Bisdemethoxycurcumin (BDMC) was a natural curcuminoid with many bioactivities present in turmeric (Curcuma longa L.). However, the disposition mechanisms of BDMC via uridine 5′‐diphospho‐glucuronosyltransferase (UGT) metabolism still remain uncl...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=O120592130
2018년
-
0951-6433
1872-8081
SCIE;SCOPUS
학술저널
558-569 [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
Bisdemethoxycurcumin (BDMC) was a natural curcuminoid with many bioactivities present in turmeric (Curcuma longa L.). However, the disposition mechanisms of BDMC via uridine 5′‐diphospho‐glucuronosyltransferase (UGT) metabolism still remain uncl...
Bisdemethoxycurcumin (BDMC) was a natural curcuminoid with many bioactivities present in turmeric (Curcuma longa L.). However, the disposition mechanisms of BDMC via uridine 5′‐diphospho‐glucuronosyltransferase (UGT) metabolism still remain unclear. Therefore, we aimed to determine the potential efflux transporters for the excretion of BDMC‐O‐glucuronide. Herein, chemical inhibition assays (Ko143, MK571, dipyridamole, and leukotriene C4) and biological inhibition experiments including stable knocked‐down of breast cancer resistance protein (BCRP), multidrug resistance‐associated proteins (MRPs) transporters were both performed in a HeLa cell line stably overexpressing UGT1A1 established previously. The results indicated that Ko143 (5 and 20 μM) caused a marked reduction in excretion rate (18.4–55.6%) and elevation of intracellular BDMC‐O‐glucuronide (28.8–48.1%), whereas MK‐571 (5 and 20 μM) resulted in a significant decrease in excretion rate (6.2–61.6%) and increase of intracellular BDMC‐O‐glucuronide (maximal 27.1–32.6%). Furthermore, shRNA‐mediated silencing of BCRP transporter led to a marked reduction in the excretion rate (21.1–36.9%) and an obvious elevation of intracellular glucuronide (24.9%). Similar results were observed when MRP1 was partially silenced. In addition, MRP3 and MRP4 silencing both displayed no obvious changes on the excretion rate and intracellular levels of glucuronide. In conclusion, chemical inhibition and gene silencing results both indicated that generated BDMC‐O‐glucoside were excreted primarily by the BCRP and MRP1 transporters. © 2018 BioFactors, 44(6):558–569, 2018
CD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis
A novel moonlight function of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) for immunomodulation