신규 serum-based 항진균제 MIC 측정법에 관한 연구 = A study on new serum-based antifungal agents MIC assay

다국어 초록 (Multilingual Abstract)

In this study, serum based MIC testing was developed and optimized
to solve the limitations of the conventional CLSI-based MIC test and the
usefulness of this testing for clinical application was determined. Candida
albicans reference strain (ATCC 10231) and a total of 105 clinical
isolates and anti-fungal agents caspofugin were used for the study.
Results showed that growth characteristics were different according to
types of serum and the mouse serum was the most suitable for study.
In order to measure the optimal concentration of serum, 0% to 100%
mouse serum were added. The optimal concentration of serum was 50%
when consideration of antifungal agents administration and inoculum size,
serum components and ease of hyphae separated, and the consideration
of the degree of growth. In comparison of the usefulness between the
conventional standard CLSI-based MIC and 50% mouse serum-based
MIC testing, the range of MIC 80 of the CLSI-based MIC was 0.13 ~2.0 ㎍/mL (SD ± 0.42 ㎍/mL) and that of the serum-based MIC assay
was 1.0 ~ 16.0 ㎍/mL (SD ± 2.36 ㎍/mL). The MIC 80 of serum-based
MIC testing was increased by up to 4 to 64 times. The MICs of clinical
isolate NO, 28 in both MIC assays were 2.0 ㎍/mL and 32.0 ㎍/mL and
those of clinical isolate NO. 78 in both MIC assays were 0.50 ㎍/mL and
32.0 ㎍/mL. In conclusion, the 50% mouse serum-based MIC testing was
more useful for clinical application than conventional MIC assay.

목차 (Table of Contents)

• 제1장 서 론 ···················································································································· 1
• 제2장 연구 대상 및 방법 ···························································································· 9
• 제1절 연구 대상 ········································································································ 9
• 1. 대상 균주 ··········································································································· 9
• 제2절 연구 방법 ······································································································ 15
• 1. CLSI-based MIC 측정법 ············································································ 15
• 1) 배지 및 항진균제 준비 ············································································ 15
• 2) 실험균주 준비 ···························································································· 15
• 3) 결과 관찰 및 분석 ···················································································· 16
• 2. serum-based MIC 측정법 ········································································· 17
• 1) 배지 및 항진균제 준비 ············································································ 17
• 2) 실험균주 준비 ···························································································· 17
• 3) 결과 관찰 및 분석 ···················································································· 17
• 제3장 결 과 ·················································································································· 18
• 제1절 serum 종류에 따른 효과 분석 ································································ 18
• 제2절 최적 serum 농도 분석 ·············································································· 20
• 제3절 항진균제 감수성 측정 최적 조건 및 유효성 분석 ······························ 27
• 1. 항진균제 감수성 측정 최적 조건 ······························································· 27
• 2. 임상분리균주를 이용한 유효성 분석 ························································· 29
• 제4장 고 찰 ·················································································································· 35
• 제5장 결 론 ·················································································································· 37
• 참고문헌 ·························································································································· 39
• 요약문 ······························································································································ 43
• 감사의 글 ······················································································································ 45