Effect of the Antimicrobial Peptide D-Nal-Pac-525 on the Growth of Streptococcus mutans and Its Biofilm Formation = Effect of the Antimicrobial Peptide D-Nal-Pac-525 on the Growth of Streptococcus mutans and Its Biofilm Formation

다국어 초록 (Multilingual Abstract)

Streptococcus mutans is the primary etiological agent of dental caries. The antimicrobial peptide D-Nal-Pac-525 was designed by replacing the tryptophans of the Trp-rich peptide Pac-525 with D-β-naphthyalanines. To assess the effect of D-Nal-Pac-525 on cariogenic bacteria, the activity of D-Nal-Pac-525 on the growth of S. mutans and its biofilm formation were examined. D-Nal- Pac-525 showed robust antimicrobial activity against S. mutans (minimum inhibitory concentration of 4 μg/ml). Using scanning electron microscopy and transmission electron microscopy, it was shown that D-Nal-Pac-525 caused morphological changes and damaged the cell membrane of S. mutans. D-Nal-Pac-525 inhibited biofilm formation of S. mutans at 2 μg/ml. The results of this study suggest that D-Nal-Pac-525 has great potential for clinical application as a dental caries-preventing agent.

참고문헌 (Reference)

1 Elbaz M, "The metabolic enzyme ManA reveals a link between cell wall integrity and chromosome morphology" 6 : e1001119-, 2010

2 Petersen PE, "The World Oral Health Report 2003: continuous improvement of oral health in the 21st century - the approach of the WHO Global Oral Health Programme" 31 : 3-24, 2003

3 Wilson M, "Susceptibility of oral bacterial biofilms to antimicrobial agents" 44 : 79-87, 1996

4 Wei SY, "Solution structure of a novel tryptophan-rich peptide with bidirectional antimicrobial activity" 188 : 328-334, 2006

5 Wu JM, "Solution structure of a novel D-β-naphthylalanine substituted peptide with potential antibacterial and antifungal activities" 88 : 738-745, 2007

6 Lemos JA, "Responses of cariogenic streptococci to environmental stresses" 7 : 95-107, 2005

7 Clinical and Laboratory Standards Institute, "Performance Standards for Antimicrobial Susceptibility Testing: Fifteenth Informational Supplement M100-S15" Wayne 2005

8 Oren Z, "Mode of membrane interaction and structure in micelles of a short a-helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy" 269 : 3869-3880, 2002

9 Yeaman MR, "Mechanisms of antimicrobial peptide action and resistance" 55 : 27-55, 2003

10 Raj PA, "Large-scale synthesis and functional elements for the antimicrobial activity of defensins" 347 : 633-641, 2000

11 Wang CW, "Increased potency of a novel D-betanaphthylalanine- substituted antimicrobial peptide against fluconazole-resistant fungal pathogens" 9 : 967-970, 2009

12 Russell RR, "How has genomics altered our view of caries microbiology?" 42 : 319-327, 2008

13 Chastre J, "Evolving problems with resistant pathogens" 14 : 3-14, 2008

14 Liu Y, "Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm" 37 : 33-38, 2011

15 Liu N, "Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli" 64 : 60-65, 2006

16 Wei GX, "Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm" 57 : 1100-1109, 2006

17 Yu HY, "Easy strategy to increase salt resistance of antimicrobial peptides" 55 : 4918-4921, 2011

18 Merrifield EL, "D-Enantiomers of 15-residue cecropin A-melittin hybrids" 46 : 214-220, 1995

19 Kolodkin-Gal I, "D-Amino acids trigger biofilm disassembly" 328 : 627-629, 2010

20 Hancock RW, "Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics" 2 : 79-83, 2002

21 Liu H, "Chitosan kills bacteria through cell membrane damage" 95 : 147-155, 2004

22 Stepanovic S, "Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface" 38 : 428-432, 2004

23 Paster BJ, "Bacterial diversity in human subgingival plaque" 183 : 3770-3783, 2001

24 Marsh PD, "Are dental diseases examples of ecological catastrophes?" 149 : 279-294, 2003

25 Zasloff M, "Antimicrobial peptides of multicellular organisms" 415 : 389-395, 2002

26 Beckloff N, "Activity of an antimicrobial peptide minetic against planktonic and biofilm culture of oral pathogens" 51 : 4125-4132, 2007