소함흉탕 에탄올 추출물 및 황련 알칼로이드의 헬리코박터 파이로리에 대한 항균활성 = In Vitro Anti-Helicobacter pylori Activity of Ethanol Extract of Sohamhyoongtang and Coptidis Rhizoma Total Alkaloids

다국어 초록 (Multilingual Abstract)

The aim of this study was to evaluate the anti-helicobacter activity of the ethanol extract of Sohamhyoongtang(Coptidis Rhizoma, Pinelliae Tuber and Trichosanthis Semen) and Coptidis Rhizoma total alkaloids, which is one of the componentsof Sohamhyoongtang. Crude ethanol extract of Sohamhyoongtang (ESHHT) and Coptidis Rhizoma total alkaloids(CRTA) were used for this experiment. Five different types of H. pylori (including H. pylori 26695) were used as test strain.
To determine anti-helicobacter activity, minimum inhibitory concentration (MIC) was determined by agar dilution method. Theeffect of ESHHT and CRTA on the gene expression of H. pylori was investigated by quantitative realtime-PCR (qRT-PCR).
MICs of ESHHT against five H. pylori strains were 250~500 µg/ml and MICs of CRTA against five H. pylori strains were50~200 µg/ml. Four representative virulence genes of H. pylori, cagA, ureA, ureB and ureI were tested as target genes for qRTPCR.
According to the qRT-PCR results, both ESHHT and CRTA markedly repressed the expression of cagA gene of H. pylori26695 (6.91 and 20 folds respecively). These results showed that the ESHHT and CRTA demonstrated antihelicobacter properties,suggesting their potential use in gastritis or duodenal ulcer.

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