The Optical Measurement and Quantitative Analysis of Algesia in Spodoptera litura Larva

다국어 초록 (Multilingual Abstract)

Muscle vibration measurement has long been an unique scientific study, in general, and the directreaction of animals to feel pain (algesia), either from vascular or muscle contraction, is a complexperceptual experience. Thus this paper proposes a way to measure animal algesia quantitatively, bymeasuring the changes in muscle vibration due to a pinprick on the surface of the skin of a Spodopteralitura larva. Using the laser optical triangulation measurement principle, along with a CMOS image sensor,linear laser, software analysis, and other tools, we quantify the subtle object point displacement, with aprecision of up to 10 μm, for our chosen Spodoptera litura larva animal model, in which it is not easyto identify the tiny changes in muscle contraction dynamics with the naked eye. We inject differentconcentrations of formalin reagent (empty needle, 12% formalin, and 37% formalin) to obtain a varietyof different muscle vibration frequencies as the experimental results. Because of the high concentrationsof reagent applied, we see a high frequency shift of muscle vibration, which can be presented as painindices, so that the algesia can be quantified

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