인삼 뿌리썩음병균에 항균활성이 있는 방선균 BK185의 분리 및 특성

다국어 초록 (Multilingual Abstract)

Ginseng (Panax ginseng C. A. Meyer) is an economically valuable pharmaceutical crop in Korea. In order to find promising biocontrol agents for soil-borne fungal pathogens which infect ginseng roots, we have isolated actinomycete, BK185 from soil. The isolate was investigated for the antifungal activity against to ginseng rot pathogens prior to testing genetic and chemical properties. The strain was identified as Streptomyces sp. using phylogenetic analysis based on 16S rRNA gene sequence. The most closely related species was S. sporoclivatus and S. geldanamycininus with high similarities (>99%). The isolate, BK185 showed positive reaction for PCR detection targeting biosynthetic gene clusters of PKS (Type-I polyketide synthase) and NRPS (Non-ribosomal polypeptide synthetase) genes. Major metabolite from the BK185 was analyzed by The LC/MS and identified to geldamycin, which was known to contained broad antibacterial, antifungal or anticancer activities. The results provide evidences that the strain, BK185 can be promising biocontrol agent for ginseng organic farming.

참고문헌 (Reference)

1 이순구, "인삼 뿌리썩음병 관련 Fusarium species와 그 병원성" 한국식물병리학회 10 (10): 248-259, 2004

2 Saitou, N., "The neighbor-joining method: A new method for reconstructing phylogenetic trees" 4 (4): 406-425, 1987

3 Goodfellow, M., "The Streptomyces violaceusniger clade : a home for streptomycetes with rugose ornamented spores" 92 : 173-199, 2007

4 Kim, B. -Y., "Streptomyces cocklensis sp. nov., a dioxamycinproducing actinomycete" 62 (62): 279-283, 2012

5 Hopwood, D. A., "Soil to genomics: The Streptomyces chromosome" 40 : 1-23, 2006

6 Rothrock, C.S., "Role of antibiosis in antagonism of Streptomyces hygroscopicus var. geldanus to Rhizoctonia solani in soil" 30 : 1440-1447, 1984

7 Wayne, L. G., "Report of the ad hoc committee on reconciliation of approaches to bacterial systematics" 37 : 463-464, 1987

8 Tapi, A., "New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction" 85 (85): 1521-1531, 2010

9 Ayuso-Sacido, A., "New PCR primers for the screening of NRPS and PKS-I systems in actinomycetes: detection and distribution of these biosynthetic gene sequences in major taxonomic groups" 49 : 10-24, 2005

10 장창순, "Nested PCR 기법을 이용한 인삼 뿌리썩음병원균의 특이적 검출" 한국식물병리학회 11 (11): 48-55, 2005

11 Chenna, R., "Multiple sequence alignment with the clustal series of programs" 31 (31): 3497-3500, 2003

12 Seifert, K., "Molecular differentiation and detection of ginsengadapted isolates of the root rot fungus Cylindrocarpon destructans" 93 (93): 1533-1542, 2003

13 Fu, J., "Molecular detection of Cylindrocarpon destructans in infected chinese ginseng roots and soil" 11 (11): 9955-9960, 2012

14 Kumar, S., "Mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment" 5 (5): 150-163, 2004

15 Goodfellow, M., "Manual of industrial microbiology and biotechnology" ASM Press 13-27, 2010

16 Rongjun Guo, "In vitro Inhibition of Fungal Root-Rot Pathogens of Panax notoginseng by Rhizobacteria" 한국식물병리학회 25 (25): 70-76, 2009

17 DeBoer C., "Geldanamycin, a new antibiotic" 23 : 442-447, 1970

18 Toyomura K., "Effects of Hsp90 inhibitors, geldanamycin and its analog, on ceramide metabolism and cytotoxicity in PC12 cells" 37 : 1049-1057, 2012

19 Chang Soon Jang, "Direct Detection of Cylindrocarpon destructans, Root Rot Pathogen of Ginseng by Nested PCR from Soil Samples" 한국균학회 38 (38): 33-38, 2010

20 Felsenstein, J., "Confidence limits on phylogenies: An approach using the bootstrap" 39 : 783-791, 1985

21 Rascher A., "Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602" 218 (218): 223-230, 2003

22 Yelim Jang, "Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot" 한국식물병리학회 27 (27): 333-341, 2011

23 Bérdy, J., "Bioactive microbial metabolites: A personal view" 58 : 1-26, 2005