Inhibitory effects of Centella asiatica on the productions of NO, PGE2, TNF-α, IL-1β, and IL-6 via the suppression of NF-κB pathway in LPS-induced macrophages = LPS 매개 염증 유발 모델인 RAW 264.7 세포를 이용한 병풀(Centella asiatica)의 항염 효과 규명과 기전연구

목차 (Table of Contents)

• CONTENTS = 4
• LIST OF FIGURES = 7
• 1. INTRODUCTION = 8
• 1-1. Inflammation = 8
• 1-2. Inflammatory mediators = 9
• 1-3. Nuclear transcription factor kappa-B (NF-κB) = 12
• 1-4. Matrix metalloproteinase = 14
• 1-5. Characteristic of madecassic acid and madecassoside = 15
• 2. AIM OF STUDY = 17
• 3. MATERIAL AND METHODS = 18
• 3-1. Reagents = 18
• 3-2. Plant material and the isolation of madecassic acid and madecassoside = 19
• 3-3. Cell culture and sample treatment = 20
• 3-4. MTT assay for cell viability = 21
• 3-5. Nitrite accumulation = 21
• 3-6. Western blot analysis = 22
• 3-7. RNA preparation and RT-PCR = 23
• 3-8. Determination of PGE₂, TNF-α, IL-1β, and IL-6 production = 24
• 3-9. Nuclear extraction and electrophoretic mobility shift assay (EMSA) = 24
• 3-10. Transient transfection and luciferase assay (Reporter gene assay) = 25
• 3-11. Gelatin zymography = 26
• 3-12. Statistical analysis = 26
• 4. RESULTS = 28
• 4-1. Inhibitory effects of madecassic acid or madecassoside on LPS-induced NO and PGE₂ production = 28
• 4-2. Inhibitory effects of madecassic acid or madecassoside on LPS-induced TNF-α, IL-1β, and IL-6 release = 51
• 4-3. Inhibitory effects of madecassic acid on LPS-induced iNOS and COX-2 expressions and TNF-α, IL-1β, and IL-6 mRNA expression = 34
• 4-4. Inhibitory effects of madecassic acid on LPS-induced NF-κB-DNA binding activity, NF-κB transcriptional activity, LPS-induced nuclear translocation of NF-κB, and the phosphorylation of IκB-α = 37
• 4-5. Inhibitory effect of madecassic acid on LPS-induced MMP-9 activity = 43
• 5. DISCUSSION = 45
• 6. REFERENCES = 48
• 7. ABSTRACT = 56