Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

다국어 초록 (Multilingual Abstract)

In an effort to discover alternative antimicrobials against Listeria monocytogenes, several marine algae were screened. The methanolic extract of Ecklonia cava exhibited the highest antibacterial activity against L. monocytogenes, with the ethyl acetate (EtOAc) soluble fraction of E. cava methanolic extract having a MIC value of 256 µg/mL and a MBC value of 512 µg/mL. The MIC values of streptomycin in combination with the EtOAc fraction were markedly reduced up to 64-fold, suggesting that the antibacterial activity of the antibiotic was restored when combined with the EtOAc fraction. The interaction between streptomycin and the EtOAc fraction was assessed by fractional inhibitory concentration (FIC) indices. The combination of streptomycin and the EtOAc fraction against L. monocytogenes resulted in ∑FICmin range of 0.141 to 0.266 and ∑FICmax of 0.531 for all strains. The median ∑FIC against L. monocytogenes strains ranged from 0.172 to 0.344. Thus, synergistic ranges of FIC <1 were observed for all combinations of streptomycin and the EtOAc fraction against L. monocytogenes strains. Indeed, the median ∑FIC of streptomycin–EtOAc fraction ranged from 0.172 to 0.344, suggesting a marked synergy.

참고문헌 (Reference)

1 Lanciotti R, "Use of natural aroma compounds to improve shelflife of minimally processed fruits" 15 : 201-208, 2004

2 Abutbul A, "Use of Rosmarinus officinalis as a treatment against Streptococcus iniae in Tilapia(Oreochromis sp. )" 238 : 97-105, 2004

3 Poyart-Salmeron C, "Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes" 335 : 1422-1426, 1990

4 Eom SH, "The mechanism of antibacterial activity of phlorofucofuroeckol-A isolated from an edible alga Eisenia bicyclis against methicillin-resistant Staphylococcus aureus" 171 : 569-582, 2013

5 Hsieh MH, "Synergy assessed by checkerboard. A critical analysis" 16 : 343-349, 1993

6 Aureli P, "Susceptibility of Listeria monocytogenes isolated from food in Italy to antibiotics" 83 : 325-330, 2003

7 Kim KN, "Protective effect of Ecklonia cava enzymatic extracts on hydrogen peroxide-induced cell damage" 41 : 2393-2401, 2006

8 Bajpai VK, "Potential role of leaf essential oil and extracts of Metasequoia glyptostroboides Miki ex Huto inhibit the growth of Listeria monocytogenes spp" 35 : 289-302, 2009

9 Kang SM, "Molecular docking studies of a phlorotannin, dieckol isolated from Ecklonia cava with tyrosinase inhibitory activity" 20 : 311-316, 2012

10 National Committee for Clinical Laboratory Standards, "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically"

11 Charpentier E, "Incidence of antibiotic resistance in Listeria species" 172 : 277-281, 1995

12 이정하, "In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria" 한국조류학회I 29 (29): 47-55, 2014

13 Denny J, "Human Listeria monocytogenes infections in European opportunity for improved European surveillance" 13 : 1854-1861, 2008

14 Sherwin FR, "Food Additives" Marcel Dekker 139-193, 1990

15 엄성환, "Enhancement of Polyphenol Content and Antioxidant Activity of Brown Alga Eisenia bicyclis Extract by Microbial Fermentation" 한국수산과학회 14 (14): 192-197, 2011

16 Meletiadis J, "Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, Monte Carlo simulation analysis, and in vitro-in vivo correlation data for antifungal drug combinations against Aspergillus fumigatus" 54 : 602-609, 2010

17 Conter M, "Characterization of antimicrobial resistance of foodborne Listeria monocytogenes" 128 : 497-500, 2009

18 Clinical and Laboratory Standards Institute, "CLSI document M27-A3" CLIS 2008

19 Safdar A, "Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with systemic listeriosis at a comprehensive cancer center(1955-1997)" 41 : 483-485, 2003

20 Rahman A, "Antilisterial effect of essential oil and extracts of Poncirus trifoliate Rafin. seeds" 38 : 50-55, 2013

21 Hansen JM, "Antibiotic susceptibility of Listeria monocytogenes in Denmark 1958-2001" 113 : 31-36, 2005

22 Facinelli B, "Antibiotic resistance in foodborne listeria" 338 : 1272-, 1991

23 Choi JG, "Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp" 7 : 435-441, 2010

24 Grierson DS, "An ethnobotanical study of plants used for the treatment of wounds in the Eastern Cape, South Africa" 67 : 327-332, 1999

25 Cox S, "An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds" 17 : 205-220, 2010