알데히드화 덱스트란과 폴리라이신으로 구성된 의료용 접착제의 창상 봉합 및 지혈 효능의 평가 = Evaluation of Wound Closure and Antihemorrhagic Efficiency of Medical Adhesive Composed of Aldehyded Dextran and Poly(L-lysine)

다국어 초록 (Multilingual Abstract)

This study concentrates upon evaluating the wound closure and antihemorrhagic efficiency of a new biodegradable medical adhesive, Lydex, composed of aldehyded dextran and ε-poly(L-lysine). Each component of Lydex was mixed together and then immediately gelatinized to form an adhesive. Gel formation time of Lydex was approximately 20~30 sec at 25℃. The mechanism of Lydex gelation is based on Schiff base formation between aldehyded dextran and ε-poly(L-lysine). Lydex showed more excellent bonding strength and compressive stress than fibrin glue. The animal study in rat skin wounds (about 20 mm long) revealed that the wound closure efficiency of Lydex was superior to that of taping as (–) control after 10 d, but relatively inferior to that of Dermabond® as (+) control. The hemostatic effects of Lydex were evaluated on the rabbit liver with a surgical incision (critical-sized injury, about 20 mm long and about 5 mm deep). The hemorrhage quantity of Lydex-applied liver was significantly smaller than those of non-applied and fibrin glue-applied livers, showing that hemostasis was almost completed within 30 sec. Furthermore, when applied to surgically incised rabbit liver, Lydex bonded securely to the rabbit liver surface and tissue reaction of liver bonded to Lydex was mild without inflammation and abnormalities after 4 wk. The gel was decomposed with the progress in the time, and the complete degradation was done at 6 wk. These results suggest that a bioocompatible adhesive, Lydex could be effectively used as a medical adhesive with bleeding-stoppage function.

참고문헌 (Reference)

1 정혜순, "의료용 접착제 제조기술" 한국과학 기술정보연구원 (KISTI) 2004

2 이종호, "의료용 접착제 및 유착방지막의 최신 연구개발 동향" 한국생체재료학회 14 (14): 66-77, 2010

3 한동근, "의료용 생체접착제의 최근 연구동향" 한국과학기술 연구원 (KIST) 2005

4 김경해, "Wound Dressings for Wound Healing and Drug Delivery" 한국조직공학과 재생의학회 8 (8): 1-7, 2011

5 Y. Morita, "Visco-elastic properties of cartilage tissue regenerated with fibroin sponge" 12 : 291-298, 2002

6 G. Ciapetti, "Toxicity of cyanoacrylates in vitro using extract dilution assay on cell cultures" 15 : 92-96, 1994

7 Y. M. Hong, "The use of hemostatic agents and sealants in urology" 176 : 2367-2374, 2006

8 S. Lim, "The adhesive properties of coacervated recombinant hybrid mussel adhesive proteins" Elsevier BV 31 (31): 3715-3722, 2010

9 M. Takaoka, "Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive" 29 : 2923-2931, 2008

10 M. Morishima, "Sustained release of vancomycin from a new biodegradable glue to prevent methicillin-resistant Staphylococcus aureus graft infection" 11 : 52-55, 2010

11 P. A. Leggat, "Surgical applications of cyanoacrylate adhesives: a review of toxicity" 77 : 209-213, 2007

12 N. Nakajima, "Selfdegradable bioadhesive" 342 : 713-716, 2007

13 M. Takaoka, "Novel sutureless keratoplasty with a chemically defined bioadhesive" 50 : 2679-2685, 2009

14 R. Sethumadhavan, "Investigations on inducible nitric oxide synthase (iNOS) gene expression and SEM analysis during the interaction of a novel tissue adhesive with J774A.1 mouse macrophage cells" 17 : 159-168, 2007

15 A. Doraiswamy, "Inkjet printing of bioadhesives" 89 : 28-35, 2009

16 S. Kakinoki, "Injectable in situ forming drug delivery system for cancer chemotherapy using a novel tissue adhesive: characterization and in vitro evaluation" 66 : 383-390, 2007

17 M. Iwasashi, "In vivo evaluation of bonding ability and biocompatibility of a novel biodegradable glue consisting of tartaric acid derivative and human serum albumin" 90 : 543-548, 2009

18 M. Kaplan, "In vitro toxicity test of ethyl 2- cyanoacrylate, a tissue adhesive used in cardiovascular surgery, by fibroblast cell culture method" 8 : E169-E172, 2005

19 W. D. Spotnitz, "Hemostats, sealants, and adhesives: components of the surgical toolbox" 48 : 1502-1516, 2008

20 M. Araki, "Development of new biodegradable hydrogel glue for preventing alveolar air leakage" 134 : 1241-1248, 2007

21 H. J. Cha, "Development of bioadhesives from marine mussels" Wiley 3 (3): 631-638, 2008

22 K. -B. Bae, "Cyanoacrylate for colonic anastomosis; is it safe" 25 : 601-606, 2010

23 한동욱, "Biochemical and Histological Evaluations of Articular Cartilages Preserved in Cold Storage Solution Containing Green Tea Catechin, EGCG" 한국조직공학과 재생의학회 6 (6): 380-387, 2009

24 A. L. Tajirian, "A review of sutures and other skin closure materials" 12 : 296-302, 2010