A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly α-1→6 linked dextran with a low amount (3-5%) of α-1→3 branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were...
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https://www.riss.kr/link?id=A19704857
Kim, Ho Sang (The Research Center for New Bio-Materials in Agriculture,Seoul National University) ; Kim, Do Man (Department of Biochemical Engineering and Research Institute for Catalysis,Chonnam National University) ; Ryu, Hwa Ja (Department of Fine Chemical Engineering,Chonnam National University) ; John F.Robyt (Laboratory of Carbohydrte Chemistry and Enzymology,Iowa State University,USA)
2000
English
475
SCIE,SCOPUS,KCI등재
학술저널
559-563(5쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly α-1→6 linked dextran with a low amount (3-5%) of α-1→3 branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were...
A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly α-1→6 linked dextran with a low amount (3-5%) of α-1→3 branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were ligated with pGEM-3Zf(-) and transformed into E. coli DH5α. The recombinant clone (pDSRB742) synthesized dextran on an agar plate containing 2%(w/v) sucrose. The dextran synthesized was hydrolyzed with Penicillium endo-dextranase. The hydrolyzate was composed of glucose, isomaltose, isomaltotriose, and branched pentasaccharide. The nucleotide sequence of dsrB742 showed one open reading frame (ORF) composed of 4,524bp encoding dextransucrase. The deduced amino acid sequence revealed a calculated molecular mass of 168.6kDa. It also showed an activity band of 184kDa on a non-denaturing SDS-PAGE (10%). The amino acid sequence of DSRB742 exhibited a 50% similarity with DSRA from L. mesenteroides B-1299, a 70% similarity with DSRS from L. mesenteroides B-512 (F, FMCM) and a 45-56% similarity with Streptococcal GTFs.
Cation Flux-Mediated Activation of P-Type ATPase in Helicobacter pylori