우엉 뿌리 추출물의 항산화 및 Tyrosinase 저해 활성과 Phenolic Compound 분석 = Antioxidative Activity and Tyrosinase Inhibitory Activity of the Extract and Fractions from Arctium lappa Roots and Analysis of Phenolic Compounds

다국어 초록 (Multilingual Abstract)

In this study, we investigated on antioxidative activities and tyrosinase inhibitory activities of methanol extract andits fractions from roots of Arctium lappa. The total phenolic compound and flavonoid content of the ethylacetate fraction wasfound to be 818.29 mg/g and 360.59 mg/g as the highest content. In the measurement of DPPH radical scavenging ability andtyrosinase inhibitory activity, the ethylacetate fraction was higher than the other fractions and the extract. In addition, comparativeanalysis of phenolic compounds by liquid chromatography-mass spectrometry (MS)/MS system under the multiplereactionmonitoring (MRM) with negative-ion electrospray ionization mode. The main phenolic compounds in the extract andfractions of roots from Arctium lappa were cynarin and chlorogenic acid. The main phenolic compound of the ethylacetate fractionwas cynarin. n-Butanol fraction had a significantly higher chlorogenic acid content than other samples. In conclusion,DPPH radical scavenging ability and tyrosinase inhibitory activity of the cynarin-riched ethylacetate fraction showed the highest activity.

참고문헌 (Reference)

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