PCR-RFLP에 의한 제주 재래마 미토콘드리아 cytochrome b 유전자의 유전적 다형현상 = Genetic Polymorphism of Mitochondrial Cytochrome b Gene in Jeju Native Horses Inferred from PCR-RFLP

다국어 초록 (Multilingual Abstract)

We analyzed the mitochondria1 DNA (mtDNA) cytochrome b (CytB) gene in Jeju native horses using polymerase chain reaction (PCR) mediated restriction fragment length polymorphism (RFLP). To acquire the complete sequences for the mtDNA CytB, we designed two primers based on those of prevously reported mitochondrion genome of E. caballus. The amplified PCR products were digested with 7 restriction enzymes (BamH I , Hae Ⅲ, HindⅡ, Msp I , Rsa I , Pst I and Taq I. We found two polymorphic patterns digested by Msp I and Rsa I , respectively, and the frequencies showed different between two populations of Jeju native horses. The morphs of the CytB in Jeju native horses divided into three types. This result showed similar to the previous molecular reports, but the frequencies of variations and the polymorphic positions were different. These results showed that PCR-RFLP of the mtDNA CytB is useful for detecting polymorphism and verifying the lineage of Jeju native horses.