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국문 초록 (Abstract)

최근 3D 바이오프린팅은 기존의 제작 방법으로는 표현하기 어려웠던 조직 구조의 정밀하고 세밀한 기계적 움직임을 통해 실제 장기 모델을 제작할 수 있는 기술로 기대되고 있다. 따라서 인...

최근 3D 바이오프린팅은 기존의 제작 방법으로는 표현하기 어려웠던 조직 구조의 정밀하고 세밀한 기계적 움직임을 통해 실제 장기 모델을 제작할 수 있는 기술로 기대되고 있다. 따라서 인쇄성과 생체적합성이 향상된 레이저 기반 바이오프린팅 플랫폼에 대한 연구가 필요했다. 생체 적합성을 최우선으로 높은 인쇄성을 나타낼 수 있는 최적화 연구를 통해 이상적인 GelMA 기반 바이오 잉크와 최적화된 인쇄 플랫폼을 개발했습니다. 최적화 전략을 통해 구현된 바이오프린팅 플랫폼을 이용하여 프린팅된 GelMA 지지체 내의 세포 환경을 확인하였고, 본 연구에서 구축한 플랫폼의 효용성을 대량 배양을 통해 검증하였다.

다국어 초록 (Multilingual Abstract)

Recently, 3D bioprinting is expected as a technology capable of producing an actual organ model through precise and detailed mechanical movement of a tissue structure, which was difficult to express with conventional fabrication methods. Therefore, it was necessary to study a laser-based bioprinting platform with improved printability and biocompatibility. We developed an ideal GelMA-based bio-ink and an optimized printing platform through research on optimization that can show high printability, with biocompatibility as the top priority. Using the bioprinting platform implemented through the optimization strategy, the cellular environment in the printed GelMA support was confirmed and the utility of the platform established in this study was verified through mass culture.

목차 (Table of Contents)

제1장 서론 1
제1절 Functional biomaterial: hydrogel 1
1. Introduction of hydrogel 1
2. History of hydrogel 1
3. Natural polymer hydrogel 3

제1장 서론 1
제1절 Functional biomaterial: hydrogel 1
1. Introduction of hydrogel 1
2. History of hydrogel 1
3. Natural polymer hydrogel 3
제2절 Application of functional natural polymer hydrogel: bioprinting 8
1. Introduction of bioprinting 8
2. 3D bioprinting process 9
3. Types of 3D bioprinting 11
4. Application of 3D bioprinting 14
제2장 Light/temperature sensitive hydrogel platform for stereolithographic printing 16
제1절 서론 16
제2절 재료 및 방법 19
1. Materials 19
2. Preparation of HBC-MA solution and hydrogel 19
3. 1H nuclear magnetic resonance spectroscopy 20
4. Fourier transform infrared spectroscopy 21
5. Scanning electron microscopy 21
6. Reversible phase transition characterization of hybrid-crosslinked hydrogel 21
7. Rheological analysis 22
8. Mechanical testing 22
9. Swelling test 22
10. Hydrogel degradation 23
11. In vitro hydrogel crosslinking test 23
12. Live/Dead fluorescence assay 24
13. Cell counting kit-8 assay 25
14. Preparation of 4D resin formulation 25
15. SLA-3D printing setting 26
16. Preparation of 3D models 27
17. Morphological studies 27
18. Swelling properties 27
19. Mechanical properties 28
20. Calculation of bending ratio of 4D structures 28
제3절 결과 및 고찰 29
1. 1H Nuclear magnetic resonance spectroscopy 29
2. Fourier transform infrared spectroscopy 30
3. Morphological analysis 31
4. Reversible characteristics of HBC-MA hydrogel 33
5. Rheological analysis 34
6. Mechanical analysis 35
7. Swelling test 37
8. Degradation test 38
9. Live/Dead fluorescence assay 40
10. Cell counting kit-8 assay 41
11. SLA-3D printing process and resin component 42
12. 3D modeling for 4D structure 44
13. Morphological analysis of printed hydrogel structure 45
14. Swelling analysis of printed hydrogel structure 45
15. Mechanical analysis of printed hydrogel structure 48
16. Bending ratio of 4D structure evaluation 49
제4절 결론 52
제3장 Cell-laden gelatin methacryloyl bioink and laser-type printing platform for the biofabrication of centimeter-scale hydrogel scaffolds 53
제1절 서론 53
제2절 재료 및 방법 55
1. Materials 55
2. Cell culture 55
3. Gelatin methacryloyl synthesis 56
4. Preparation and fabrication of bioink 56
5. Modeling 57
6. 1H nuclear magnetic resonance spectroscopy 57
7. UV-vis spectrometer measurement 57
8. Photorheological analysis 58
9. Printability test 58
10. Live/Dead assay according to light exposure time 60
11. Scanning electron microscopy 60
12. Mechanical testing 60
13. Cell adhesion 61
14. Cell proliferation assay 61
15. Statistical analysis 61
제3절 결과 및 고찰 62
1. 1H nuclear magnetic resonance spectroscopy 62
2. GelMA bioink preparation for 3D bioprinting 64
3. DLP 3D bioprinting optimization: photorheological analysis 65
4. Scanning electron microscopy 69
5. Cell adhesion in DLP printed GelMA scaffolds 71
6. Cell viability in DLP printed GelMA scaffolds 73
7. Cell proliferation analysis 75
8. Application study 1: Ear-shaped DLP scaffold printing and cell culture 75
9. Application study 2: Centimeter-scale scaffold for steak-type cultured meat 78
제4절 결론 81
제4장 Conclusion 83
참고문헌 86
Abstract (in Korean) 104

참고문헌 (Reference)

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