HepG2 인간 세포주, Lumbricus rubellus 및 Saccharomyces cerevisiae를 이용한 내분비교란물질의 독성평가 = Toxicity Evaluation of Endocrine Disrupting Chemicals Using Human HepG2 Cell Line, Lumbricus rubellus and Saccharomyces cerevisiae

국문 초록 (Abstract)

다양한 독성물질 및 이들의 대사산물의 효율적인 생물독성 평가시스템이 지속적으로 개발되고 있다. 본 연구에서는 대분비계 교란물질인 endosulfan, bisphenol A, vinclozolin 및 3,5-dichloroaniline을 대상으로 인간 간암세포주, 지렁이, 효모를 이용한 세포독성 및 성장억제 효과를 평가하였다. 인간 간암세포주 독성평가에서는 endosulfan, 3,5-dichloroaniline, bisphenol A의 순으로 독성이 나타났으며, 지렁이 독성평가에서는 endosulfan, bisphenol A, 3,5-dichloroaniline의 순으로 독성이 나타났다. 효모를 이용한 독성평가에서는 3,5-dichloroaniline, endosulfan, bisphenol A의 순으로 독성이 나타나 다른 시스템과는 부분적인 차이가 나타났으며, vinclozolin의 경우 3가지 독성평가 시스템에서 모두 독성이 나타나지 않았다. 이러한 결과는, 동일한 물질을 서로 다른 생물 독성평가 시스템을 사용하여 평가하는 경우, 부분적인 오류가 나타날 수 있음을 암시하고 있으며, 독성 유무 판단은 가능하더라도, 독성 정량평가 및 독성 정도를 비교하는 것은 어렵다는 것을 제시하고 있다. 또한 본 결과는, 다양한 물질 및 이들의 대사산물의 일차적 독성평가에는 지렁이 및 효모시스템이 빠르고 경제적임을 암시하고 있으며, 독성이 인정될 경우 인간세포주 및 동물시험에 의한 검증이 효율적이라고 판단된다.

다국어 초록 (Multilingual Abstract)

Toxicity evaluation systems for various chemicals and their metabolites have been developed during last decades. In this study, the acute toxicity of endocrine disrupting chemicals, such as endosulfan, bisphenol A, vinclozolin, and 3,5-dichloroaniline, was evaluated using HepG2 cell line, Lumbricus rubellus and Saccharomyces cerevisiae, respectively. The extents of toxicity of the chemicals in different bioassay systems varied substantially, such as endosulfan>3,5-dichloroaniline> bisphenol A in HepG2 cell line system, endosulfan>bisphenol A>3,5-dichloro aniline in L. rubellus system, and 3,5-dichloroaniline>endosulfan>bisphenol A in S. cerevisiae system. Meanwhile, no cytotoxicity was observed by treatment of vinclozolin in the evaluation systems. Our results suggest that earthworm and yeast are useful to evaluate acute toxicity of endocrine disrupting chemicals, and direct comparison of toxicity data from different bioassay systems is unattainable. Based on our results, we propose that the bioassay system with earthworm or yeast, a rapid, simple and economic system, could be applied as pre-test for the toxicity evaluation using human cell line or animals.

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