추부깻잎의 채취시기별 GABA함량 및 항산화 활성에 관한 연구 = GABA Content and Antioxidant Activity in Chubu Perilla Leaves by Harvest Periods

다국어 초록 (Multilingual Abstract)

Perilla leaf (Perilla frutesceus var. japonica HARA) from Chubu region were extracted with water and 70% ethanol and investigated on its GABA(γ-amino butyric acid) and antioxidant activity.
GABA content of Perilla leaf were 23.37±3.22∼199.39±16.28 mg/100g. Perilla leaf in winter was higher than that of summer. GABA content of Perilla leaf in October and November were the highest 199.39 mg/100g, 130.70 mg/100g.
Total polyphenol content of Perilla leaf was 23.37 mg/mL∼80.04 mg/mL in water extracts, which were the highest 80.04 mg/mL collected Perilla leaf in August. and was 44.48 mg/mL∼201.15 mg/mL in ethanol extracts, which was the highest collection in July by 201.15 mg/mL. Polyphenol content was higher March, July and August harvested Perilla leaf. Water extract was higher than the ethanol extracts.
Superoxide dismutase like activity were 0.91∼18.71% for water extracts and were 6.38∼23.17% for ethanol extraction. perilla leaf harvested in August, respectively were as high as 18.71% and 23.78%. Collected in August to 23.78% in 70% ethanol extract was the highest. Superoxide dismutase like activity was higher in 70% ehtanol extract than water extract .
Electron donating activity of perilla leaf, the water extract was 46.46∼59.63 %, perilla leaf collected in June was the most 59.63%. the 70% ethanol extract was 59.32∼78.78 %, perilla leaf collected in June and August were the most 77.68% and 77.78%. Electron donating activity was higher in 70% ehtanol extract than water extract.
Flavonoid content in Perilla leaf was 13.04∼73.15 mg/mL in water extracts, which was the highest 73.15 mg/mL collected Perilla leaf in August, and was 22.77∼165.66 mg/mL. the highest 165.66 mg/mL collected Perilla leaf in August. Winter had a higher than Summer collected Perilla leaf.
Nirite scavenging ability at pH 4.2 were 5.00∼39.80% for water extract, perilla leaf collected in February showed the highest scavenging ability and the were 5.00∼39.80% for water extract, perilla leaf collected in February showed the highest scavenging ability. Nirite scavenging ability was higher in 70% ehtanol extract than water extract, was higher in winter than summer collected Perilla leaf.

목차 (Table of Contents)

• 목 차
• Ⅰ. 서론 1
• Ⅱ. 재료 및 방법 6
• 2.1 시료의 제조 6
• 2.2 GABA 함량 분석 6
• 2.3 항산화 활성 측정 8
• 2.3.1 총 폴리페놀 함량 8
• 2.3.2 Superoxide dismutase(SOD)활성 8
• 2.3.3 전자 공여능(Electron donating activity, EDA) 9
• 2.3.4 Flavonoid 함량 9
• 2.3.5 아질산염 소거능 9
• Ⅲ. 결과 및 고찰 11
• 3.1 GABA함량 11
• 3.2 항산화 활성 측정 13
• 3.2.1 총 폴리페놀 함량 13
• 3.2.2 Superoxide dismutase(SOD)활성 측정 14
• 3.2.3 전자 공여능(Electron donating activity, EDA) 측정 16
• 3.2.4 Flavonoid 함량 17
• 3.2.5 아질산염 소거능 18
• Ⅳ. 적 요 21
• 참고문헌 23
• Abstract 28