Clonorchis sinensis infestation induces 3-dimensional invasion of cholangiocarcinoma

다국어 초록 (Multilingual Abstract)

Clonorchis sinensis (C. sienensis) is a worm parasite that is prevalent in East Asian countries, and it causes chlonorchiasis, diverse symptoms occurred by infection of C. sinensis. Since epidemiological correlation between C. siensnsis infestation and CCA was reported, initiation of CCA involved in C. sinensis has been researched in many experimental research cases such as current studies suggesting that excretory-secretory products (ESPs), molecules that are secreted by this parasite and released into surround hepatobiliary tissue, play important role in host-parasite interaction related to CCA initiation, whereas progress of CCA has not been. Now, I investigated the possibility that C. sinensis infestation affects on poor prognosis of CCA by inducing the invasion of CCA. In the CCA cells with ESPs treatment, expression of focal adhesion proteins and secretion of matrix metalloproteinases (MMPs), related to mobility of the cells, were increased. Furthermore, Using an advanced CCA culture models based on a novel microfluidic assay, I quantitatively addressed the role of ESPs gradients from C. sinensis in promoting the invasion of CCA cells into neighboring ECM.

목차 (Table of Contents)

• ABSTRACT i
• Table of Contents iii
• List of Figures iv
• List of Tables viii
• Introduction 1
• 1.1 Clonorchis sinensis (C. sinensis) 1
• 1.2 Cholangiocarcinoma (CCA) 2
• 1.3 In vitro assay for CCA invasion related to C. sinensis infestation 3
• Materials and Methods 5
• Results and Discussion 12
• 3.1 3-dimensional culture of biliary cells 12
• 3.2 Advanced cholangiocarcinoma culture models 16
• 3.3 Invasion of CCA by C. sinensis infestation 20
• 3.4 Diffusion profile of ESPs in microfluidic device 23
• 3.5 Molecular change in CCA by C. sinensis infestation 25
• Conclusion 28
• Supplementary information 29
• Reference 32