Protective Effects of Grifola frondosa Extract Containing Naringin, Hesperidin, Chlorogenic Acid, and Kaempferol on Bisphenol A-induced Apoptotic Cell Death in Normal Human Dermal Fibroblasts

다국어 초록 (Multilingual Abstract)

Grifola frondosa (GF), a species of Basidiomycotina, is widely distributed in Asia and has been used as an immunomodulatory, anti-bacterial, and anti-cancer agents. In the present study, we investigated the skin protective effect of GF extract in normal human dermal fibroblast (NHDF). We found that GF extract (100 ㎍/mL) has inhibitory effect on the cell death induced by an ecotoxicological endocrine disrupting chemical, bisphenol A (BPA) in NHDF. GF extract containing of naringin, hesperidin, chlorogenic acid, and kaempferol revealed by UPLC analysis inhibits the production of intracellular reactive oxygen species (ROS) responsible for the distinctive activations of the extracellular signal-regulated kinase (ERK) and nuclear factor-kappa B (NF-κB) in BPA-stimulated NHDF. In addition, we found that GF extract inhibited the expression of apoptosis related-proteins (Bax, Bcl-2, and cleaved caspase-3) in BPA-treated NHDF. These results indicate that GF extract is functional pharmacological agent that prevents the apoptotic cell death induced by BPA via the inhibition of ROS-mediated signaling events in human dermal fibroblast.