Screening and Cloning of Phytochrome Genes from Soybean (Glycine max L.) Seedlings

다국어 초록 (Multilingual Abstract)

To understand the molecular function of phytochrome in photomorphogenesis, isolation of phytochrome genes from soybean (Glycine max) was attempted by plaque hybridization with a cDNA clone of Cucurbita phytochrome. Soybean genomic DNA library was constructed into λ EMBL 3 vector with soybean total DNA isolated from young soybean seedlings. Two phytochrome genes were isolated from the genomic library and Southern blot analysis was carried out. The result of partial sequencing and restriction endonuclease analysis showed that two genes are different but quite homologous each other. These clones, A4 and B3, encode the entire amino acids sequence for soybean phytochrome including 5' and 3' flanking regions. Genomic Southern blot analysis suggest that there are at least three different phytochrome genes in soybean. Northern blot analysis revealed that polyadenylated phytochrome transcript (- 4.5 kb) is present in the leaves of soybean grown in dark rather than in light.