piglet following transfer of in vitro produced early embryos ( cell stage embryos) or morula and blastocyst. The cell stage embryos were transferred to five estrus-synchronized recipients (200 embryos per recipient). One of the five sows farrowed three piglets, which contain two live piglets and one dead piglet, 114 days after embryo transfer. However, two recipients transferred with morula and blastocysts did not farrow. Microsatellite analysis confirmed that the genomic DNA of two live piglets were not genetically identical to that of the recipient. These results indicate that it is possible to obtain piglets by transfer of early embryos produced by in vitro production (IVP) systems.

참고문헌 (Reference)

1 손동수, "재래돼지에서 수정란의 회수 및 동결보존에 관한 연구" 한국수정란이식학회 19 (19): 257-264, 2004

2 손동수, "돼지의 수정란이식에 관한 고찰" 3 : 6-12, 1988

3 Hunter RHF, "The effect of superovulation on fertilization and embryonic survival in the pig" 8 : 457-465, 1966

4 Christenson RK, "Synchronization of ovulation in superovulated gilts" 31 : 219-, 1970

5 Funahashi H, "Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization" 57 : 49-53, 1997

6 Misumi K, "Successful production of piglets derived from morulae and early blstocysts using a microdroplet method" 60 : 253-260, 2003

7 Suzuki M, "Successful piglet production by IVF of oocytes matured in vitro using NCSU-37 supplemented with fetal bovine serum" 65 : 374-386, 2006

8 Kikuchi K, "Successful piglet production after transfer of blastocysts produced by a modified in vitro system" 66 : 1033-1041, 2002

9 Martinez EA, "Successful non-surgical deep uterine embryo transfer in pigs" 61 : 137-146, 2004

10 Yoshioka K, "Production of piglets derived from in vitro-produced blastocysts fertilized and cultured in chemically defined media: Effects of theophylline, adenosine, and cysteine during in vitro ferilization" 69 : 2092-2099, 2003

11 Funahashi H, "Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes" 54 : 1412-1419, 1996

12 Guthrie HD, "Plasma hormone levels and fertility in pigs induced to superovulate with PMSG" 41 : 361-370, 1974

13 Beebe FS, "Piglets born from vitrified zona-intact blastocysts" 57 : 2155-2165, 2002

14 Berthelot F, "Piglets born after vitrification of embryos using the open pulled straw method" 41 : 116-124, 2000

15 김인덕, "Open Pulled Straw(OPS) 방법에 의한 체외 배양 동결 수정란의 미경산돈 이식" 한국수정란이식학회 23 (23): 217-222, 2008

16 Marchal R, "Meiotic and developmental competence of prepubertal and adult swine oocytes" 56 : 17-29, 2001

17 Dziuk PJ, "Intra-uterine migration and mixing of embryos in swine following egg transfer" 23 : 27-42, 1964

18 Kvansnickii AV, "Interbreed ova transplantation" 1 : 36-42, 1951

19 Nagai T, "In-vitro fertilization of pig oocytes by frozen boar spermatozoa" 84 : 585-591, 1988

20 Yoshida M, "In vitro fertilization of pig oocytes matured in vivo" 49 : 711-718, 1987

21 Yoshida M, "Improved viability of two-cell stage pig emryos resulting from in vitro fertilization of oocytes matured in vivo" 35 : 35-37, 1989

22 Hancok JL, "Egg transfer in the sow" 4 : 195-201, 1962

23 Wiemer KE, "Effect of maturation and co-culture treatments on the developmental capacity of early bovine embryos" 30 : 330-338, 1991

24 Mattioli M, "Developmental competence of pig oocytes matured and fertilized in vitro" 31 : 1201-1207, 1989

25 Baker RD, "Control of ovulation rate and fertilization in prepuberal gilts" 27 : 1607-1610, 1968

26 Webel SK, "Control of estrus and ovulation in the pig by ICI 33828 and gonadotropins" 30 : 791-794, 1970

27 Suzuki C, "Birth of piglets through the non-surgical transfer of blastocysts produced in vitro" 50 : 487-491, 2004

28 Day BN, "Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y bearing spermatozoa stored by high sped flow cytometry" 49 : 360-, 1998

29 Yoshida M, "Birth of piglets devived from in vitro fertilization of pig oocytes matured in vitro" 39 : 1303-1311, 1993

30 Dobrinsky JR, "Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification" 62 : 564-570, 2000

31 Funahashi H, "Advances in in vitro production of pig embryos" 52 : 271-283, 1997